Summary
Overview
Work History
Education
Skills
SELECTED PUBLICATIONS
ADDITIONAL INFORMATION
Languages
Timeline
Generic

HEMAVATHY HARIKRISHNAN

Indianapolis,IN

Summary

Ph.D. Molecular biologist with 12+ years of research experience specializing in gene therapy, viral vector biology, and quantitative analytical method development. Deep expertise in molecular biology techniques applied to in vitro and in vivo models, gene editing, and disease characterization. Published researcher with a proven ability to independently design and execute complex experiments, generate high-quality data, and communicate findings clearly across multidisciplinary teams.

Overview

7
7
years of professional experience

Work History

Assistant Research Professor

Indiana University School of Medicine, Department of Pediatrics
Indianapolis, IN
05.2026 - Current
  • Designed and executed qPCR-based quantitative assays to evaluate CRISPR/Cas9 gene-editing efficiency and therapeutic strategies in human and mouse stem cell models; directly analogous to genome titration and residual DNA testing in viral vector programs
  • Developed and optimized transfection-based workflows (lipid and electroporation) for lentiviral/AAV constructs, maintaining rigorous experimental controls and reproducibility standards
  • Conducted high-throughput screening to identify druggable targets in Fanconi anemia disease models, applying RT-qPCR and functional cell-based readouts across multiple assay formats
  • Maintained meticulous electronic laboratory records consistent with GLP documentation practices; contributed to manuscript preparation and regulatory-style technical summaries
  • Promoted from Postdoctoral Fellow (Aug 2024 – Apr 2026)

Postdoctoral Fellow — Ophthalmology & Disease Biology

Indiana University School of Medicine, Department of Ophthalmology
Indianapolis, IN
08.2020 - 04.2024
  • Developed and qualified cell-based assays to study protein function relevant to Usher syndrome; applied ELISA, immunoprecipitation, Western blot, and qPCR across study matrices
  • Generated and validated CRISPR/Cas9 knockout cell lines for functional characterization; quantified genome-editing outcomes using molecular biology techniques including gel electrophoresis and restriction analysis
  • Performed confocal microscopy, ICC/IHC, and high-content imaging to analyze cellular phenotypes; supported cross-functional projects with analytical data and technical reports
  • Contributed proteomics data (SILAC, LC-MS/MS) as orthogonal characterization approach for target identification

Postdoctoral Fellow — Protein Target Characterization

Case Western Reserve University
USA
08.2019 - 08.2020
  • Designed molecular and cell-based assays to validate novel protein targets; applied quantitative proteomics (SILAC, LC-MS/MS) to prioritize candidate pathways relevant to disease biology
  • Executed gel electrophoresis, blotting, and restriction enzyme analysis for product characterization

Education

Ph.D. - Pharmacology

National University of Malaysia
Malaysia
01-2018

Master's - Medical Microbiology

University Science Malaysia
Malaysia
01-2014

B.Sc. (Hons) - Biotechnology

AIMST University
Malaysia
01-2010

Skills

  • Cell Biology & Assays: Mammalian and primary cell culture, stable cell line development transfection (lipid/electroporation), stable cell line development, assay development & optimization, lentiviral/AAV transduction & titration
  • Molecular Biology: CRISPR/Cas9 gene editing, RT-qPCR, RNA/DNA extraction, cloning, siRNA knockdown, ELISA, Western blot, proteomics (SILAC, LC-MS/MS)
  • Imaging & Cytometry: Confocal microscopy, ICC/IHC, flow cytometry
  • In Vivo: Mouse models of bone marrow failure & retinal disease — handling, dosing, tissue collection & processing
  • Bioinformatics & Data: GraphPad Prism, ImageJ, basic data analysis & visualization
  • Scientific Communication: Technical writing, manuscript preparation, method development reports, presentations to multidisciplinary audiences
  • Mentoring students

SELECTED PUBLICATIONS

  • Harikrishnan H et al. CRISPR/Cas9-mediated homology-directed repair rescues induced bone marrow failure in Fanconi anemia (FANCC) mice. Molecular Therapy Nucleic Acids, In Press, 2026.
  • Takita S., Harikrishnan H. et al. Transcriptional downregulation of rhodopsin is associated with desensitization of rods to light-induced damage in retinitis pigmentosa. Human Molecular Genetics, 2025.
  • (Full list available upon request)

ADDITIONAL INFORMATION

  • Authorized to work in the United States; — no employer sponsorship required
  • Effective communicator of complex technical data to multidisciplinary project teams and stakeholders
  • Proven ability to manage multiple concurrent priorities in fast-paced, collaborative research environments

Languages

English
Full Professional

Timeline

Assistant Research Professor

Indiana University School of Medicine, Department of Pediatrics
05.2026 - Current

Postdoctoral Fellow — Ophthalmology & Disease Biology

Indiana University School of Medicine, Department of Ophthalmology
08.2020 - 04.2024

Postdoctoral Fellow — Protein Target Characterization

Case Western Reserve University
08.2019 - 08.2020

Master's - Medical Microbiology

University Science Malaysia

B.Sc. (Hons) - Biotechnology

AIMST University

Ph.D. - Pharmacology

National University of Malaysia