Summary
Overview
Work History
Education
Skills
Websites
Supervision And Mentoring
Educational Teaching Training
Scholarships And Awards
Google Scholar
Research Publications
Languages
Timeline
Generic

HARI P DULAL

Ithaca,NY

Summary

With a strong background in research principles and a deep interest in scientific discovery, I am enthusiastic about learning and growing within a research environment. Possessing expertise in data collection and analysis, as well as proficiency in laboratory techniques, I can make valuable contributions to research projects. I am prepared to apply and enhance my analytical and research skills in the desired role, leveraging my genuine passion for scientific exploration.

Overview

18
18
years of professional experience

Work History

Research Associate

Cornell University
04.2023 - Current

I am working to develop novel keratinolytic enzymes from several microbes to utilize them for feather hydrolysis so that the tons of waste feathers can be turned into animal protein food.

Postdoctoral Associate

Cornell University
05.2019 - 04.2023

I studied the molecular mechanism of Typhoid pathogenesis and development of more potent vaccines and therapeutics against Typhoid fever. I used the Alpaca derived single domain antibodies to test their capacity to neutralize the toxin in cell lines and mice. The single domain antibodies successfully neutralized typhoid toxin saving mice proving effectiveness of neutralizing the toxin.

Associate Professor

Chitwan Medical College
10.2016 - 04.2019

I taught clinical biochemistry and molecular biology to undergraduate and graduate students in this medical college and supervised undergraduate thesis work as well as clinical laboratory lab.

Assistant Professor

Chitwan Medical College
03.2011 - 09.2013

I taught clinical biochemistry and molecular biology courses to undergraduate and graduate students.

Assistant Professor

Jiujiang University
09.2009 - 08.2010

I handled the department of biochemistry for curriculum design, teaching, evaluation and administration for biochemistry course for international medical undergraduates. I taught whole theory and practical courses of biochemistry for 51 students.

Lecturer

Nepal Medical College
08.2007 - 09.2009

I taught clinical biochemistry and molecular biology courses to undergraduate and graduate students.

Education

PhD - Life Science and Technology

Tokyo Medical And Dental University/RIKEN
Tokyo, Japan
09.2016

Master of Science - Medical Biochemistry

B.P. Koirala Institute of Health Sciences
Dharan, Nepal
07.2006

Bachelor of Science - Microbiology

Tribhuvan University
Dharan, Nepal
06.2002

Skills

  • Primers design
  • Conventional PCR
  • Cloning by Gibson Assembly
  • Restriction digestion and ligation
  • Site-directed mutagenesis
  • Real-time PCR
  • Generation of gene deletion knockouts
  • Gene complementation
  • Recombinant protein expression in E coli, Yeast, Fungi
  • Recombinant protein purification
  • Fast Protein Liquid Chromatography (FPLC)
  • Ion-exchange chromatography
  • Affinity purification
  • Size exclusion chromatography
  • In-vitro characterization of proteins
  • UV/Fluorescence spectrometry
  • Electrophoretic mobility shift assay (EMSA)
  • Gel electrophoresis
  • Isothermal Titration Calorimetry (ITC)
  • Dynamic Light Scattering (DLS)
  • Surface Plasmon Resonance (SPR)
  • Protein crystallization
  • Bacterial culture, Isolation, Sterilization
  • Microscopy
  • Quality control
  • Nuclear Magnetic Resonance (NMR) sample preparation
  • Proton NMR
  • HSQC measurement
  • Enzyme-linked Immunosorbent Assay (ELISA)
  • Western Blotting
  • Flow cytometry (FACS)
  • Mammalian cell line culture
  • Cell intoxication
  • Cell viability assay
  • Transfection
  • Mice handling
  • Colony maintenance
  • Intraperitoneal injection
  • Retro-orbital injection
  • Motor function assays
  • Clinical biochemistry laboratory tests
  • Biological data analysis tools
  • SnapGene
  • GraphPad Prism
  • FlowJo
  • ImageJ
  • Report writing
  • Presentation
  • Optimization
  • Troubleshooting
  • Scientific writing
  • Lab operations
  • Student research guidance
  • Lab management
  • Multitasking
  • MS office
  • Research presentation

