Jacob Thornsberry
Louisville,KY
Summary
Detail-oriented research assistant with extensive experience in animal model management, molecular biology, and various research. Adept at supporting complex in vivo studies, maintaining accurate data records, and collaborating on high-impact research. Passionate about translational biology and eager to contribute to innovative projects.
Overview
8
8
years of professional experience
Work History
Research Assistant II
University of Louisville Department of Immunology
Louisville, KY
04.2025 - Current
- Conduct cell culture experiments, including lentiviral transduction and infection assays, in support of translational research studies.
- Perform rodent surgeries and manage colony breeding, genotyping, and routine health monitoring.
- Execute multiparameter flow cytometry experiments, including sample preparation, staining, and data analysis.
- Support radiochemistry protocols and conduct in vivo PET/CT imaging for small animal models to assess biodistribution and therapeutic efficacy.
- Maintain detailed laboratory records and contribute to experimental design, data interpretation, and troubleshooting.
Laboratory Technician
Markey Cancer Center
Lexington, KY
05.2024 - 03.2025
- Conducted cell culture maintenance, transfection, and selection of stable cell lines.
- Performed molecular cloning, nucleic acid isolation (DNA/RNA), PCR, ELISA, and IHC staining.
- Maintained mouse colonies including breeding, weaning, genotyping (tail biopsy), and surgical preparation; assisted with injections and tissue collection for downstream analysis..
- Monitored and replenished lab supplies to maintain workflow efficiency.
- Recorded, organized, and analyzed experimental data using Microsoft Excel and Word.
- Prepared reports and presentations summarizing experimental results.
Research Assistant
Georgetown University Department of Immunology
Washington, D.C.
08.2023 - 05.2024
- Conducted research on reducing dengue virus transmission from mosquito vectors to human hosts, focusing on molecular and cellular aspects of host-pathogen interaction.
- Performed viral infection assays, RNA extraction, and qPCR to assess viral replication and transmission potential.
- Investigated immune modulation strategies in vector and host models to interrupt viral lifecycle stages.
- Maintained accurate records of experimental procedures and results for use in manuscripts and grant applications.
- Participated in lab meetings and collaborative discussions to refine methodologies, and troubleshoot technical challenges.
Research Assistant
Sanders-Brown Center on Aging
Lexington, KY
08.2022 - 07.2023
- Recruited and retained participants for clinical trials on experimental therapeutics.
- Conducted participant interviews and maintained HIPAA-compliant records.
- Created participant-facing materials and translated clinical protocols for public understanding.
- Supported coordination of outreach and engagement events.
Research Assistant
University of Kentucky Department of Biology
Lexington, KY
12.2020 - 05.2022
- Conducted research examining the relationship between gut microbiota and circadian rhythm dysfunction (chronobiology).
- Assisted in designing and implementing protocols to investigate microbiome influences on host internal clock regulation.
- Performed microbial culture, DNA extraction, and 16S rRNA sequencing sample preparation.
- Collected and analyzed physiological and behavioral data from animal models to assess circadian disruption.
- Executed statistical analysis and generated visual data summaries using Excel and graphing software.
- Contributed to lab meetings and collaborative manuscript preparation on microbiome-circadian interactions.
Server
First Watch
Lexington, KY
09.2018 - 12.2019
- Provided high-quality customer service in a fast-paced breakfast and lunch environment, managing multiple tables, and maintaining guest satisfaction.
- Accurately took and delivered food and beverage orders while coordinating with kitchen staff to ensure timely service.
- Handled customer concerns professionally, resolving issues promptly to ensure a positive dining experience.
- Maintained cleanliness and organization of the dining area, and adhered to health and safety standards.
- Processed payments efficiently, and balanced cash and credit transactions at the end of each shift.
- Worked collaboratively with team members to ensure smooth restaurant operations during peak hours.
Education
Graduate Certificate - Biostatistics
University of Louisville
Louisville, KY05-2026
Master of Science - Microbiology And Immunology
Georgetown University
Washington, D.C05-2024
Bachelor of Science - Biology
University of Kentucky
Lexington, KY12-2022
High School Diploma -
Lexington Christian Academy
Lexington, KY01-2018
Publications
- Mickelson, A. R., Felton, J., Cheschi, O., Spacone, E., Connors, K., Thornsberry, J., & Teramoto, T. (2025). Infections of Aedes Mosquito Cells by Wolbachia Strains wAu and wMelpop Modulate Host Cellular Transcriptomes Differently and Suppress Dengue Viral Replication. Viruses, 17(7), 922. https://doi.org/10.3390/v17070922
Timeline
Research Assistant II
University of Louisville Department of Immunology
04.2025 - Current
Laboratory Technician
Markey Cancer Center
05.2024 - 03.2025
Research Assistant
Georgetown University Department of Immunology
08.2023 - 05.2024
Research Assistant
Sanders-Brown Center on Aging
08.2022 - 07.2023
Research Assistant
University of Kentucky Department of Biology
12.2020 - 05.2022
Server
First Watch
09.2018 - 12.2019
Master of Science - Microbiology And Immunology
Georgetown University
Bachelor of Science - Biology
University of Kentucky
High School Diploma -
Lexington Christian Academy
Graduate Certificate - Biostatistics
University of Louisville