Jacob Loughry
Las Vegas,NV
Summary
Experienced Research Specialist with 5+ years of expertise in managing lab operations. Skills in quality control, aseptic technique, and microbiological method validation. Track record in overseeing equipment maintenance, inventory management, and providing lab training. Extensive experience in GMP, GLP regulations, and ISO standards.
Overview
6
6
years of professional experience
Work History
Staff Research Associate II
University of Nevada, Reno
04.2022 - Current
- Gathered and organized information for research purposes
- Clone genes using PCR, express proteins in E. Coli bacteria, and perform column chromatography to purify
- Grow and amplify antigens using BSL-2 bacteria, extract antigens through hot phenol technique while maintaining sensitive environmental controls
- Schedule animal studies, handle mice and administer vaccine formulations; perform tissue harvests, gather data; graph data and present results and recommendations to PI
- Culture mouse cell lines at BSL-1 and BSL-2; process blood and tissues from different animal species; perform immunoassays including LAL, ELISA, ELISpot and opsonphagocytosis assays
- Develop and schedule preventative maintenance for various lab equipment and liaise with equipment manufacturer's technical support
- Analyzed results using analytical software and created reports.
- Train and review the work of technicians and students
Biologist/Life Support Operator
Golden Nugget
07.2020 - 04.2022
- Provided daily care of animal exhibits including diet preparation, feeding, and general care for over 1000 animals
- Handled specimens and conducted tests according to established protocols, keeping efficient records on all experiments.
- Assisted with the diagnosis and treatment of various fish diseases
- Performed water quality testing of all exhibits using pH meters, DO meter, hydrometer, spectrometer, refractometer, microscope, ORP (oxidation/reduction potential) meter, electronic balances
- Performed Life Support System maintenance such as the plumbing and electrical work for sand filters, UV sterilizers, protein skimmers, pumps, and ozone generators
Research Technician
Arizona Game and Fish
02.2019 - 07.2019
- Valuable skills used such as GPS systems, biological data collection, radio procedure, data entry and analysis, organization, and various lab techniques to conduct proper research
- Supported senior management with creation and enhancement of continuous improvement of research procedures.
- Operated laboratory and experimental equipment such as PCR (and qPCR), microscopes, spectrophotometers, autoclaves, centrifuges, and analytical balances
- Worked with and safely disposed various toxic chemicals, reproductive hazards and carcinogens
Education
Bachelor of Science - Biology with Chemistry minor
Northern Arizona University
Flagstaff, AZ12-2018
Skills
- Microbiology
- Flow cytometry
- DNA Isolation
- Equipment Maintenance
- Scientific Writing
- Interdisciplinary Teamwork
- Inventory Management
- Laboratory Procedures
- Cell culture
- Immunology
Timeline
Staff Research Associate II
University of Nevada, Reno
04.2022 - Current
Biologist/Life Support Operator
Golden Nugget
07.2020 - 04.2022
Research Technician
Arizona Game and Fish
02.2019 - 07.2019
Bachelor of Science - Biology with Chemistry minor
Northern Arizona University
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