Jacob Coyne
Rougemont,NC
Summary
Dedicated and highly skilled research scientist with over 6 years of experience in assay development in the biotech industry. A proven track record of delivering successful results through innovation and creative problem solving in a high-velocity R&D setting. Proficient in a wide range of laboratory techniques and data analysis, with a focus on delivering robust and reliable assays for industrial applications.
Overview
8
8
years of professional experience
Work History
Assay Development Scientist
AgBiome
11.2022 - Current
- Led the development, validation, and implementation of high-throughput screening assays for characterizing a microbial library (>120K isolates) with phenotypic data.
- Collaborated with an interdisciplinary data science team to strategize and develop solutions for targeted screening of diverse microbes.
Assay Specialist
Altis Biosystems, Inc.
04.2022 - 11.2022
- Managed primary stem cell culture molecular and biochemical assays including IC50, cytotoxicity, ICC and fluorescence microscopy, and gene and protein expression analyses (ELISA, Luminex, BioMark).
- Contributed to R&D projects developing novel assays to characterize proprietary primary stem cell culture platforms.
Scientist III
Baebies, Inc.
01.2021 - 03.2022
- Identified and classified design requirements and limitations during project feasibility phases.
- Assessed, optimized, and validated assay performance such as sensitivity, linearity, dynamic range, stability, interference, and method agreement following CLSI-FDA guidelines.
- Collaborated with software and hardware engineers to develop and assess new technologies.
- Supported the development of a SARS-nCoV-2 RT-PCR molecular assay and platform currently on the market.
- Delivered presentations on current projects at company and team meetings.
Scientist II
Baebies, Inc.
01.2019 - 12.2020
- Successfully delivered a heparin monitoring project from R&D feasibility to product development.
Scientist I
Baebies, Inc.
01.2018 - 12.2018
- Contributed to multiple NIH grants to develop, optimize, and multiplex a variety of analytical assays.
- Developed a chemiluminescence detection method with improved sensitivity over a fluorescence-based assay.
- Demonstrated feasibility for detection of fentanyl in meconium and urine using a homogenous immunoassay on an automated microfluidic device; resulted in NIH grant award through the HEAL Initiative.
- Established and evaluated a digital microfluidic GALT assay for a $183k NIH SBIR grant.
- Produced protein C, ATIII, and factor VIII assays for a $215k Phase I NIH SBIR grant; $763k Phase II awarded.
Assay Development Intern
Baebies, Inc.
06.2017 - 12.2017
- Constructed a clinical diagnostic hypoglycemia assay panel comprised of six biomarker tests utilizing functional enzymatic and immunoassays for Phase I of a fast-track NIH SBIR grant; $682k Phase II awarded.
- Optimized assay designs and reagent formulations.
- Designed and carried out focused studies to characterize assay performance across various analytical metrics.
- Analyzed experimental results and provided protocols and oral presentations.
- Collaborated with grant writers to author reports by providing technical information including graphs and statistics.
Research Assistant
Neurobiology lab, Molecular Biomedical Sciences, CVM, NCSU
04.2016 - 08.2016
- Utilized techniques including PCR, ISH, IHC, DNA/RNA extraction and isolation, cell culture, and calcium imaging to identify, characterize, and elucidate pruritogenic & nociceptive pathways in genetically modified knockout mice.
Education
B.S. in Biological Sciences: Integrative Physiology and Neurobiology -
North Carolina State University
Raleigh, NC12.2017
Skills
- Assay development
- Acid/base chemistry
- Digital microfluidics
- RT-PCR
- Gel electrophoresis (SDS-PAGE)
- Design of Experiments (DOE)
- Enzymatic assays
- UV/VIS Spectrophotometry
- Jupyter notebook (Python)
- Immunoassays (ELISA, EMIT)
- ICC/IHC/ISH
- Statistical analysis (Prism, Excel)
- DNA/RNA extraction/isolation/quantification
- Fluorescence microscopy
- Technical writing
- Bioanalyzer, TapeStation, Nanodrop, Qubit
- Image analysis (CellProfiler)
- Cell culture
Coursework
- General Chemistry I & II
- Inorganic Chemistry
- Advanced Human Anatomy & Physiology
- Organic Chemistry I & II
- Cell Biology
- Biotechnology courses: Principles of Biology I & II
- Endocrinology
- Manipulation of Recombinant DNA
- Biochemistry I
- Neurobiology
- Protein Interactions
- Physics I & II
- Genetics
- Mapping the Brain
- Calculus I & II
- Statistics
Presentations
- Kennedy A, Coyne J, Singh R, Sista R, Norton S, Vamsee P. A Novel Newborn Screening Device for Illicit Drug Exposure. American Association for Clinical Chemistry Annual Scientific Meeting, Aug 2019. (poster)
- Emani S, Coyne J, Nelson L, Kennedy A, Singh R, Norton S, Rista R, Pamula VK, Emani SM. Digital Microfluidic Platform for Heparin Monitoring in Pediatric Patients using Small Volume Blood Samples. Pediatric Academic Societies Annual Meeting, May 2019 & American Association for Clinical Chemistry Annual Scientific Meeting, Aug 2019. (poster)
- Cotten CM, Coyne J, Nelson L, Singh R, Norton S, Pamula V, Freemark M. Neonatal hypoglycemia: rapid assessment using a digital microfluidic platform. Pediatric Academic Societies Annual Meeting, May 2018. (poster)
- Wheeler J, Domenichiello A, Coyne J, DeBrecht J, Davis J, Ramsden C, Mishra S. K. Identification of a Novel Itch Inducing Compound. Annual Research Forum, College of Veterinary Medicine, NCSU, Aug 2016. (poster)
Publications
Sista R. S., Ng R., Nuffer M., Basmajian M., Coyne J., Elderbroom J., Hull D., Kay K., Krishnamurthy M., Roberts C., Wu D., Kennedy A., Singh R., Srinivasan V., Pamula V. K., Digital Microfluidic Platform to Maximize Diagnostic Tests with Low Sample Volumes from Newborns and Pediatric Patients. Diagnostics. 10, 21 (2020). Mishra S.K., Wheeler, J. J., Pitake, S., Ding H., Jiang C., Fukuyama T., Paps J. S., Ralph P., Coyne J., Parkington M., DeBrecht J., Ehrhardt-Humbert L. C., Cruse G. P., Baumer W., Ji R., Ko M., Olivry T., Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch. Cell Reports. 31, 107472 (2020).
Timeline
Assay Development Scientist
AgBiome
11.2022 - Current
Assay Specialist
Altis Biosystems, Inc.
04.2022 - 11.2022
Scientist III
Baebies, Inc.
01.2021 - 03.2022
Scientist II
Baebies, Inc.
01.2019 - 12.2020
Scientist I
Baebies, Inc.
01.2018 - 12.2018
Assay Development Intern
Baebies, Inc.
06.2017 - 12.2017
Research Assistant
Neurobiology lab, Molecular Biomedical Sciences, CVM, NCSU
04.2016 - 08.2016
B.S. in Biological Sciences: Integrative Physiology and Neurobiology -
North Carolina State University