JEFFREY D. CARTER

Experienced director of chemistry process research, scale-up, development, and production with expertise in project planning and management. Implemented supply chain management processes for just-in-time inventory, resulting in annual savings of over 500k. Skilled in DNA/RNA solid phase synthesis, high and low pressure chromatography, conjugation, ultrafiltration, and lyophilization. Proficient in HPLC, UPLC, LC/MS, TLC, and other analytical methods. Implemented quality systems and tracking from raw material control through finished goods for approved therapeutic and materials for Laboratory Developed Tests. Familiar with 21 CFR parts 210 and 820. Strong problem-solving skills. Proficient in MS Office suite, MS Project, and Visio.

• Gawande, Bharat N. et al, “Selection of DNA aptamers with two modified bases,” PNAS Early Edition 2017
• Carlson, Michelle, Carter, Jeffrey D, & Rohloff, John, “Improved preparation of 2 M triethylammonium bicarbonate,” Green Chemistry Letters and Reviews (2015), 8:3-4, 37-39
• Rohloff, John C. et al, “Practical Synthesis of Cytidine-5-Carboxamide-Modified Nucleotide Reagents,” Nucleosides, Nucleotides, and Nucleic Acids 2015, 34:180-198
• Gelinas, Amy D. et al, “Crystal Structure of Interleukin-6 in Complex with a Modified Nucleic Acid Ligand,” Journal of Biological Chemistry 2014, 289(12)
• Davies, Douglas R. et al, “Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets,” PNAS Early Edition 2012
• Gupta, Shashi et al, “Rapid Histochemistry Using Slow Off-rate Modified Aptamers With Anionic Competition,” Appl Immunohistochem Mol Morphol 2011 Jan 7
• Gold, Larry et al, “Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery,” PLoS ONE 5(12), Dec 7, 2010
• Vaught, Jonathan D., et al, “Expanding the Chemistry of DNA for In Vitro Selection,” J. AM. CHEM. SOC. 2010, 132, 4141–4151
• Hill, Kenneth W. et al, “Diels-Alder Bioconjugation of Diene-Modified Oligonucleotides,” J. Organic Chem. 2001, 66, 5352-5358
• Bridonneau, Philippe et al, “Purification of a highly modified RNA-aptamer: Effect of complete denaturation during chromatography on product recovery and specific activity,” J. Chromatography B: Biomedical Sciences and Applications, Volume 726, Issues 1-2, 16 April 1999, Pages 237-247
• Vargeese, C et al, “Efficient Activation of phosphoramidites by 4,5-Dicyanoimidazole,” Nucleic Acids Research, Feb 1998
• Yu, Lin, Ternansky, Robert J, Carter, Jeff, “The Synthesis of Nucleoside Linker Molecules for Incorporation intoTherapeutic Oligonucleotides,” ACS poster, La Jolla Pharmaceutical, 1994

• US 9,163,056 B2 5-Position modified pyrimidines and their use
• Two additional filed patents, not issued

Equipment knowledge: Analytical and Preparative scale HPLC systems (Hewlett-Packard 1100, 1090 asnd 1050, Shimadzu 430A, Beckman System Gold, Rainin Dynamax, Waters Delta-Prep 4000, and BioCAD 60 and 250), Fmoc and tboc peptide synthesizers (MilliGen and ABI), rotary evaporators, UV spectrophotometers, peptide/protein sequencer (Milligen), lyophilizers, centrifuges, and DNA synthesizers (Milligen 8750, 8800 and Large Scale Biology PCOS, ABI 3900, MerMade 192, 192X, and 12), OligoPilot I/II.

US 9,163,056 B2 5-Position modified pyrimidines and their use, Gawande, Bharat N. et al, “Selection of DNA aptamers with two modified bases,” PNAS Early Edition 2017, Carlson, Michelle, Carter, Jeffrey D, & Rohloff, John, “Improved preparation of 2 M triethylammonium bicarbonate,” Green Chemistry Letters and Reviews (2015), 8:3-4, 37-39, Rohloff, John C. et al, “Practical Synthesis of Cytidine-5-Carboxamide-Modified Nucleotide Reagents,” Nucleosides, Nucleotides, and Nucleic Acids 2015, 34:180-198, Gelinas, Amy D. et al, “Crystal Structure of Interleukin-6 in Complex with a Modified Nucleic Acid Ligand,” Journal of Biological Chemistry 2014, 289(12), Davies, Douglas R. et al, “Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets,” PNAS Early Edition 2012, Gupta, Shashi et al, “Rapid Histochemistry Using Slow Off-rate Modified Aptamers With Anionic Competition,” Appl Immunohistochem Mol Morphol 2011 Jan 7, Gold, Larry et al, “Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery,” PLoS ONE 5(12), Dec 7, 2010, Vaught, Jonathan D., et al, “Expanding the Chemistry of DNA for In Vitro Selection,” J. AM. CHEM. SOC. 2010, 132, 4141–4151, Hill, Kenneth W. et al, “Diels-Alder Bioconjugation of Diene-Modified Oligonucleotides,” J. Organic Chem. 2001, 66, 5352-5358, Bridonneau, Philippe et al, “Purification of a highly modified RNA-aptamer: Effect of complete denaturation during chromatography on product recovery and specific activity,” J. Chromatography B: Biomedical Sciences and Applications, Volume 726, Issues 1-2, 16 April 1999, Pages 237-247, Vargeese, C et al, “Efficient Activation of phosphoramidites by 4,5-Dicyanoimidazole,” Nucleic Acids Research, Feb 1998, Yu, Lin, Ternansky, Robert J, Carter, Jeff, “The Synthesis of Nucleoside Linker Molecules for Incorporation intoTherapeutic Oligonucleotides,” ACS poster, La Jolla Pharmaceutical, 1994

• Hewlett - Packard HPLC Training: Methods Development, 11/1993
• Hewlett - Packard HPLC Training: Maintenance and Troubleshooting, 11/1993
• Waters Millenium training, Spring 1998
• Initial and follow-up emergency response team training (ERT) and Hazcom training
• Continuous training on operations of Agilent LC/MS systems and Waters LC systems