Jasleen Singh
Oakland,CA
Summary
Accomplished researcher with 15 years of experience in molecular cloning and heterologous protein purification systems. Proven track record in assay development and NGS, leading to impactful findings in the fields of epigenetics, transcription, RNA trafficking, and exosome biology. Proficient in diverse cellular and biochemical techniques, fostering innovative research initiatives.
Overview
13
13
years of professional experience
Work History
Postdoctoral Fellow
HHMI, University of California Berkeley
Berkeley, California
10.2020 - Current
- Conducted comprehensive investigations into the molecular and biochemical mechanisms of exosome biogenesis, and RNA trafficking across organelles and cells.
- Developed experimental procedures for rapid immuno-isolation of subcellular organelles, facilitating ultra-low input RNA sequencing through multiple advanced Next-Gen Sequencing (NGS) platforms.
- Executed in vitro biochemical reconstitution of complex RNA trafficking, utilizing purified proteins, cellular membranes, and nucleic acids.
- Collaborated with interdisciplinary teams to enhance research project outcomes.
- Authored scientific publications detailing experimental results and analyses, and mentored students conducting independent research projects.
Postdoctoral Fellow
Indiana University
Bloomington, Indiana
06.2019 - 09.2020
- Designed assays to investigate chromatin association mechanisms for various proteins involved in transcriptional gene silencing (TGS), leveraging cutting-edge techniques like CUT and RUN, and CUT and TAG.
- Acquired training in bioinformatics tools, enabling a thorough examination of substantial genomic datasets resulting from the experiments above.
- Conducted negative stain and cryo-electron microscopy to investigate molecular action mechanisms of purified recombinant proteins and protein complexes associated with TGS
- Published scientific papers outlining experimental findings and analyses, while guiding students in independent research efforts.
Graduate Research Assistant
Indiana University
Bloomington, IN
08.2012 - 05.2019
- Research and development in the field of epigenetics, transcription, RNA interference, nucleic acid, and protein biochemistry.
- Developed in vitro reconstitution assays for DNA-dependent RNA polymerases (DdRPs), RNA-dependent RNA polymerases (RdRPs), Dicer enzymes, argonaute proteins, helicases, and nucleosome remodeling factors, using native and recombinant protein complexes.
- Discovered biochemical mechanisms driving the biogenesis of small RNAs essential for genome defense and stability through the silencing of transposons and repeat elements via DNA methylation and chromatin modifications.
- Developed deep expertise in molecular cloning, expression, and purification of recombinant proteins, utilizing various prokaryotic and eukaryotic systems.
Accomplished cloning and expression of four 12-subunit enzyme complexes, utilizing baculovirus-mediated insect cell expression systems. - Published scientific papers outlining experimental findings and analyses, while guiding students in independent research efforts.
- Collaborated with faculty and peers on interdisciplinary research initiatives.
Education
Ph.D. - Biology And Biochemistry
Indiana University, Bloomington
Bloomington, IN05-2019
Master of Science - Plant Pathology
The Ohio State University
Columbus, OH05-2012
Bachelor of Science - Genetics
Punjab Agricultural University
Ludhiana, Punjab, India07-2009
Skills
- Molecular cloning
- RNA sequencing and NGS
- Protein purification
- Assay development
- Exosome biology
- Genome research
- Cell culture techniques
- Cryo-electron microscopy
- Bioinformatics analysis
- Mentorship and writing
- Interdisciplinary collaboration
Affiliations
Racket sports | Fermentation | Hiking
References
References available upon request.
Timeline
Postdoctoral Fellow
HHMI, University of California Berkeley
10.2020 - Current
Postdoctoral Fellow
Indiana University
06.2019 - 09.2020
Graduate Research Assistant
Indiana University
08.2012 - 05.2019
Ph.D. - Biology And Biochemistry
Indiana University, Bloomington
Master of Science - Plant Pathology
The Ohio State University
Bachelor of Science - Genetics
Punjab Agricultural University
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