Ethan Rothschild
Mount Laurel,NJ
Summary
Laboratory scientist offering background in collecting, processing and analyzing various specimens. Familiar with basic medical terminology, laboratory protocols and testing procedures. Expertise in qPCR, RT-qPCR, nucleic acid extraction, and analysis. Recognized for performing laboratory tests and procedures with attention to detail, optimum quality and expedience.
Overview
8
8
years of professional experience
Work History
Senior Laboratory Technician
Wuxi AppTec
08.2022 - 04.2023
- Performed molecular biology technique to fulfill external and internal contracts for a growing CDMO
- Worked with viral vectors provided by clients in a BSL-2+ lab environment with a highly comprehensive policy of GLP, cGMP, GDP, and GTP
- Performed different DNA and RNA isolation methods for DNA qPCR and RNA RT-qPCR assays on client- provided test article to assay for viral nucleotides
- Carried out data analyses using Applied Biosystems 7500 Fast Real-Time PCR equipment and software
- Validated nucleotide extraction and qPCR procedures in a newly-operational laboratory
- Performed validation of new varieties of test article and qualifications of new and current lab reagent
- Trained in performing ABI AutoMate Express DNA extraction, QIAsymphony DNA extraction, Tecan Fluent automated liquid handling for qPCR plate preparation, and MycoSeq mycobacterium DNA detection procedure.
Laboratory Technician III
WuXi AppTec
03.2021 - 08.2022
Laboratory Technician II
WuXi AppTec
05.2019 - 03.2021
- Collected and performed analytical testing on in-process product.
- Supported laboratory staff with advanced troubleshooting, resolving complex problems and helping with special projects.
- Maintained documentation on tests performed in lab.
- Interpreted and documented results of tests into active databases and communicated results to internal customers.
Analytical Scientist (Contractor)
Spark Therapeutics, Inc
09.2018 - 05.2019
- Applied molecular biology technique towards assaying the production of gene therapy vector
- Handled HEK-293 and adeno-associated viral vector stocks in a BSL-2 lab environment that required the practice of GLP and cGMP
- Helped advance Spark Therapeutics research into the production of gene therapy vectors to cure Pompe Disease and Hemophilia A/B
- Acquired an expertise in performing qPCR of viral DNA along with accompanying data analysis to measure titers
- Used QuantStudio 7 equipment and software.
- Formulated reagents needed for each day's experiments, developed technique in preparing qPCR samples from upstream and downstream production sample sets, and maintained a lab notebook and proper filing of data in the company storage drive.
R&D Lab Associate
Akers Biosciences, Inc
09.2015 - 07.2018
- Applied biochemistry towards developing and testing medical devices for the commercial market
- Worked with human blood, serum, BSL-2 pathogens, organic chemistry, GLP, and cGMP
- Participated on a research team that studied how to test patients for heparin-induced thrombocytopenia risk, oxidative stress, ketone concentration, asthma, and chlamydia
- Performed ELISA, SDS-PAGE, spectroscopy, protein extraction and purification from biological samples, formulation of reagent for test kits, programming of electronic medical devices, feasibility testing, and validation testing
- Formulated buffer solutions, produced reagents, and experimented with metallic catalysis of peroxide compounds
Education
Master of Science - Microbiology And Immunology
Rutgers University
New Brunswick, NJ05.2014
Bachelor of Science - Biology
The College of New Jersey
Trenton, NJ05.2012
Skills
- Work Planning and Prioritization
- Sample Preparation and Analysis
- Inventory Control
- Analyze Body Fluids
- Specimen Examination
- Laboratory Support
- Documentation Management
- Sanitization Protocols
- Customer Satisfaction
- Molecular Diagnostics
- Processing Specimens
- Testing Specimens and Samples
Additional Information
Rothschild, Ethan, and Debabrata Banerjee. "Subverting subversion: a review on the breast cancer microenvironment and therapeutic opportunities." Breast cancer: basic and clinical research 9 (2015): BCBCR-S29423.
Timeline
Senior Laboratory Technician
Wuxi AppTec
08.2022 - 04.2023
Laboratory Technician III
WuXi AppTec
03.2021 - 08.2022
Laboratory Technician II
WuXi AppTec
05.2019 - 03.2021
Analytical Scientist (Contractor)
Spark Therapeutics, Inc
09.2018 - 05.2019
R&D Lab Associate
Akers Biosciences, Inc
09.2015 - 07.2018
Master of Science - Microbiology And Immunology
Rutgers University
Bachelor of Science - Biology
The College of New Jersey
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