Ian Harris
Londonderry,New Hampshire
Overview
3
3
years of professional experience
Work History
Research Associate
Invivyd
11.2024 - Current
- Produced antibodies using yeast-based platform, testing for neutralization efficiency to support antibody discovery efforts.
- Taking these antibodies, I conducted pseudovirus neutralization assays tested on mammalian cells.
- Conducted pseudovirus neutralization assays on mammalian cells to evaluate antibody effectiveness.
- Utilize automated liquid handlers for maintenance, production, and purification of these antibodies.
- Additional tasks included data analysis, mammalian & yeast culture maintenance, ELISAs, and PCRs
Research Assistant II
Beth Israel Deaconess Medical Center
04.2024 - 10.2024
- Cloning, transformations, and Mini/Midipreps are performed to produce engineered plasmids, sequence check via Tn5.
- Utilizing a variety of plasmids, FreeStyle suspension cultures were transfected to produce protein, then purified to be used with lipid nanoparticles for analysis in the delivery of these particles to different locations in vivo.
- Adherent mammalian cells (HEKs) are transfected, then gene editing rates for Cas711 are screened using next gen sequencing.
- Sorted and categorized research materials to ensure easy access and efficient workflow, maintaining a well-ordered workspace.
Co-op - Organ Manufacturing
United Therapeutics
05.2023 - 10.2023
- Supported lung tissue engineering experiments using pulmonary cell types and 3D printed hydrogel models to advance research efforts.
- Examined lung epithelial population heterogeneity to establish a functional balance of basal, ciliated, and secretory cells.
- Targeted modulation of ciliated cell percentage using biomechanical cues such as NOTCH inhibition or air flow in perfusion models.
- Executed non-destructive and endpoint analysis techniques to quantify metabolites and secreted factors, imaging results with high-content and high-magnification approaches, and analyzing epithelial subpopulations via flow cytometry.
Education
Master of Science - Biotechnology - Industrial and Biomedical Sciences
University of New Hampshire
Manchester, NH05-2024
Bachelor of Science - Biotechnology
University of New Hampshire
Manchester, NH05-2022
Skills
- Pseudovirus Neutralization Assay
- Antibody production
- Protein purification
- ELISA
- PCR
- Cloning
- Transfection
- Bacterial Transformation
- Flow Cytometry
- Hamilton Liquid Handler
- Mammalian Cell Culture
- Immunocytochemistry
- Microsoft Office
Timeline
Research Associate
Invivyd
11.2024 - Current
Research Assistant II
Beth Israel Deaconess Medical Center
04.2024 - 10.2024
Co-op - Organ Manufacturing
United Therapeutics
05.2023 - 10.2023
Master of Science - Biotechnology - Industrial and Biomedical Sciences
University of New Hampshire
Bachelor of Science - Biotechnology
University of New Hampshire