Summary
Overview
Work History
Education
Skills
Awards
Accomplishments
Timeline
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Ethan Constantine

McKinney,Tx

Summary

6 years of research experience with laboratory and analytical skills such as PCR (16s & qPCR), ELISA, DNA extraction, R coding, microbiome analyst, and more. Highly proficient with Microsoft software (word, excel, teams, PowerPoint). Experience with writing protocols and published two manuscripts. Excels with meeting deadlines and working on multiple projects at the same time.

Overview

7
7
years of professional experience

Work History

Lab Instructor

12.2021 - 05.2024
  • Taught lab courses for microbiology, microbial ecology, and microbial physiology for 3 years.
  • Mentored undergraduate students to conduct their own independent research projects relating to microbiology and the healthcare industry.
  • Taught students how to use specific lab techniques in the lab, such as the aseptic technique, differences between growth media, R coding, and reading/interpreting scientific papers. Also, taught students the importance of sterilization and how to manage different microorganisms.ms
  • Many of these students were applying to nursing and professional schools.

Research Assistant

08.2021 - 04.2024
  • Conducted research as an RA to observe the effect of vitamin D on gut integrity and gut microbiome composition using C57BL/6J mice as a model (IACUC #8007)
  • The study utilized protocols for 16S PCR, qPCR, ELISA, and DNA extraction
  • Met specific deadlines for PCR sequencing to stay on schedule when reporting data to the Nutrition department
  • Research required creating buffers and reagents for PCR analysis specifically for electrophoresis gels
  • I helped to organize and coordinate projects between the nutrition program and microbiology department at Texas state, while also fully participating in lab work of both labs
  • Was in charge and responsible for maintaining and keeping records of all lab inventory and kept lab up to state safety standards when evaluated by the department.

Research Assistant

08.2017 - 12.2020
  • Conducted research as an RA studying the effects of mir-214 on osteoblasts using medaka fish as a mode
  • Required the use of microinjections, electron microscopy, image J software, and efficient use of Microsoft applications
  • I Also have some experience using mass spectrometry.

Education

Master of Science - Biology

Texas State University
San Marcos, TX
05.2024

Bachelor of Science - Biomedical Sciences

Texas A&M University - Corpus Christi
Corpus Christi, TX
12.2020

Skills

  • Microsoft applications (excel, word, PowerPoint, & teams)
  • Statistical software and data analysis (R studios and microbiome analyst)
  • Leadership skills
  • Made buffers and reagents in lab (ex: electrophoresis gels)
  • Kept organized and detailed notebooks and records of laboratory protocol to report work to higher staff
  • Multitasking (worked in multiple labs to effectively complete projects that were coordinated between the biology and nutrition department at Texas State)
  • Maintain clean labs and workspaces
  • Follow SOP exactly while also being able to adjust SOP depending on the situation to fix / troubleshoot lab procedure (ex 16s PCR sequencing and DNA extractions)
  • Read and summarize scientific literature and utilize knowledge from research papers for my own work
  • Worked with animals (mice & rats) as a model for study (IACUC protocol)
  • Presented at conferences such as American Society of Biochemistry and Molecular Biology (ASBMB)
  • Mass spectrometry
  • Electron microscopes
  • Instrument Calibration
  • Laboratory Management
  • Biological Specimen Handling
  • Sample Preparation
  • Multitasking
  • Analytical Skills
  • Academic Research
  • Grant Writing
  • Academic Publication
  • Research oversight
  • Time Management
  • Organizational Skills
  • Professionalism
  • Research Paper Writing
  • Data Analysis
  • Document Management
  • Recordkeeping
  • Statistical Analysis
  • Data Collection
  • Lab Safety
  • Laboratory Testing
  • Tissue Processing
  • Specimen Preparation
  • Research and analysis
  • Specimen handling
  • Teaching students
  • Quantitative and qualitative analysis
  • Managing laboratory supplies
  • Equipment Maintenance
  • Public presentations
  • Analyzing and synthesizing data
  • Problem-Solving
  • Flexible Schedule
  • Publication Preparation

Awards

  • Nominated for student employee of the year award in April 2024 for my work as a lab instructor.
  • Awarded the student government scholarship award in January 2023.
  • Awarded the Dorothy Cocker Research Fellowship in February 2023 for funding of my research.

Accomplishments

  • Published two manuscripts “Combination of vitamin D3 and fructooligosaccharides upregulates colonic vitamin D receptor in C57BL/6J mice and affects anxiety-related behavior in a sex-specific manner” & “Supplementation of vitamin D3 and Fructooligosacharides downregulates intestinal defensins and reduces the species abundance of romboutsia ilealis in C57BL/6J mice” for the Journal of Nutrition. (nutrients-16-02236.pdf, 2024_Nutr Res_Combination of VD and FOS upregulates colonic VD receptor in mice.pdf)

Timeline

Lab Instructor

12.2021 - 05.2024

Research Assistant

08.2021 - 04.2024

Research Assistant

08.2017 - 12.2020

Master of Science - Biology

Texas State University

Bachelor of Science - Biomedical Sciences

Texas A&M University - Corpus Christi

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Ethan Constantine