Frances Middleton-Davis
JHU Undergraduate
Baltimore,MD
Summary
Third-year JHU undergraduate with a passion for molecular biology and interest in working in the service industry. Previous experience as research and development intern at Cytoskeleton Inc., JHU Bloomberg School of Public Health, and JH Medical Institute as well as technical theater assistant in student-run clubs. Demonstrated proficiency in quick learning, attention to detail, adaptability, and time management, as displayed in leadership of database construction projects and publication of peer-reviewed research. Looking forward to learn and expand upon these skills in a service-industry setting.
Overview
4
4
years of professional experience
2
2
years of post-secondary education
Work History
Research Intern
Watanabe Lab, JH Medical Institute
02.2024 - Current
- Presented research at the Rising Stars Symposium hosted by JH Medical Institute.
- Conducted EM analysis and data entry in Excel, maintaining high levels of consistency and accuracy throughout.
- Assisted in multiple projects simultaneously, demonstrating multitasking and time management capabilities.
- Optimized research methodologies through continuous improvement efforts based on feedback from peers and supervisors.
DNA Clone Database Developer
Cytoskeleton Inc.
05.2023 - Current
- Developed universal platform for clone creation and documentation using SnapGene and Microsoft Excel.
- Wrote SOPs for the clone creator and QC to keep details consistent.
- Implemented physical storage system to help streamline clone identity verification.
- Presented database process to lab team, answering questions and incorporating feedback.
- Work as virtual consultant for database support.
Research Intern
Leung Lab, Bloomberg School Of Public Health
02.2023 - 04.2023
- Purified mutant FUS proteins from E. coli cells to be used in publication-ready condensate assay imaging.
- Trouble-shot bacterial culture conditions, resulting in an improved consistency in protein yield.
- Independently developed and optimized a native-gel assay to observe conformation of separate protein salt-elution peaks during FPLC purification.
- Analyzed results intended for quality control and discovered new, consistent pattern in protein conformation.
Technical Theater Assistant
Barnstormers
08.2022 - 12.2022
- Collaborated with technical production team to design and construct a set in accordance with club budget.
- Adapted to director suggestions whilst constructing the set.
- Quickly learned technical skills and taught these to others.
Research and Development Intern
Cytoskeleton Inc.
12.2020 - 08.2022
- Optimized existing lab protocol. Increased efficiency of cell electro-fusion from 10% to ~100% of well-positive hybridomas.
- Utilized ImageJ to perform descriptive and quantitative analysis of data.
- Prepared project-related reports, presentations and manuscripts.
- Conceived and executed on research questions.
- Research resulted in a peer-reviewed publication.
Protein Analyst
Foldit
12.2020 - 12.2021
- Analyzed digital protein structures to predict therapeutic efficacy.
- Optimized existent protein structure and synthesized novel proteins to fulfill unique biochemical roles.
Education
Bachelor of Science - Molecular And Cellular Biology
Johns Hopkins University
Baltimore, MD 08.2022 - Current
Skills
Collaboration
Communication
Time Management
Adaptability
Technical Profile
- Microsoft Excel
- Technical Writing
- Workshop Tools
- Theater Set Design
- Theater Lighting Design and Operation
Accomplishments
- Published high-quality protocols on the public database, protocols.io.
- Among the Top 100 protein analysts out of 240,000 professional-grade participants on Foldit.
Publications
Method for detecting acetylated PD-L1 in cell lysates by immunoprecipitation and western blot analysis
Middleton-Davis F, Davis A, Middleton K (2022) Method for detecting acetylated PD-L1 in cell lysates by immunoprecipitation and western blot analysis. PLOS ONE 17(7): e0268887.
https://doi.org/10.1371/journal.pone.0268887
Timeline
Research Intern
Watanabe Lab, JH Medical Institute
02.2024 - Current
DNA Clone Database Developer
Cytoskeleton Inc.
05.2023 - Current
Research Intern
Leung Lab, Bloomberg School Of Public Health
02.2023 - 04.2023
Technical Theater Assistant
Barnstormers
08.2022 - 12.2022
Bachelor of Science - Molecular And Cellular Biology
Johns Hopkins University
08.2022 - Current
Research and Development Intern
Cytoskeleton Inc.
12.2020 - 08.2022
Protein Analyst
Foldit
12.2020 - 12.2021
Similar Profiles
Jonathan LiJonathan Li
Intern at Jovanovic-Talisman Lab, Beckman Research Institute of City of Hope National Medical CenterIntern at Jovanovic-Talisman Lab, Beckman Research Institute of City of Hope National Medical Center
Darae KangDarae Kang
Researcher at Werth Lab- Perelman School of Medicine/Penn Institute for Immunology/ VA Medical CenterResearcher at Werth Lab- Perelman School of Medicine/Penn Institute for Immunology/ VA Medical Center
Patience ChulaPatience Chula
Laboratory Trainer & Mentor at Kenya Medical Research Institute (KEMRI HIV Lab- Nairobi & CIPDCR Alupe HIV EID Laboratory)Laboratory Trainer & Mentor at Kenya Medical Research Institute (KEMRI HIV Lab- Nairobi & CIPDCR Alupe HIV EID Laboratory)