Gabriel Raimundo
Gainesville,FL
Summary
Expert in laboratory management, staff training, and advanced plant molecular genetics research. Proficient in ensuring laboratory safety, compliance with hazardous waste regulations, and developing standard operating procedures for experiments. Demonstrated ability in molecular biology techniques,with experience in major crops and model plants. Skilled in technical writing for grants and publications, contributing to high-impact scientific projects.
Overview
10
10
years of professional experience
Work History
Biological Scientist II
University of Florida
10.2025 - Current
- Selection and training of staff members and students
- Modification and implementation of research methods and scientific literature search to refine SOPs
- Conduct laboratory experiments, ensuring execution aligns with research goals
- Lab coordination and equipment maintenance
- Record and maintain detailed research procedures and results
- Analyze data using statistical models and adjust research designs based on findings
- Contribute to research papers, reports, and scholarly articles
- Engage in continuous learning and adjust to evolving research needs.Laboratory management: advising students and staff members, waste management, supervised daily operations ensuring compliance with safety and institutional guidelines.
- Optimization of nuclei extraction protocol for single cell samples from tissues of different crops: sorghum roots; maize and citrus leaves; broccoli flowers; lettuce, tomato, and blueberry calli; and citrus and strawberry fruits.
- Optimized lab workflows by coordinating equipment maintenance, service contracts and by organizing chemical inventory, seeds book, clone book, primer book, and lab consumables.
- Supported multiple concurrent projects, ensuring deadlines were met and resources allocated effectively.
Research Scholar
University of Florida
12.2023 - 10.2025
- Laboratory management: advising students and staff members, waste management, supervised daily operations ensuring compliance with safety and institutional guidelines.
- Optimization of nuclei extraction protocol for single cell samples from tissues of different crops: sorghum roots; maize and citrus leaves; broccoli flowers; lettuce, tomato, and blueberry calli; and citrus and strawberry fruits.
- Optimized lab workflows by coordinating equipment maintenance, service contracts and by organizing chemical inventory, seeds book, clone book, primer book, and lab consumables.
- Supported multiple concurrent projects, ensuring deadlines were met and resources allocated effectively.
Post-Master
Brazilian Agricultural Research Corporation (Embrapa)
09.2022 - 12.2023
- Execution of bioassays in greenhouses challenging maize against insects and phytopathogenic fungi
- Maize editing via CRISPR CAS9 system, maize transformation and breeding, maize phenotyping, and prospection of lineages resistant to biotic and abiotic stresses.
- Adapted SOPs and maintained organized lab documentation for CRISPR-edited plants.
MSc Student
Plant Molecular Biology Laboratory – BIOAGRO - Universidade Federal de Viçosa
, Brazil
08.2019 - 11.2021
- Development of research focused on NIG (NSP-Interacting GTPase), a potential proviral protein linked to recessive resistance against Begomovirures.
- Responsible for executing plant stable transformation, PCR, qRT-PCR, Western Blotting, yeast two-hybrid assay, transient expression of proteins in N. tabacum and benthamiana, nuclear fractionation assays, protein coimmunoprecipitation, gene cloning through Gateway system, BiFC, protein colocalization via confocal microscopy.
Undergraduate Student - Research Scholarship
Department of Biochemistry and Celular Biology - Universidade Federal de Viçosa
, Brazil
08.2017 - 07.2019
- Plant transformation, biolistics, PCR, RT-qPCR, Western Blotting, transient expression of proteins in tobacco and benthamiana, BiFC, protein colocalization via confocal microscopy, extraction, and quantification of plant hormones via chromatography.
Intern
Department of Biochemistry and Celular Biology - Universidade Federal de Viçosa
, Brazil
01.2017 - 02.2017
- Training in plant molecular biology techniques: cloning into expression vectors, plasmid DNA extraction, PCR, RNA extraction, bacteria transformation, yeast transformation, Agrobacterium tumefaciens-mediated plant transformation, yeast two-hybrid assay, subcellular localization of plant proteins through confocal microscopy.
