Erick Galdamez Calles
Los Angeles,CA
Summary
Currently working as a Research Associate and laboratory technician with a passion for research and enthusiasm about driving drug discovery and advancing knowledge in Biomedical and Biochemical processes. Graduate at California State University, Dominguez Hills, offering experience in research support tasks such as, biochemical assays, completing analyses, quantifying data, aseptic technique, GMP/GLP, analytical development, protocol development and optimization and using biochemical processes to complete specific research experiments.
Overview
4
4
years of professional experience
Work History
Research Associate
Ophidion Bio
Pasadena, CA
01.2022 - Current
- Experienced in a range of responsibilities within a laboratory setting, specializing in cell culture, cell line maintenance(HEK 293T, SH-SY5Y, GBM, U87), passaging, banking, and cell differentiation.
- Analyze the impact of Ophidion compounds on gene and protein expression, utilizing Microsoft Excel, Google Sheets, ImageJ, NovoExpress, quantstudio and Agilent chemstation for data analysis, interpretation, and visualization stemming from qPCR, fluorescence assays, Flow Cytometry, gel electrophoresis and HPLC.
- Demonstrating strong technical proficiency across multiple disciplines, including cell biology (cell culture), chemistry (HPLC), and molecular biology (RNA extraction, cDNA generation, qPCR, Western blot, EMSA, ELISA), Flow Cytometry.
- Responsible for the maintenance, operation, and troubleshooting of essential analytical equipment and instruments such as qPCR machines, fluorescence scanners, and pipettes
- Ensure the continuous availability, inventory, and quality of reagents, supplies, and instruments in the laboratory.
- Design, development, optimize, and research of SOPs.
- Actively engage with vendors, establish connections within the industry through conference participation, and online connections, and nurture networking relationships with various companies.
- Trained and mentored interns from community colleges (Pasadena city college) and universities (Cal state Northridge) in basic cell culture techniques, including aseptic technique, media preparation, and handling cell lines (293T, SH-SY5Y)
- Trained interns to be able to perform in vivo experiments such as cell treatments, RNA extractions, cDNA synthesis, and qPCR amplification
- Performed microscopy, capturing cell images and using fluorescence microscopy to observe cell transfections with eGFP mRNA and fluorescent dyes.
Laboratory Technician
Viridian Biometals formally Impossible Metals
Pasadena, CA
01.2023 - Current
- Develop, optimize, and research protocols for implementation in HPLC analysis.
- Analyze Viridian bio samples for short-chain fatty acid (SCFA) quantification using HPLC.
- Maintain HPLC equipment.
- Actively engage with vendors, establish connections within the industry through conference participation, and nurture networking relationships with various companies.
- Prepare for weekly experiments which include the making of various Viridian specific media, acids.
- Keeping and maintaining certain glassware sterile through autoclaving to be used in experiments, preparing samples for ICP-OES testing.
- Disposal of biohazardous waste through acid neutralization and autoclaving.
Manufacturing Associate
Pendulum Therapeutics
San Francisco, CA
06.2021 - 11.2021
- Managed the setup, operation, and breakdown of large-scale fermenters on a weekly basis, with a focus on in-process fermentation, predominantly using single-use bioreactors
- Maintained vigilant monitoring throughout the fermentation process
- Proficiently operated and ensured the accurate completion of batch records, adhering to cGMP and SOP standards
- Executed seed train processes within an anaerobic hood, commencing with plate streaking and transferring to 8ml and 500ml tubes
- Accurately weighed and prepared chemicals, medium/buffer solutions, and facilitated the transfer of media from nonsterile single-use bags to sterile single-use bags via filter sterilization
- Conducted aseptic/sterile inoculations of single-use bioreactors, closely monitoring and adjusting parameters as necessary
- Oversaw the bacterial harvesting process through centrifugation and operated lyophilizer to sublimate the product
- Employed the CIP cart to maintain the cleanliness of lyophilizer and centrifuge after each use
- Ensured departmental adherence to the 5S rule, maintaining a clean and organized workspace
Education
BS - Biochemistry Minor Mathematics
California State University, Dominguez Hills
Carson, CA05-2021
Skills
- HPLC
- Aseptic environment
- Imortalized Human/mammalian cell line culturing
- Q/rt PCR
- Flow Cytometry
- Microsoft Office
- SOP and protocol development
References
- Brigitte Angenieux, Executive Director of Preclinical Development at Ophidion Bio, (310) 954-6391
- Khashayar Goshtasb, Director of Operations at Viridian Biometals, (408) 859-7737
- Wendie Johnston, Lab Director at Pasadena Bio Collaborative Incubator, (626) 644-1412
Publications
CARF (CDKN2AIP) Regulates Hepatic Lipid Metabolism and Protects Against Development of Non-Alcoholic Fatty Liver Diseases, Kamrul M Hasan, PhD, Meher Parveen, Ph.D, Alondra Pena, BS, Erick Galdamez Calles, BS, Marvy Gergis, MS, Jorge Espinoza-Derout, PhD, Amiya Sinha-Hikim, MS, PhD, Theodore C Friedman, MS, MD, PhD, Journal of the Endocrine Society, 5, Supplement_1, 04/2021, A284, https://doi.org/10.1210/jendso/bvab048.577
Languages
- Fluent in English and Spanish
- Beginner level Portuguese
Languages
Spanish
Native/ Bilingual
Portuguese
Limited
References
References available upon request.
Timeline
Laboratory Technician
Viridian Biometals formally Impossible Metals
01.2023 - Current
Research Associate
Ophidion Bio
01.2022 - Current
Manufacturing Associate
Pendulum Therapeutics
06.2021 - 11.2021
BS - Biochemistry Minor Mathematics
California State University, Dominguez Hills
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