Evelyn Subroto
Scientist/Research Associate
Summary
Determined and well-rounded individual with 2 years working as a Scientist within the biotech industry. Sharp and focused professional offering skills in Next Generation Sequencing and Clinical Sample Processing. Highly motivated with outstanding organization skill and attention to detail.
Overview
4
4
years of professional experience
2
2
Languages
Work History
Scientist 1
Roche Diagnostics
Pleasanton, United States
08.2021 - Current
- Led a DNA Inventory project where a microtome was used to cut 193 FFPE and Xenograft blocks. Each block produced ~30-40 tubes of 4x 20nm curls each. 24 tubes were bulk extracted, sheared, and placed into inventory for broad team usage. A poster was made and presented describing the workflow and purpose of this project.
- Independently conducted experiments involving library preparation of cfDNA, FFPE, or cell-line DNA samples and sequenced resulting libraries on the Illumina Novaseq platform. Results were analyzed using JMP, Illumina SAV and were compiled into slide decks that were presented to subchapter.
- Ordering reagents and consumables necessary for consistent lab workflow and inputting orders into a team inventory system.
- Documented changes during experiments, noting unexpected issues arising during research activities.
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation.
- Followed strict SOPs, protecting staff and assets while carrying out technical procedures and research protocols.
Research Lab Room Coordinator
Roche Diagnostics
Pleasanton, United States
01.2022 - Current
- Maintained lab tidiness based on strict SOPs. Includes: Tidying up the lab room i.e wiping down benches with bleach/ethanol, defrosting and reorganizing CTUs, disposal of medical waste to appropriate waste room, maintaining pipette calibration and recording presence of active/non-active pipettes found in this space, maintaining proper lab documentation in a 5-point inspection binder.
- Trained 20 new users in lab to expected SOP levels based on trainee position.
- Re-stocked laboratory consumables as needed on a bi-weekly basis i.e pipette tips, water bottles, Qubit tubes/various tubes, biohazardous waste bins, reagent reservoirs, etc.
- Ordering equipment/reagents as needed to maintain adequate stock of reagents i.e Tapestation/BioAnalyzer reagents + accessories, Qubit reagents, microfuges, vortexers, etc
- Led large scale reorganization of lab involving moving/removing equipment with assistance from RSS systems team.
- Ensured that all equipment was in working condition and reaching out to repair team when an instrument was not working properly.
Clinical Laboratory Intern
KalGen InnoLab
Jakarta, Indonesia
07.2019 - 08.2019
- Tracked collected specimens by initializing, dating, and noting times of collection and arrival to laboratory
- Prepared blood smears and other specimen cultures to determine and report levels of pathogenicity to supervisors
- Gram stained 150+ slides and determined present bacteria with supervision
- Logged patient information and recorded results into excel and printed out labels to place onto respective samples.
- Labeled specimens and delivered them across departments.
- Independently prepared cell culture media for microbiology department; primarily preparing LB agar plates in bulk weekly.
Education
Master of Science - Biomedical Sciences
08.2020 - 08.2021
Bachelor of Science - Cellular And Molecular Biology
09.2016 - 06.2020
Skills
Microsoft Office/Google Workspace
Next Generation Sequencing (Illumina Novaseq)
Assay Development: Library prep, qPCR, PCR
Microtome (FFPET/Xenograft cutting)
FFPET, Xenograft, cell-line, cfDNA/Plasma extractions
Mechanical DNA Shearing (Covaris inc)
Enzymatic DNA Shearing (KAPA Biosystems)
Safety and environmental standards
Timeline
Research Lab Room Coordinator - Roche Diagnostics
01.2022 - Current
Scientist 1 - Roche Diagnostics
08.2021 - Current
California Baptist University - Master of Science, Biomedical Sciences
08.2020 - 08.2021
Clinical Laboratory Intern - KalGen InnoLab
07.2019 - 08.2019
University of California, Santa Cruz - Bachelor of Science, Cellular And Molecular Biology
09.2016 - 06.2020