Intern
Brazilian Agricultural Research Corporation (Embrapa)
, Brazil
01.2016 - 03.2016
- Characterization of antagonistic activity of Trichoderma spp. strains against Fusarium verticilioides responsible for fumonisins production isolated from maize and sorghum.
- Fungi DNA extraction and PCR.
Education
MSc Genetics and Breeding - Plant Molecular Genetics
Universidade Federal de Viçosa (UFV)
Brazil11.2021
Agronomist Engineer -
Universidade Federal de Viçosa (UFV)
Brazil07.2019
Environmental Technician -
Centro Federal de Educação Tecnológica de Minas Gerais (CEFET)
Brazil12.2013
Skills
- Laboratory management
- Team leadership
- Protocol optimization
- Laboratory safety protocols
- Writing reports
- In vitro assays
- In vivo studies
- Experimental design
- Gene expression analysis
- CRISPR-cas9 technology
- RNA interference techniques
- Data analysis
- Technical documentation
- Budget management
- Plant tissue culture
- DNA sequencing
- Sampling processes
Publications
- An improved nuclei isolation protocol from leaf tissue for single-cell transcriptomics., Madrid G, Raimundo GAS, Reyes FA, Picoli EAdT, Resende Jr. MFR, et al., PLOS ONE, 09/01/25, https://doi.org/10.1371/journal.pone.0302118
- Begomovirus-host protein-protein interactions in intracellular virus movement., Silva FA; Raimundo GAS; Fontes EB., Geminivirus: Detection, Diagnosis and Management, 2022, Academic Press
- Roteiros de Práticas de Laboratório de Bioquímica., Viana, PA.; Raimundo, GAS.; Melo, Bruno P; Siqueira, RS., 2022, Editora UFV
- Geminiviral Triggers and Suppressors of Plant Antiviral Immunity., Teixeira, RM., Ferreira MA, Raimundo GAS, Fontes., Microorganisms, 2021
- A Begomovirus Nuclear Shuttle Protein-Interacting Immune Hub., Martins LGC, Raimundo GAS, Ribeiro NGA, Silva JCF, Euclydes NC, Loriato VAP, et al., Front Plant Sci., 2020
- Virus perception at the cell surface: revisiting the roles of receptor-like kinases as viral pattern recognition receptors., Teixeira RM, Ferreira MA, Raimundo GAS, Loriato VAP, Reis PAB, Fontes EPB., Mol Plant Pathol., 2019
Languages
- Portuguese, Native
- English, Fluent
- Spanish, Intermediate
- Chinese (Mandarin), Elementary
Complementary Information
- Experience as a Teacher Assistant at University of Florida (01/01/25 - 04/30/25).
- Experience as a Teacher Assistant at Universidade Federal de Viçosa in various subjects.
- Experience with R software.
- 10 publications in conferences.
Timeline
Biological Scientist II
University of Florida
10.2025 - Current
Research Scholar
University of Florida
12.2023 - 10.2025
Post-Master
Brazilian Agricultural Research Corporation (Embrapa)
09.2022 - 12.2023
MSc Student
Plant Molecular Biology Laboratory – BIOAGRO - Universidade Federal de Viçosa
08.2019 - 11.2021
Undergraduate Student - Research Scholarship
Department of Biochemistry and Celular Biology - Universidade Federal de Viçosa
08.2017 - 07.2019
Intern
Department of Biochemistry and Celular Biology - Universidade Federal de Viçosa
01.2017 - 02.2017
Intern
Brazilian Agricultural Research Corporation (Embrapa)
01.2016 - 03.2016
MSc Genetics and Breeding - Plant Molecular Genetics
Universidade Federal de Viçosa (UFV)
Agronomist Engineer -
Universidade Federal de Viçosa (UFV)
Environmental Technician -
Centro Federal de Educação Tecnológica de Minas Gerais (CEFET)