Hilda Fregene
San Diego,CA
Overview
9
9
years of professional experience
Work History
Data Entry/ Laboratory Technician
San Diego Reference Laboratory
09.2024 - Current
- Efficiently handle and process a high volume of laboratory specimens, ensuring accurate labeling, documentation and preparation for forensic toxicology analysis
- Conduct toxicological drug screening and confirmation using Gas Chromatography- Mass Spectrometry (GC-MS), adhering to established protocols and quality standards
- Adhere to strict chain-of-custody protocols to maintain the integrity of samples and comply with forensic industry standards
- Collaborate with laboratory staff to prioritize workflow and meet stringent deadlines for sample testing and reporting
- Perform data entry and verification of sample information into laboratory information management system (LIMS) with precision and attention to detail
Laboratory Processor
Olgam Life
02.2024 - 08.2024
- Retrieved 500+ VMT, NAT, SPE and ABY plasma samples independently daily for further testing and shipment
- Implemented and adhered to internal Standard Operating procedures (SOPs), ensuring the highest standards of quality assurance and quality control (QA/QC) in laboratory procedures
- Developed a system ensuring all supplies were available on time, and improving inventory management process by over 65%
Laboratory technician
KAM consultants
01.2023 - 02.2024
- Prepared and analyzed over 1,000 air and bulk asbestos samples daily for Polarized Light Microscopy (PLM), Phase Contrast Microscopy (PCM), and Transmission Electron Microscopy (TEM) analyses
- Authored, reviewed, and revised 100+ comprehensive reports daily, chain of custody documents and lab forms showcasing adherence to protocols
- Established inventory for all laboratory supplies and chemicals
- Processed sample preparation and collection daily in a 10 - member team
Graduate Research Assistant
Pace University
02.2022 - 12.2023
- Developed and optimized immunoassays (ELISA, flow cytometry, Western blot, and multiplex bead-based assays) to support ongoing research projects
- Performed protein purification (affinity chromatography, size-exclusion chromatography, and ion exchange chromatography) to ensure high-quality protein yields for experimental use
- Performed HPLC, GC, LC-MS, and NMR analyses to characterize compounds, ensuring high purity and structural confirmation
- Executed analytical assays, including UV-Vis spectroscopy, FTIR, and fluorescence-based techniques, to assess chemical and biochemical interactions
- Presented findings in cross-functional team meetings, contributing to data-driven decision-making for R&D projects
Graduate Student
Pace University
02.2022 - 12.2023
- Detected trace organic explosive analogs using reverse phase HPLC and arson accelerant detection using GC-MS
- Processed drug samples & organic compound assays and interpreting spectrum using Infrared Spectroscopy (IR), Attenuated total reflectance (ATR), Fourier transform infrared spectroscopy (FTIR), NMR, Mass Spectrometry and Raman Spectroscopy
- Analyzed trace evidence such as hairs, fibers & glass using procedures with standard microscopy and polarized light microscopy (PLM)
- Conducted DNA extraction, PCR, qPCR and capillary electrophoresis with fluorescent markers
Undergraduate researcher
Babcock University
04.2016 - 06.2021
- Performed neurotransmitter assay, immunohistochemistry analysis, histological testing and enzyme assay testing the biochemistry Wistar rats used for various studies
- Researched and demonstrated data of the neurodegenerative effects of 4G radiofrequency and the mild ameliorative effects of omega-3 fish oil on the prefrontal cortex of juvenile Wistar rats
- Managed a 10 - member cross functional (sample processing, sample collection, sample preservation) scientific team towards the analysis of the prefrontal cortex neurodegenerative conditions
- Utilized Electronic data capture (EDC) like Medidata rave for study digitization
Education
Master of Forensic science - Forensic science
Pace University
12.2023
Bachelor (Hons) - Anatomy
Babcock University
06.2019
Skills
- Chromatography
- Functional genomics
- Microscopic Techniques
- Mass Spectrometry
- Capillary Electrophoresis
- Population Genetics
- HPLC
- UV-Vis
- DNA sequencing
- NGS
- DNA extraction
- Graph pad prism
- MATLAB
Timeline
Data Entry/ Laboratory Technician
San Diego Reference Laboratory
09.2024 - Current
Laboratory Processor
Olgam Life
02.2024 - 08.2024
Laboratory technician
KAM consultants
01.2023 - 02.2024
Graduate Research Assistant
Pace University
02.2022 - 12.2023
Graduate Student
Pace University
02.2022 - 12.2023
Undergraduate researcher
Babcock University
04.2016 - 06.2021
Master of Forensic science - Forensic science
Pace University
Bachelor (Hons) - Anatomy
Babcock University
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