Catherine Joseph

• Department: Department of Immunology
• Trained in various Molecular techniques (PBMC isolation, FACS sorting, Western Blot, Bacterial Culture, SDS-PAGE)
• Assisted Scholars in developing a recombinant BCG vaccine to circumvent the challenges posed by Antibiotic resistant Tuberculosis

• Department: Department of Human Genetics
• Trained in Karyotyping, identification and analysis of Chromosomal abnormalities and introduction to Genetic Counselling

• Anatomy and Physiology 1

Functional and Structural Analysis of the Receptor-Binding site of Cry1Ab Toxins of Bacillus thuringiensis

• Operating and maintaining state-of-the-art laboratory equipment and instrumentation, ensuring optimal performance and accuracy (AKTA)
• Conducting experiments according to established protocols, recording and analyzing data meticulously, and preparing detailed reports of experimental findings (SDS-PAGE, Bacterial Transformation, Mutagenesis, Molecular Cloning, Western Blot, Qualitative and Quantitative Assays)
• Collaborating with fellow researchers to design and troubleshoot experiments, brainstorm innovative approaches, and interpret results effectively
• Mentored and taught undergraduates to support collaborative learning and further promote educational unit objectives

Evaluation of Heme Inhibitory therapy in combination with radiation of Lung Cancer

• Performing immunohistochemical (IHC) staining assays to assess the expression levels and subcellular localization of specific biomarkers implicated in lung cancer progression and treatment response
• Conducting histological evaluations of lung cancer specimens using hematoxylin and eosin (H&E) staining to visualize tissue architecture, cellular morphology, and pathological features, allowing for comprehensive assessment of tumor grade, stage, and histological subtypes
• Analyzing histopathological and immunohistochemical data using digital image analysis software to quantify staining intensity, distribution patterns, and morphometric parameters

• Key project: Evaluation of TLR2 gene polymorphism for its influence in Helicobacter pylori induced Gastric Cancer and Gastric ailments
• Implementing state-of-the-art genotyping technologies, such as genome-wide association studies (GWAS) to interrogate the genetic landscape of gastric cancer patients and control cohorts
• Successfully perform everyday experiments like DNA Extraction, ELISA, DNA quantification, PCR, Genotyping and Restriction Digestion