Hong Yu
San Antonio,TX
Summary
Dynamic Postdoctoral Researcher with extensive detailed research protocols and experiment experience. I received interdisciplinary training in metabolic disease pathophysiology, glucose and lipid metabolism regulation and molecular biology. I am passionate about developing new biochemical assays and pursuing the role of glucose and lipid metabolism in metabolic diseases and associated cancer using multi-omics approach.
Overview
2
2
years of professional experience
Work History
Postdoctoral Researcher
University Of Texas Health Science Center At San Antonio
San Antonio, TX
05.2022 - Current
- I received my bachelor's degree in medicine, then went on for a
joint multidisciplinary Ph.D. training in cancer biology at the Beijing University of Chinese Medicine, Cancer Hospital Chinese Academy of Medical Sciences, and Chinese Academy of Sciences. - I specializes in CRISPR-cas9-mediated knockout and knockin techniques, oxidative stress, signaling transduction, and protein biochemistry. I am also training in metabolic disease pathophysiology, glucose and lipid metabolism regulation and molecular biology.
- Currently, I am passionate about developing new biochemical assays and pursuing the role of disrupted energy homeostasis and oxidative stress in NASH and liver cancer; glucose and lipid metabolism in metabolic diseases and associated cancer using multi-omics approach.
Education
POSTDOCTORAL FELLOW - Molecular Metabolism and Metabolic Disease
The UT Health Science Center At San Antonio
San Antonio, TX05-2022
Ph.D. - Cancer Therapy
Beijing University of Chinese Medicine
Beijing, China12-2021
Skills
Molecular biology skills
- CRISPR-cas9 (Lentiviral infection; RNP)
- Construct Knockin/Knockdown/Point mutation cell lines
- Construction varius AAV and lentivirus
- Gene fusion in CAR-T/CAR-M cells
- Isolation of primary hepatocytes and endothelial cells from humans and mice
- ELISA (including protocol design)
- Seahorse assay
- Cell cycle, migration, phagocytosis, proliferation, apoptosis, adhesion assays
- Immunofluorescence, immunohistochemistry, immunoblotting
- Lipid detection in cells (Intracellular cholesterol and triglycerides/Oil red strain/ Immunofluorescence/ QPCR)
- Imaging of spindle fibers, centrioles and centrioles in living cells
- Detect intracellular redox status (ROS level, JC-1 strain)
- RNA-seq (RNA extraction, RT- polyA RNA , synthesis double strand DNA , adding barcode, library quantification, pool samples for sequencing) and data analysis
- Single-cell RNA sequencing of mouse endothelial cells (sample preparation and data analysis)
- ChIP-seq sample preparation and data analysis
Mouse experiment skills
- Construct tissue-specific knockout gene mice using Cas9-expressing mice
- Combined knockout or overexpression of multiple genes in mice by Cre-Lox recombination technology
- Mouse aorta isolation and staining
- Construct orthotopic tumors model in the liver and spleen
- Mouse Ultrasound and IVIS Imaging
- Preparation and cryosectioning of mouse tissues, immunofluorescence, image acquisition
- Mouse intraperitoneal injection, subcutaneous injection, tail vein injection, subcutaneous tumor formation
- Mice Serum glucose, Glucose tolerance test, Insulin tolerance test
- Mouse metabolic flux analysis
Character
- Innovation and Creativity
- Team Collaboration
- Excellent Communication
- Time Management
- Analytical Thinking
- Critical thinking proficiency
- Effective Communication
- Problem-solving abilities
- Decision-Making
- Interpersonal Skills
- Adaptability and Flexibility
- Reliability
- Organizational Skills
- Self Motivation
Affiliations
1. Hong Yu#, Hong-Ying Gao#, Hua Guo, Gui-Zhen Wang, Yi-Qing Yang, Li-Jun Liang, Qun Zhao, Da-Wei Xie, Yu Rao*, Guang-Biao Zhou*，Upregulation of wild-type p53 by small molecule-induced elevation of NQO1 in non-small cell lung cancer cells，Acta Pharmacologica Sinica. 2021.
2. Liang, Li-Jun#; Yang, Fu-Ying#; Wang, Di#; Zhang, Yan-Fei#; Yu, Hong; Wang, Zheng; Sun, Bei-Bei; Liu, Yu-Tao; Wang, Gui-Zhen; Zhou, Guang-Biao*，CIP2A induces PKM2 tetramer formation and oxidative phosphorylation in non-small cell lung cancer. Cell Discovery. 2024.
3. Zhao, Peng#; Sun, Xiaoli; Liao, Zhongji; Yu, Hong; Li, Dan; Shen, Zeyang; Glass, Christopher K; Witztum, Joseph L; Saltiel, Alan R*，The TBK1/IKKε inhibitor amlexanox improves dyslipidemia and prevents atherosclerosis. JCI insight. 2022.
4. Guizhen Wang#, Qun Zhao, Fan Liang, Chen Zhang, Hui Zhang, Jun Wang，Zhenyin Chen, Ran Wu, Hong Yu, Beibei Sun, Hua Guo, Ruie Feng, Kaifeng Xu, Guangbiao Zhou*，Degradation of SARS-CoV-2 receptor ACE2 by the E3 ubiquitin ligase Skp2 in lung epithelial cells. Front Med. 2021.
5. Zhao XC#, Wang GZ#, Wen ZS#, Zhou YC#, Zhao XC, Wang GZ, Wen ZS, Zhou YC, Hu Q, Zhang B, Qu LW, Gao SH, Liu J, Ma L, Zhang YF, Zhang C, Yu H, Zhang DL, Wang M, Wang CL, Huang YC, Liu ZH, Zhao Y, Chen L, Zhou GB*. Systematic identification of CDC34 that functions to stabilize EGFR and promote lung carcinogenesis. EBioMedicine. 2020; 53: 102689.
6. Zhao XC#, Yongchun Z, Qian H, Sanhui G, Jie L, Hong Y, Yanfei Z, Guizhen W, Yunchao H, Guangbiao Z*. Identification of a potential tumor suppressor gene, UBL3, in non-small cell lung cancer. Cancer Biol Med. 2020; 17: 76-87.
7. Wang M#, Yu H, Wu R, Chen ZY, Hu Q, Zhang YF, Gao SH, Zhou GB*. Autophagy inhibition enhances the inhibitory effects of ursolic acid on lung cancer cells. Int J Mol Med. 2020; 46: 1816-1826.
8. Wang GZ#, Zhang L#, Zhao XC#, Gao SH#, Qu LW, Yu H, Fang WF, Zhou YC, Liang F, Zhang C, Huang YC, Liu Z, Fu YX, Zhou GB*. The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat Commun. 2019; 10: 1125.
9. Da-Lin Zhang#, Li-Wei Qu#, Liang Ma, Yong-Chun Zhou, Gui-Zhen Wang, Xin-Chun Zhao, Chen Zhang, Yan-Fei Zhang , Min Wang, Mei-Ying Zhang, Hong Yu , Bei-Bei Sun, San-Hui Gao, Xin Cheng, Ming-Zhou, Yun-Chao Huang, Guang-Biao Zhou*, Genome-wide identification of transcription factors that are critical to non-small cell lung cancer[J]. Cancer Lett, 2018, 434: 132-143.
Timeline
Postdoctoral Researcher
University Of Texas Health Science Center At San Antonio
05.2022 - Current
POSTDOCTORAL FELLOW - Molecular Metabolism and Metabolic Disease
The UT Health Science Center At San Antonio
Ph.D. - Cancer Therapy
Beijing University of Chinese Medicine
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