Overview
Work History
Timeline
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Isidra Baker

Washington,IL

Overview

2
2
years of professional experience

Work History

Student

UICOMP Master's in Medical Biotechnology
Peoria, IL
05.2023 - Current
  • Conducted extensive research involving mouse and rat models to investigate the effects of MMP-12 suppression in transient focal cerebral ischemia.
  • Demonstrated proficiency in handling and husbandry of rodents, ensuring their well-being and compliance with ethical guidelines.
  • Designed and executed neurobehavioral evaluations to assess cognitive, somatosensory, balance, and locomotor function in rodent models.
  • Provided meticulous post-surgical care to animals undergoing experimental procedures, including monitoring for signs of distress and administering analgesics, antibiotics and fluids.
  • Implemented euthanasia procedures in accordance with ethical guidelines and approved protocols.
  • Administered subcutaneous and intraperitoneal injections for drug delivery, experimental manipulation and animal care.
  • Performed formalin and PBS perfusions to fix tissues for histological analyses.
  • Conducted tissue extraction procedures with precision and care to preserve tissue integrity including blood, spleen, brain and spinal cord.
  • Proficiently utilized histology techniques, including tissue fixation, imaging and embedding while assisting with cryosectioning, staining, and data analysis.
  • Executed molecular biology techniques such as qPCR, PCR, RNA extraction, Western Blotting, lysate preparation, and plasmid synthesis.
  • Contributed to the formulation of novel plasmid treatments.
  • Assisted in the creation of protocols to aid in the accurate execution of various experiments
  • Developed a comprehensive thesis proposal outlining a research project in neurobiology titled Assessment of the Role of MMP-12 in Post-ischemic Inflammation and the Impact of MMP-12 Suppression on Functional Recovery in Older Mice and Hypertensive Rats .
  • Conducted extensive literature review to establish theoretical framework and identify gaps in current knowledge.
  • Formulated clear research questions, hypotheses, and objectives to address the identified gaps.
  • Designed experimental methodologies, including data collection procedures and analytical approaches.
  • Created a detailed timeline and budget for the proposed research project.
  • Presented the thesis proposal to academic advisors and received constructive feedback for refinement.
  • Conducted a thorough review of existing literature related to the proposed research topic.
  • Evaluated the strengths and limitations of previous studies and methodologies.
  • Synthesized findings to provide context for the proposed research project.
  • Identified key theoretical concepts and methodological approaches relevant to the research objectives.
  • Critically analyzed the potential contributions of the proposed research to the field.
  • Co-mentored medical students and undergraduate students in lab techniques including and not limited to formulation, animal handling and various neurobehavioral assessments, RNA extraction, protein extraction, embedding, tissue extractions etc.

Student

UIC Rockford Master's in Medical Biotechnology
Rockford, IL
08.2022 - 05.2023
  • Utilized flow cytometry technology to analyze cellular populations based on their physical characteristics.
  • Designed and constructed plasmids using different vectors for various applications.
  • Performed molecular cloning techniques such as PCR, restriction digestion and ligation.
  • Monitored fermentation process parameters such as pH, temperature, and dissolved oxygen levels to optimize product yield.
  • Created databases to store experimental data collected during research activities.
  • Conducted cell culture maintenance and analysis with mammalian cell lines.
  • Cultured bacteria under controlled conditions to produce desired products.
  • Developed standard operating procedures for laboratory processes related to biotechnology projects.
  • Analyzed data from experiments to identify trends, patterns, or relationships among variables.
  • Analyzed protein expression profiles using Western blotting and ELISA technologies.
  • Maintained detailed records of experiments, results, and observations in laboratory notebooks.
  • Identified potential sources of contamination in the lab and developed appropriate solutions to reduce risk.
  • Constructed transgenic organisms by introducing foreign DNA into host genomes.
  • Prepared research reports summarizing findings from biotechnological experiments.
  • Implemented analytical tools like bioinformatics software packages for sequence analysis and comparison.
  • Maintained accurate records of experimental procedures and results.
  • Prepared and presented scientific findings to stakeholders.
  • Ensured compliance with ethical guidelines in research activities.
  • Used bioinformatics applications in proteomics and metabolomics.
  • Communicated research results through conference presentations, scientific publications or project reports.
  • Performed statistical analyses of molecular biology data sets.
  • Conducted literature reviews to inform experimental design.
  • Collaborated with interdisciplinary teams on research projects.
  • Analyzed protein structures using molecular modeling techniques.
  • Improved professional knowledge by reading bioinformatics literature.
  • Programmed scripts for automating tasks in Python and R, languages.
  • Participated in proposal writing and product development projects.
  • Learned and mastered animal handling techniques for laboratory rodents.
  • Acquired proficiency in various injection techniques, including intraperitoneal, subcutaneous, intravenous, submandibular, retro-orbital, and cardiac puncture injections.
  • Developed skills in tail snipping for blood collection methods from rodents.
  • Received training in aseptic technique and surgical procedures, including simple continuous and simple interrupted suturing.
  • Assisted in the development and submission of research protocols to the Institutional Review Board (IRB), ensuring compliance with regulatory standards and ethical guidelines.

Timeline

Student

UICOMP Master's in Medical Biotechnology
05.2023 - Current

Student

UIC Rockford Master's in Medical Biotechnology
08.2022 - 05.2023

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Isidra Baker