Holden Prymas
New Canaan,CT
Summary
Driven by deep curiosity for scientific research, and a desire to interrogate all aspects of Drosophila Melanogaster. A student investigator with the StanX research program at Lawrenceville, where I collaborated on a research team and mastered a multitude of fly lab skills. Eager to continue greater investigations and provide meaningful results that have real world application.
Overview
1
1
year of professional experience
Work History
Student Researcher
Lawrenceville School/Stanford School of Medicine
09.2024 - 05.2025
- In collaboration with the Seung Kim Lab at the Stanford School of Medicine, I developed novel transgenic lines of fruit fly to model human disease as part of the StanX program.
- Created and successfully executed a crossing scheme to move the SX4 p-element from the X chromosome to chromosomes 2 or 3.
- Confirmed the p-element location via iPCR sequencing and primer design.
- Dissected larvae and preformed immunostaining to confirm the activity pattern of the transgene.
- Created SX40 and SX9, novel driver lines I created which are now apart of the Bloomington stock for researchers use.
- SX40 trapped an enhancer on the 2nd chromosome near gene CG5853. However, has a separate expression pattern from the gene being more associated brain lobe, VNC, and Trachea.
- SX5 trapped an enhancer on the 2nd chromosome associated with the gene Gbs-70E. This gene is involved in the regulation of glycogen metabolism and could be used to study alcohol use disorder
- Presented findings to a group of 60+ students and faculty at an International Student Conference.
Bakery Clerk
Flour Salt Water Bread
06.2025 - Current
- Assisted customers with product selection and inquiries, enhancing shopping experience.
- Maintained cleanliness and organization of bakery display areas, ensuring compliance with health standards.
- Collaborated with team members to restock inventory, optimizing product availability on shelves.
Education
Bachelor of Science - Biology
Boston College
Chestnut Hill, MA05-2029
High School Diploma -
The Lawrenceville School
Lawrenceville, NJ05.2025
Skills
- PCR/iPCR
- Larval dissection
- Flipping vials
- Fly husbandry
- Aspiration
- Antibody staining
- Crossing scheme derivation
- Sequence analysis
- Primer design
- Navigating FlyBase
- Interacting with primary literature
Timeline
Bakery Clerk
Flour Salt Water Bread
06.2025 - Current
Student Researcher
Lawrenceville School/Stanford School of Medicine
09.2024 - 05.2025
Bachelor of Science - Biology
Boston College
High School Diploma -
The Lawrenceville School
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