Highly skilled Lab Technician with background in conducting scientific tests and analyses. Demonstrated capability in wet lab techniques and rodent models, maintaining safety protocols, and data analysis and presentation. Strengths include problem-solving abilities, analytical skills, and strong familiarity with quality control procedures. Contributed to significant advancements in research projects leading to multiple publications by providing data collection, data interpretation, and scientific writing.
- Assisting in researching dietary effects on cancer development and mortality.
- Mouse Care Experience:
· Husbandry including breeding and weaning.
· Colony Management; weighing, tumor measurements, monitoring health, genotyping by tail clip.
· Blood collection via tail bleed, cheek bleed, cardiac bleed
· SC, IP, IM injections, gavages
- Mouse Surgery Experience:
· Lead surgeon and tasked with training others.
· Performing orthotopic prostate injection.
· Performing survival surgeries.
· Performing general necropsies, organ removal, and tumor collection.
· Primary cell extraction and extension.
- Wet Bench Techniques
· Histology tasks include embedding, slicing, tissue staining, and labeling.
· Protein extraction and evaluation by Western blot.
· RNA isolation for PCR, qPCR, and rtPCR.
· Basic cell culture techniques include culture extension, freezing/storing cells and cell assays
· Preparation of media and reagents
· ELISA’s
- Laboratory Management
· Tracking laboratory inventory and supplies ordering.
· Maintaining hazardous chemicals databases and proper storage.
· Lab safety manager.
- Misc
· Cleaning, sterilization of glassware using dishwasher and autoclave
· Sterilization of liquid solutions, surgery tools, mouse food, and glassware
- Researching the efficacy of experimental medications from NIDA on preventing addiction relapse in a rat model of self-administration.
- Rat Care Experience:
o Husbandry including breeding and weaning.
o Colony Management; weighing and monitoring health.
- Rat Surgery/Experiment Experience:
o Jugular catheterization surgery.
o Medicinal injections.
o Self-administration behavioral testing.
- Data input, analysis, and figure generation.
- Maintaining lab supply stock.
Dangerous Goods Shipping Certification 2024
Murine Technique Certifications 2022-2023
Virginia Commonwealth University
- Mouse 1.0: Restraint, IP, and SQ Injections
- Inhalant Anesthesia and Monitoring
- Rodent Blood Collection
REVIVE! Naloxone Trainer.
SAARA of Virginia 2017
- Certification to administer naloxone and to train others to administer naloxone.
Peer Recovery Coach
Virginia Commonwealth University Monroe Park Campus 2018
Krisanits, B. A., Schuster, R., Randise, J., Nogueira, L. M., Lane, J. T., Panguluri, G. A., Li, H., Helke, K., Cuitiño, M. C., Koivisto, C., Spruill, L., Ostrowski, M. C., Anderson, S. M., Turner, D. P., & Findlay, V. J. (2023). Pubertal exposure to dietary advanced glycation end products disrupts ductal morphogenesis and induces atypical hyperplasia in the mammary gland. Breast cancer research : BCR, 25(1), 118. https://doi.org/10.1186/s13058-023-01714-4
Manicka, S., Zhang, F., Celik, E., Lane, J., Pottanat, E., & Fossen, M. V. (2022). The Use of Repeated Forced Social Defeat as an Animal Stress Model. IMPULSE, 19(1). https://impulse.pubpub.org/pub/pvyzzhbd
Zhang, F., Hillhouse, T. M., Anderson, P. M., Koppenhaver, P. O., Kegen, T. N., Manicka, S. G., Lane, J. T., Pottanat, E., Van Fossen, M., Rice, R., & Porter, J. H. (2021). Opioid receptor system contributes to the acute and sustained antidepressant-like effects, but not the hyperactivity motor effects of ketamine in mice. Pharmacology, biochemistry, and behavior, 208, 173228. https://doi.org/10.1016/j.pbb.2021.173228