Jia Bian
New York,USA
Summary
A strong background in molecular and cellular biology, protein biochemistry and cancer biology. Proficiency in genomics, epigenetics and proteomics. Considerable research experience in dissecting signal transduction pathways, gene expression regulation and molecular disease mechanisms. Experienced with training in the R & Python languages and with bioinformatics analysis of sequencing and genetic screening data (RNA-seq, ChIP-seq & CRISPR screening). Capable of designing and conducting research independently. Disciplined and motivated to working hard and being a contributing team member.
Overview
8
8
years of professional experience
Work History
Postdoctoral Researcher
Cold Spring Harbor Laboratory
02.2025 - Current
- Performed aseptic adherent cell culture including glioblastoma cell lines and primary mouse embryonic fibroblasts, thawing of cryovials, cell counting (automated and manual) and growth media preparation
- Performed sgRNA plasmid cloning; constructed glioblastoma cells with Dox-inducible sgRNAs for targeted gene deletions
- Performed overexpression and proliferation assays to assess impacts of targeted genes in carcinogenesis
- Performed drug screening to detect effects of drugs in inhibiting glioblastoma development
- Engaged in bioinformatics training sessions including analysis of ATAC-seq and scRNA-seq
- Supervised rotated PhD students
- Managed and drove an independent research project; performed experiments for grant application
PhD Candidate
Hudson Institute Of Medical Research
11.2018 - 01.2023
- Performed aseptic adherent cell culture including cryopreservation and liquid nitrogen handling, thawing of cryovials, cell counting (automated and manual) and growth media preparation
- Performed DNA and RNA isolation from human cancer cell lines; measured concentration/purity on Nanodrop
- Designed primers and performed RT-qPCR to detect and quantify mRNA levels for evaluating changes in gene expression
- Designed and cloned sgRNAs into lentiGuide-Puro plasmid for CRISPR-Cas9-based gene editing
- Performed bacterial transformation of plasmids, lentiviral production in HEK293T cells (including lentiviral supernatant concentration and viral titer measurement) and lentiviral transduction in host cancer cells
- Constructed stably C-BERST (dCas9-APEX2) - expressing cancer cell lines via lentiviral transduction followed by fluorescence-activated cell sorting and flow cytometry for C-BERST-positive cell selection and validation
- Assessed C-BERST localization efficiency via chromatin immunoprecipitation followed by qPCR; validated C-BERST-induced in vivo biotinylation specificity via western blotting
- Performed C-BERST-directed proximal biotinylation followed by tandem mass tag (TMT) quantitative mass spectrometry to define proteomic compositions at specific genomic sites
- Performed bioinformatics analysis of RNA-seq, ChIP-seq and CRISPR-Cas9 genome-wide loss-of-function screening data
- Published literature review and research data; developed confident verbal and communication skills for data presentation in meetings and at conferences
- Performed training and education for western blot imaging systems
Internship Student
Walter And Eliza Hall Institute Of Medical Research
12.2017 - 02.2018
- Learned and applied established codes to interrogate genomic databases and visualization packages
- Learned and applied computer coding language R to construct mock microbiomes and produce simulated nanopore long reads
- Learned and applied biological and mathematical concepts for in silico microbiome re-construction studies
- Developed critical thinking and problem-solving abilities
- Communicated with supervisors and staff efficiently and accepted suggestions for areas of improvement
Education
Ph.D. - Biomedical Science
Monash University
Australia01.2023
Master of Science - Biotechnology
The University of Melbourne
Australia07.2018
Bachelor of Science - Biotechnology
Shenyang Agricultural University
China07.2014
Skills
- Mammalian cell culture
- Flow cytometry and FACS
- Transfection and transduction
- CRISPR-Cas9 gene editing
- ChIP & Co-IP
- Proximity biotinylation (dCas9-APEX2)
- Bioinformatics analysis
Accomplishments
Collaborated with click biochemistry and structural biology specialists to design and develop new drugs for glioblastoma
Independently completed proximity biotinylation (dCas9-APEX2), the method had never been undertaken in the lab before; identified novel drug targets for colorectal cancer, the targets is undergoing investigation in vivo (PhD)
Supervised a team of students and staff members
Affiliations
- The Royal Society of Victoria - student member
- American Association for Cancer Research - student member
Timeline
Postdoctoral Researcher
Cold Spring Harbor Laboratory
02.2025 - Current
PhD Candidate
Hudson Institute Of Medical Research
11.2018 - 01.2023
Internship Student
Walter And Eliza Hall Institute Of Medical Research
12.2017 - 02.2018
Master of Science - Biotechnology
The University of Melbourne
Bachelor of Science - Biotechnology
Shenyang Agricultural University
Ph.D. - Biomedical Science
Monash University
PUBLICATIONS
- Bian, J. ; Dannappel, M.; Wan, C.; Firestein, R., Transcriptional Regulation of Wnt/β-Catenin Pathway in Colorectal Cancer. Cells 2020, 9 (9).
- Wan, C.; Mahara, S.; Sun, C.; Doan, A.; Chua, H. K.; Xu, D.; Bian, J. ; Li, Y.; Zhu, D.; Sooraj, D.; Cierpicki, T.; Grembecka, J.; Firestein, R., Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signaling identifies therapeutic targets for colorectal cancer. Sci Adv 2021, 7 (21).
AWARDS
Graduate Research Completion Award – Monash University