FRANCISCO ZENO JR
Newberry,FL
Summary
Manufacturing Technician Highly-motivated and self-driven professional, offering 20 years of experience and expertise on processes toward growth and advancement within the pharmaceutical field. Strong background in conducting review of standard work instructions, standard operating procedures, and batch production records. Comprehensive knowledge of current GMPs.
Overview
24
24
years of professional experience
Work History
Downstream Manufacturing Specialist
Resilience
Alachua, FL
11.2021 - 01.2024
- Work in a clean room environment/following aseptic techniques in a BSC.
- Perform downstream activities including small scale chromatography using prepacked columns and columns that had to be manually packed following MBR and SOP's.
- Perform Viral filtration
- UF/DF operations.
- Perform autoclave duties and make set ups for process.
- Led a successful project with a team, it was completed, reviewed and submitted in a timely manner.
- Conducted walk through with QA and submitted tickets to Spiceworks to resolve findings.
Manufacturing Associates III
SEQIRUS
Holly Springs, NC
01.2015 - 09.2021
- Operate large scale chromatography equipment, ultra-filtration/dial-filtration and ultracentrifuge following manufacturing batch records and SOP’s
- Perform task in a clean room environment, sampling with aseptic techniques in a laminar flow hood
- Prepare buffers for production use and test filters with the Sartocheck Integrity testor
- Cip, Sip and pressure test tanks and pressure cans and maintain area logs and general housekeeping
- Perform SAP, GLIMS transactions and interface process automation using Allen Bradley system and Unicorn software
- Interact with other departments
- Support Validation and Qualification activities
- Cross trained in buffer charging to help assist our production needs.
Mab Manufacturing Technician II
BIOTEST PHARMACEUTICALS
Boca Raton, FL
01.2014 - 01.2015
- Maintain, operate and monitor a 2,000L Bioreactor in a clean room environment, under cGMP guidelines and aseptic techniques
- Perform equipment cleaning, CIP, SIP and pressure test of the bioreactor and filter housings
- Prepare media and buffers for production use including preparing set-ups and operation of the Girton Washer and Autoclave
- Operate Sartocheck filter integrity tester
- Obtain ampoules for vial thaw from cryo tank and cell expansion, cell feeding, harvest and cell count following batch record and standard operating procedures
- Sampling performed in a BSC following aseptic techniques
- Support other groups such as validation and process development
Manufacturing Purification Technician
Biotest
Boca Raton, FL
01.2012 - 01.2014
- Manufactured plasma through protein separation and performed other tasks involving Ultra-filtration, Dial-filtration, Chromatography and Nano-Filtration in a clean room environment following aseptic techniques
- Dispensed raw materials, prepare buffers and set-ups for production use, reviewed documentation and checked all calculations
- Followed cGMP, SOP and Manufacturing documentation, complied with safety requirements.
Monoclonal Antibody Production/HTLV Production
Abbott
Abbott Park
01.2009 - 01.2012
- Operated in a clean room environment (Class II) conducted under GMP guidelines/aseptic techniques to produce Monoclonal antibodies by hollow fiber for use in the manufacture of ADD Diagnostic Assays
- Monitored the production equipment/room conditions and systems and maintained proper documentation in batch record
- Manufactured Human T-Cell Lymphotropic Virus in a Biosafety Level III sterile environment both in tissue culture and purification areas, performing cell counts to determine cell growth/viability
- Performed cell splits per mbr, purified harvest in a continuous flow centrifuge and made media and buffers for production use
- Created, maintained, and promoted all quality systems and procedures in compliance to QC standards, obtaining ampoules from specific cell banks and scaled up cells in T-flasks and roller bottles
- Harvested cells, centrifuged, and pooled for downstream purification
- Performed daily cGMPs including equipment logs, chart recorders, cold rooms and incubators
Bead Grinding/Bead Coating
Abbott
Abbott Park, IL
01.2005 - 01.2009
- Performed bead grinding and bead coating with antibodies/solutions used in ADD diagnostic assays for the rare reagent
- Handled equipment troubleshooting, quarterly PMs and performed AMM and MES transactions
- Multi-tasked in both Bead Grinding and Coating to ensure product was finished on time while still retaining compliance
Biological Manufacturing Operator/Isolation
Abbott
North Chicago, IL
01.2000 - 01.2005
- Manufactured Abbokinase from human cells via tissue culture processes
- Operated on chromatography equipment, small to large scale columns, ultra-filtration/dial-filtration, fraction collector and final fill equipment
- Trained in the Abbokinase Pilot Plant for the re-launch of Abbokinase.
Education
Diploma - Computer Business
The College of Puerto Rico
Skills
- Cell Culture Techniques
- Aseptic Techniques
- Upstream
- Downstream
- CGMP's, SOP's and MBR's
- Chromatography/UF/DF/Viral filtration
- Aseptic Techniques
- SAP, LIMS, DeltaV, Unicorn and QAD
- Clean room environment
Timeline
Downstream Manufacturing Specialist
Resilience
11.2021 - 01.2024
Manufacturing Associates III
SEQIRUS
01.2015 - 09.2021
Mab Manufacturing Technician II
BIOTEST PHARMACEUTICALS
01.2014 - 01.2015
Manufacturing Purification Technician
Biotest
01.2012 - 01.2014
Monoclonal Antibody Production/HTLV Production
Abbott
01.2009 - 01.2012
Bead Grinding/Bead Coating
Abbott
01.2005 - 01.2009
Biological Manufacturing Operator/Isolation
Abbott
01.2000 - 01.2005
Diploma - Computer Business
The College of Puerto Rico