Summary
Overview
Work History
Education
Skills
Language
Timeline
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Forest Chen

San Jose,CA

Summary

Experienced laboratory scientist adept in academic and personal research endeavors. Demonstrated proficiency in multitasking, effective communication, and collaborative teamwork. Possesses a proven track record of independent initiative coupled with strong team integration skills.

Overview

5
5
years of professional experience

Work History

Research Associate

ASC Therapeutics
San Jose, CA
09.2021 - 10.2023
  • Next Generation Sequencing (NGS) using CHANGE-Seq, CIRCLE-Seq, GUIDE-Seq, rhAMP-Seq, and Nextera-based sequencing for projects using Illumina's MiniSeq.
  • Transformation of E. coli and downstream assays including Giga, Maxi, and Mini prep assays for isolation of plasmid constructs.
  • Experienced in CRISPR/CAS9 and supporting scientist with designing primers, executing genotyping.
  • Cell Culturing of Hepa G2, RF/6A, Hepa 1-6 cells including
  • Preparation of liver tissue samples then homogenized for preparation for gDNA and RNA extraction.
  • cDNA preparation of RNA from mouse/monkey tissue and cells.
  • RT-qPCR to quantify knock-in expression levels of the HBB gene using SYBR green dye.
  • BCA and Western Blot assays to quantify target SaCas9 and KI products in mice.
  • Independently designed and executed experiments at the request of project leaders when given different sequences to test in different animal cells.

Pharmacy Intern

Eagle Drug
Winters, CA
01.2020 - 03.2020
  • Maintained records of controlled substances and monitored inventory levels.
  • Organized medications promptly and accurately according to patients' orders following established procedures.
  • Assisted pharmacists and pharmacy technicians with labels, records, inventory organization, delivery preparation and administration.
  • Liaison between patients and pharmacy technicians, improving communication and efficiency.

Teaching Assistant

Davis Senior High School
Davis, CA
03.2019 - 06.2019
  • Assisted teachers and assessed students on their abilities and lab competence.
  • Hosting additional office hours outside of regular class sessions.
  • Applying previously acquired scientific knowledge demonstrates its retention and efficacy.

Education

Bachelor of Science - Biochemistry And Molecular Biology

University of California - Davis
Davis, CA
03-2021

Skills

  • Next Generation Sequencing (NGS): CHANGE-Seq, CIRCLE-Seq, GUIDE-Seq, rhAMP-Seq, WGS
  • CRISPR using SaCas9 and sgRNA variants
  • Transductions (AAV6 and AAV8 serotypes) and Transfections
  • Cell Culturing of Hepa G2, RF/6A, Hepa 1-6 cells
  • PCR, RT-qPCR (using SYBR master mix)
  • E Coli Transformation
  • In-Fusion Cloning and Molecular Cloning using Stellar Cells
  • Giga, Maxi, and Mini preps of bacteria
  • RNA and DNA bioanalysis
  • Mouse Liver Tissue processing, homogenization, sonication, and collection of DNA, RNA, and Protein
  • cDNA synthesis and RNase-treatment
  • Qubit assay and Nanodrop assay
  • Western Blot
  • Experienced using R Studio
  • Proficient in Microsoft Office (Word, Excel, PPT)

Language

English - Native/Bilingual

Mandarin - Native/Bilingual

Timeline

Research Associate

ASC Therapeutics
09.2021 - 10.2023

Pharmacy Intern

Eagle Drug
01.2020 - 03.2020

Teaching Assistant

Davis Senior High School
03.2019 - 06.2019

Bachelor of Science - Biochemistry And Molecular Biology

University of California - Davis

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Forest Chen