Summary
Overview
Work History
Education
Skills
Affiliations
Workshop and Seminars
References
Timeline
Generic

HRITHIK PAHUJA

Bozeman,MT

Summary

Dynamic and dedicated professional with a Master of Science in Plant Pathology and a solid agricultural background rooted in potato seed production. Possessing extensive expertise gained through work in diverse molecular biology labs, specializing in molecular techniques relevant to crop improvement. Driven by a passion for molecular techniques, I hope to foster innovation and spearhead advancements in potato cultivation within a dynamic potato lab.

Overview

5
5
years of professional experience

Work History

Graduate Teaching Assistant

Montana State University Bozeman
Bozeman, MT
01.2023 - Current
  • Delivered lab lectures to undergraduate classes.
  • Evaluated student performance through tests, quizzes, presentations, projects, or other assessments.
  • Involved in departmental activities such as curriculum development, faculty meetings, advising sessions.

Summer Intern Researcher

Punjab Agricultural University
Ludhiana, PB
05.2019 - 07.2024
  • Supported other researchers in CRISPR-mediated genome editing in Maize to develop haploid inducer stock.

Summer Intern

C&B Operations
Billings, MT
06.2023 - 08.2023
  • Provided advice to key clients on operational processes, business development initiatives, organizational changes, and other areas of improvement using John Deere Operation Center.

Graduate Research Assistant

Montana State University Bozeman
Bozeman, MT
01.2022 - 12.2022
  • H Pahuja, S Bansal, W Dutta, S Kukal, G Jha (2024) A Global Meta-Analysis on Delineating Zones for Variable Rate Irrigation in Solanaceous crops. (In preparation)
  • G Jha, S Ghatrehsamani, H Pahuja (2024) Review Article: Application of Wireless Sensor Network in Dryland Agriculture. (In preparation)
  • Optimization of Winter Wheat Growth in Greenhouse Conditions. (May 2022 – Dec 2022)
  • Investigating the role of callose deposition as a mechanism of resistance to Potato Virus Y in potato plants. (Jan 2022 – May 2022)

Research Project Trainee

Punjab Agricultural University
Luhdiana, PB
08.2020 - 08.2021
  • Allele mining of ZmIBH-1 gene that regulates plant architecture in Maize.
  • Inducing variation in ZmPLA1 gene through CRISPR/CAS9 RNP complex.
  • Crossing early varieties with late varieties of PEA Plant to generate F1.

Education

Bachelor of Science - Biotechnology

Punjab Agricultural University
Ludhiana, PB
08-2021

Master of Science - Plant Pathology

Montana State University - Bozeman
Bozeman, MT

Skills

Lab Skills:

  • RNA Isolations (Trizol Methol)
  • cDNA synthesis (Reverse Transcriptase)
  • Quantitative Polymerase Chain Reaction
  • Topo-TA cloning
  • Plasmid Purification
  • gRNA synthesis for CRISPR-based gene editing
  • Transformation (Using Biolistic, Heat shock treatment)
  • Protein extraction
  • Media Preparation
  • Tissue culture
  • Embryo Culture
  • Virus inoculum preparation
  • Polyphenol oxidase analysis

Field Skills:

  • Transplanting
  • Post-sowing management
  • Selection/Screening
  • Emasculation
  • Crossings
  • Spore isolation (Stem Rust collection from the wheat)
  • Virus Inoculation

Software Skills:

  • Microsoft office
  • R-Studio (Beginner level)
  • Gel images analysis in ImageJ
  • Gene sequence alignment in Geneious, Clustal Omega
  • Gene Mapping and generating a linkage Map using MapMaker 30

Affiliations

  • Represented PAU in the North Zone Inter-University football tournament. (2019)
  • Winner of Inter-College football tournament held at PAU. (2019)
  • Participated in a 200m sprint race at the district level at Sainik School, Kapurthala, Punjab, India. (2014)

Workshop and Seminars

  • Attended the 107th annual Potato Association of America meeting in Charlottetown, Prince Edward Island, Canada, from July 23, 2023, to July 27, 2023.
  • Attended the 106th annual Potato Association of America meeting in Missoula, Montana, USA, from July 17, 2022, to July 21, 2022.
  • Poster presentation on ‘Speed Vernalization in winter wheat of Montana’ at Post Farm on ‘Field Day (July 07, 2022)’ organized by Montana State University, Bozeman, USA.
  • Participated in “Hands-on laboratory course on CRISPR-Cas Gene Editing.” Jointly organized by Shri Guru Gobind Singh Tricentenary (SGT) University, Gurugram, and Alliance of Bioversity International and CIAT, New Delhi, on March 23-27, 2021.
  • Participated in E-Quiz on “Food Nutrition and Agriculture” on world food day organized by Junagarh Agriculture University, Gujrat, IN.
  • Participated in E-Quiz on “Biotechnology in Nature Conservation and Agriculture” organized by Junagarh Agriculture University, Gujrat, IN.
  • Represented presentation on “Hemizygous mutations in potato” at the School of Agricultural Biotechnology, PAU, LDH, IN.
  • Workshop cum training on Techniques in Molecular Biology conducted by the School of Agricultural Biotechnology, PAU, LDH in collaboration with wet-lab championship and Shaastra (IIT Madras) on 18th & 19th Oct 2019.

References

  • Dr. Daniel Colman: Assistant Research Professor, Department of Microbiology & Cell Biology, Montana State University, Mailto: Daniel.colman@montana.edu
  • Dr. Gaurav Jha: Assistant Professor, Department of Agronomy, Kansas State University, Manhattan, Kansas, Mailto: gjha@ksu.edu
  • Dr. Suchismita Mondal: Assistant Professor, Plant Sciences/Plant Pathology, Montana State University, Bozeman-MT, Mailto: suchismita.mondal@montana.edu
  • Dr. Priti Sharma: Biotechnologist, School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-PB, Mailto: pritisharma@pau.edu
  • Dr. Yogesh Vikal: Principal Molecular Geneticist, School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-PB, Mailto: yvikal-soab@pau.edu

Timeline

Summer Intern

C&B Operations
06.2023 - 08.2023

Graduate Teaching Assistant

Montana State University Bozeman
01.2023 - Current

Graduate Research Assistant

Montana State University Bozeman
01.2022 - 12.2022

Research Project Trainee

Punjab Agricultural University
08.2020 - 08.2021

Summer Intern Researcher

Punjab Agricultural University
05.2019 - 07.2024

Bachelor of Science - Biotechnology

Punjab Agricultural University

Master of Science - Plant Pathology

Montana State University - Bozeman
HRITHIK PAHUJA