JESUS VELAZQUEZ
Los Angeles
Summary
Adept at pioneering neurobiological research, honed my expertise in immunohistochemistry, cell analysis, and collaborative problem-solving. I have conducted a high volume of molecular biology techniques and employed a broad range of experimental methods, significantly contributing to understanding neuronal lipid metabolism and cortical excitation gradients. My work reflects strong analytical skills and an innovative approach to complex biological questions.
Overview
3
3
years of professional experience
Work History
Research Assistant( Geschwind Lab)
University of California, Los Angeles
09.2023 - Current
- Research involved assisting with inducing clonal hiPSC lines to NPCs and excitatory neurons via dox-inducible NGN2 overexpression
- Utilized immunohistochemistry techniques to study cell morphology, focusing on protein localization and expression patterns
- Performed detailed analysis of morphological features, including neurite area to evaluate differentiation outcomes across clonal lines
- Examined experiment results to identify potential underlying causes for scientific findings.
- Conducted comprehensive literature reviews to support ongoing research projects.
Summer Research Scholar
Broad Institute of Harvard/MIT
05.2023 - 08.2023
- Researched the role of astrocytes and neurons in lipid homeostasis, focusing on the inhibition of cholesterol influx through LRP1 receptor knockout using CRISPRi
- Established the role of lipid metabolism in neuronal deficits using a pluripotent stem cell-based model, contributing to a deeper understanding of neurobiological mechanisms
Research Associate RA1
University of California, Los Angeles
11.2021 - 02.2023
- Research focuses on the macroscopic gradient of excitation and inhibition across the cortex using functional and histological analyses
- Investigated calcium dynamics and the distribution of somatostatin, parvalbumin, and excitatory neurons in organotypic cultures from frontal and sensory cortical regions
- Findings show the frontal cortex has higher dimensional spontaneous activity and greater parvalbumin neuron density than the sensory cortex
- Studied comparative neural dynamics between the auditory and frontal cortex, identifying distinct functional differences and local network properties
- Utilized immunohistochemistry techniques to study and quantify protein expression across cortical regions
Education
B.S. - Biological Sciences
University of California, Los Angeles (UCLA)
06.2025
Skills
- Immunohistochemistry
- Histology
- Calcium Imaging
- Confocal Microscopy
- Primer Design
- PCR
- Western Blots
- DNA Extraction
- IPSC Tissue Culture
Awards
- Broad Institute of Harvard/MIT - Summer Research Scholar
- UCLA Undergraduate Fellows - Research Program Award
- NIH Diversity Grant (R01NS116589 - Multiplexing Working Memory and Timing)
- UCLA CAMP Fellowship Award, 06/01/23
- UCLA Life Science Dean's Award, 06/01/22
- UCLA Life Science Dean's Award, 06/01/23
- UCLA Life Science Dean's Award, 06/01/24
- Bruins in Genomics Scholar
- NIH Step Up Awardee
Languages
English
Native/ Bilingual
Spanish
Native/ Bilingual
Timeline
Research Assistant( Geschwind Lab)
University of California, Los Angeles
09.2023 - Current
Summer Research Scholar
Broad Institute of Harvard/MIT
05.2023 - 08.2023
Research Associate RA1
University of California, Los Angeles
11.2021 - 02.2023
B.S. - Biological Sciences
University of California, Los Angeles (UCLA)
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