Ph.D. - Molecular Biosciences
University of Texas At Austin
Austin, TX07-2022
Foteini Bartzoka
Newton,MA
Summary
Versatile and detail-oriented professional with educational background in Molecular Biosciences and emphasis on Protein Biochemistry and Applied Immunology scientific concepts. Recent graduate with excellent research, technical and problem-solving skills. Detail-oriented and able to learn new concepts quickly.
Education
M.Sc. - Systems Biology
Agricultural University of Athens, Greece
02-2016
B.Sc. - Agricultural Biotechnology
Agricultural University of Athens, Greece
10-2014
Skills
- Construct design for recombinant protein expression in prokaryotic and eukaryotic systems
- Molecular biology techniques ie, restriction digestion cloning, Gibson assembly, GoldenGate, Polymerase Chain Reaction (PCR)
- Sanger Sequencing, Next Generation Sequencing and immune repertoire sequencing (ie MiSeq 2x300, PacBio) and sequencing analysis
- Protein and antibody purification; purification of tagged (6-His, FLAG-tag, Strep-tag, HGH-tag etc, protein A and G) and tag-less constructs (ion exchange chromatography, preparative and analytical size-exclusion chromatography)
- Operation of automated purification using AKTA FPLC systems
- Mammalian cell culture, maintenance and transfection (ie CHO, HEK293, A431, A549, MCF7 etc)
- Cell based signaling assays
- Antibody-antigen interaction characterization (ie Surface Plasmon Resonance (SPR), Bio-layer interferometry (BLI))
- Protein identification with SDS-PAGE, Western Blot and ELISA
- Sample preparation for Mass Spectrometry analysis
- Experience in interpretation of Mass Spectrometry data using Proteome Discoverer
- Generation & characterization of natively paired antibody heavy and light chain transcripts and B-cell receptor repertoire high-throughput sequencing from peripheral blood mononuclear cells (PBMCs)
- Generation of water-in-oil emulsions for the encapsulation of single-cells
- Immunofluorescence and flow cytometry assays
- Protein sample preparation for X-ray crystallography and crystal screening
- Cryo-electron microscopy sample preparation, data collection, cryo-electron microscopy data processing and model building (ie cryoSPARC, ChimeraX, Coot, Isolde, Phenix)
References
- Prof. Daniel Leahy, University of Texas at Austin, Department of Molecular Biosciences, Austin, Texas, USA, dleahy@austin.utexas.edu
- Prof. George Georgiou, University of Texas at Austin, Department of Chemical Engineering, Austin, Texas, USA, gg@che.utexas.edu
Publications
- Park, J#, Bartzoka, F#, et al. Features of the Serological IgG Repertoire Elicited by Egg-based, Cell based, or Recombinant HA Seasonal Influenza Vaccines. Under Revision in Lancet Microbe. # Indicates equal contribution
- Bartzoka, F, Gonzalez-Magaldi, M, Byrne, P.O, Callery, N.I, Hristova, K and Leahy D.J. Activity of EGFR transmembrane region variants indicates specific transmembrane dimers are not required for EGFR activity. Biochem. J. 2022, Dec, 479(24).
- Voss, W.N., Hou, Y.J., Johnson, N.V., Delidakis, G., Kim, J.E, Javanmardi, K., Horton, A.P., Bartzoka, F. et al. Prevalent, protective and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science 2021 May 4; eabg5268. doi: 10.1126/science.abg5268.
- Goike, J.#, Hsieh, C.L.#, Horton, A.#, Gardner, E.C.#, Zhou, L., Bartzoka, F. et al. SARS-COV-2 Omicron variants conformationally escape a rare quaternary antibody binding mode. Commun. Biol. 2023 10.1038/s42003-023-05649-6 # Indicates equal contribution
- Bartzoka, F., Venetsanou, K., Clonis, Y. Adenosine reagent-free detection by co-immobilization of adenosine deaminase and phenol red on an optical biostrip. Biotechnol. J. 2015, 10, 136-142.
- Qerqez, A, Bartzoka, F, Johnson, N.V. et al. Structural basis for the recognition of post-fusion hCMV gB by a non-neutralizing antibody. In preparation
Timeline
Ph.D. - Molecular Biosciences
University of Texas At Austin
M.Sc. - Systems Biology
Agricultural University of Athens, Greece
B.Sc. - Agricultural Biotechnology
Agricultural University of Athens, Greece
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