Jacob Landeck
Milford,MA
Summary
I am a research scientist with six years of academic research experience and two years of industry research and development experience. I am interested in protein structure and function and have taken a research project from protein expression and purification through structure and model determination. While working in industry, I developed a medical device and worked through the product development and FDA regulation processes.
Overview
9
9
years of professional experience
Work History
Graduate Research Scientist
Kelch Lab
07.2019 - Current
- Lead several research projects to understand the structural and biochemical mechanisms of bacterial and viral proteins
- Collaborated with scientists across multiple research groups to establish assays, optimize protein purifications methods, and interpret results
- Assess how a protein complex's structure affected its function using structural techniques, mutagenesis, and protein kinetics/binding experiments
- Trained multiple laboratory members on various biochemical, structure determination, and protein model building techniques
Research Associate
Anika Therapeutics
05.2016 - 05.2018
- Responsible for the formulation, characterization, and FDA testing required for 510(k) clearance of an injectable calcium phosphate cement
- Worked with the Research and Development, Engineering, Manufacturing, and Quality Control Departments to accomplish goals related to the injectable calcium phosphate cement
- Worked on developing the IP for two patent applications.
Research and Development Intern
Anika Therapeutics
05.2015 - 08.2015
- Developed and performed assays to assess osteocyte differentiation in various cell types
- Maintained both mammalian and primary cell lines
- Interpreted, visualized, and reported research results to managers in the company
Education
Ph.D. - Biomedical Sciences
University of Massachusetts Chan Medical School
Worcester, MA04.2024
BS Biochemistry and Molecular Biology -
University of Massachusetts-Amherst
Amherst, MA05.2016
Skills
- Cryo-EM: cryoSPARC, Relion, cisTEM
- Model Refinement: Phenix
- Manual and Automated Model Building: Coot, StarMap, Isolde, Alphafold
- Cryo-EM sample preparation and screening: Vitrobot operation, grid preparation, sample quality assessment
- Protein Structure Visualization: Pymol, ChimeraX
- Protein Binding/Activity Assays: Absorbance based enzyme kinetics, anisotropy-based binding, Electrophoretic Mobility Shift Assay (EMSA), Western blotting
- Protein Expression: E coli protein expression and induction, strain optimization, vector optimization
- Protein Purification: Resin based purifications, Bio-Rad FPLC operation, affinity columns, cation/anion exchange, size exclusion chromatography
- Cloning: Gibson Assembly, Restriction Digestion, ligation, Quick change, Agarose gel
- Unix: command line, system operation
- Experimental design, data interpretation
Timeline
Graduate Research Scientist
Kelch Lab
07.2019 - Current
Research Associate
Anika Therapeutics
05.2016 - 05.2018
Research and Development Intern
Anika Therapeutics
05.2015 - 08.2015
Ph.D. - Biomedical Sciences
University of Massachusetts Chan Medical School
BS Biochemistry and Molecular Biology -
University of Massachusetts-Amherst
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