Supervision And Mentoring

  • Principal guide for undergraduate thesis work, Correlation of fasting plasma glucose and glycated hemoglobin with serum electrolytes in type 2 diabetic patients attending at Chitwan Medical College Teaching Hospital, Bachelor of Science in Medical Lab Technology, 2016-2017
  • Clinical lab supervisor, In respective medical college diagnostic labs mentioned in professional experience section.
  • Mentoring and assisting, Undergraduate and graduate students research work in Cornell University.

Educational Teaching Training

  • Advancing Learning Through Evidence-Based Teaching, The Center for the Integration of Research Teaching and Learning (CIRTL), Training completed with distinction
  • Medical education faculty development, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal
  • Modern trends in medical education, KUMJ & KMC, 01/30/09, 02/01/09, Kathmandu, Nepal
  • Comprehensive review course on immunology, Patan Academy of Health Sciences, 08/07/08, 08/08/08, Lalitpur, Nepal
  • Molecular aspects of atherosclerosis and related metabolic disorder, Department of Biochemistry, All India Institute of Medical Sciences, 10/24/05, New Delhi, India

Scholarships And Awards

  • CHMID travel award, ASM microbe 2022, Washington DC, USA
  • Nepal Education Excellency Award, Ministry of Education, Government of Nepal
  • The international symposium of Glycobiology 2017 travel award, 11/16/17, 11/17/17, Japan
  • International Program Associate, PhD studentship scholarship, RIKEN, Japan

Google Scholar

https://scholar.google.com/citations?user=QRs9F0cAAAAJ

Research Publications

  • Hydrolysis of keratins by novel keratinase enzymes, manuscript in preparation
  • Neutralization of typhoid toxin by alpaca derived, singe domain antibodies targeting the PltB and CdtB subunits, Infect Immun, 2022, 90, 2, e00515-21
  • Enteric fever diagnosis: current challenges and future directions, Pathogens, 2021, 10, 4, 410
  • The core fucose on an IgG antibody is the endogenous ligand of Dectin-1, Angew. Int. Ed., 2019, 58, 2-8
  • Association between Lys198Asn polymorphism of endothelin‐1 gene and ischemic stroke: A meta‐analysis, Brain. Behav., 2019, 9, e01424
  • Β-glucan-induced cooperative oligomerization of Dectin-1 C-type lectin-like domain, Glycobiology, 2018, 28, 612-623
  • Enhancement of solubility and yield of a β-glucan receptor Dectin-1 C-type lectin-like domain in Escherichia coli with a solubility-enhancement tag, Protein Expr. Purif., 2016, 123, 97-104
  • Status of Iron, Oxidant and Antioxidants in chronic type 2 diabetes mellitus patients, Nepal Med. Coll. J., 2014, 16, 54-57
  • Study the effects of hyperthyroidism on oxidative stress in Nepalese population, Biomedicine, 2009, 29, 176-179
  • Oxidative stress in type 2 diabetes mellitus-A hospital-based study at B.P. Koirala Institute of Health Sciences, Dharan, Nepal, Biomedicine, 2005, 25, 17-21

Languages

English
Full Professional
Hindi
Full Professional
Nepali
Native or Bilingual
Japanese
Elementary

Timeline

Research Associate

Cornell University
04.2023 - Current

Postdoctoral Associate

Cornell University
05.2019 - 04.2023

Associate Professor

Chitwan Medical College
10.2016 - 04.2019

Assistant Professor

Chitwan Medical College
03.2011 - 09.2013

Assistant Professor

Jiujiang University
09.2009 - 08.2010

Lecturer

Nepal Medical College
08.2007 - 09.2009

PhD - Life Science and Technology

Tokyo Medical And Dental University/RIKEN

Master of Science - Medical Biochemistry

B.P. Koirala Institute of Health Sciences

Bachelor of Science - Microbiology

Tribhuvan University
HARI P DULAL