Jessica Gallagher

Maintained 8 air bnb properties. Report and help resolve issues of the rentals. Also, created landscaping and upkeeping of the properties.

• PCR Tolerance planting of soybean.
• Inoculations of soy seedling samples.
• Soy seed plant germinations.
• Gene ID planting and inoculations.
• Phytophthora Root Stem Rot resistant and susceptible.
• BSR planting and inoculations.
• Key Contributions:

• Grow plants to test different resistant and susceptible diseases. (Bacteria Blight, Verticillium, Xanthamonas, Corranespora, Barbren, Wheat BLS, Fusarium, Reniform)
• Grow cultures, make inoculum, inoculate, thin, randomize, fertilize, monitor, rate, and take pictures for diseased plants.
• Planted wheat for Culture Xanthamonas Translucent Inoculations.
• RKN extraction and culture pot inoculations.
• Plant and inoculate Vert soil samples.
• Surface extractions of microbes from fresh tissue.
• Fungal DNA extractions on BLS for Fusarium.
• Sugar Sucrose Reniform extraction for sieving nematodes from tomato plants.
• Collect isolates for fungal and bacteria cultures to store in LB bead bank.
• Run chlorophyll readings using meter.
• Run quantitative real time PCR on tissue samples and nanodrop concentration.
• Make and pour gels for photodigm.
• Make reagents (LB, glycerol, primers, 2x loading dye, TE buffer, supermix, twine, and ladders for gels)
• Inventory, store or toss all processed samples.
• Dehydrate samples and freeze in GLY to ship.
• Installed drip irrigation in grow chambers.
• Made protocols for all lab tasks and procedures.
• Received training on Cotton Ginning, Respirator training, Growth Chamber safety training, Field Safety training, Pesticide training.
• Key Contributions:

• Sent out daily detailed mails of tasks completed in the lab.
• Ran test runs on Bullet Extractions.
• Completed mandatory yearly electronic lab inspections.
• Made protocols for lab procedures to be published.
• Trained new employees.
• Ran Fluidigm chips for Native Traits.
• Made daily log checklists for machines that included cleaning, program errors, PM's, repairs, reboots, closed out ticket repair for specialized techs, and fixing and trouble shooting strategies. (biomeks, liquid handlers, centerfuges, cyclers, mega grinders, seed counters, hoods, spectro plate readers, nanodrops, and plate readers)
• Helped work in other labs. (helped corn on Metanomics Sample Prep)
• Ran Master Chem, Excel, LIMS, Windows 7, Microsoft Word programs.
• Key Contributions:

• Prep cotton samples for extractions. (count, grind, scoop, and plate)
• Processed production and BME Extractions. (ground seed, lyophilized leaf, fresh leaf tissue, single leaf, and bulk leaf)
• Ran Real Time PCR and ENDPoint PCR reactions on samples. (cycle, spectro read, and score samples)
• Make mixes, nanodrop, aliquot, run QC's, check in, receive, order, inventory to database and stock all reagents for the lab.
• Received Rapid Recognition for completing an urgent project above and beyond expectations for being flexible, reliable, and having tremendous task management skills!
• Key Contributions:

• Sequencing large and small scale PCR set ups, qualified for operation and monitoring of lab equipment with precision and care, responsible for special tasks requiring constant surveillance and safety involving biotech machines and freezers, loaded 377 and 3730 ABI machines, picked agar plates with Qbot and Qpic, operated M13 plaque picker, back preparation with phenol, RNA digest, sequenced and re-arrayed trays biomek Hamilton and EPI, normalized DNA, primer validation, clean up precipitations, ran gels with photodyne and DNA purification.
• Consistently commended for the timely, high-quality completion of both routine and special laboratory DNA samples of patient specimens.
• Ensured test-result validity before recording/reporting results, earning a reputation for meticulous attention to detail.
• Demonstrated the ability to communicate test results with staff as a member of a team focused on providing exemplary quality of care.
• Evaluated quality control within laboratory using standard laboratory test and measurement controls, and maintained compliance with environmental health and safety codes.
• Key Contributions:

• Responsibilities included transporting important documents to and from various courthouses in Illinois and Missouri, transcribing depositions and medical summaries.
• Created current closed filing system used by the Law firm
• Dictaphone transcription
• Safe and timely delivery of sensitive material
• Key Contributions: