Undergraduate Research Assistant
Conducted cloning of SRSF1 deletion mutants to test hypothesis on SNAIL's role in mediating alternative splicing via SRSF1.
Examined specific domains in SRSF1 to determine essential regions for binding with SNAIL.
Extracted and amplified distinct mutant SRSF1 plasmid DNA from cDNA.
Employed restriction enzymes to integrate purified mutant plasmid DNA into cloning vectors via ligation.
Achieved transformation of competent E. coli cells using cloned vectors carrying SRSF1 plasmid mutants.
Digested tranformed vectors to screen for mutant clones.
Validated clones via Sanger sequencing to confirm successful cloning.