Jingruo Zhang
Palo Alto,CA
Summary
Dynamic and Inventive scientist with a Ph.D. In Physiology, backed by over 10 years in laboratory research. Expertise in molecular, cellular biology and biochemistry, extensive experience in biological assay development to uncover and validate novel therapeutic targets. Specialize in high throughput screening and RNA-Seq analysis. A strong understanding of biology through creativity in problem-solving and a commitment to turning scientific discoveries into viable treatments. Authorized to work in the United States without sponsorship.
Overview
11
11
years of professional experience
Skills
- Research and development
- Project Management
- Interdisciplinary Coordination
- Proficient in CRISPR/Cas9 screening
- Assay development experience
- Data analysis on Bulk and Single-cell RNA Sequencing
- Advanced Research Techniques
- Stem cell experience
- Proficient in Multi-color FACS
- Antibody/protein purification
Work History
Postdoctoral Research Scientist
University Of Texas Health Science At San Antonio
04.2019 - 01.2024
- Designed and led pioneering research on osteocyte Connexin 43 (Cx43), delving into its novel paracrine effects within bone marrow niche.
- Performed multi-color FACS followed by single-cell RNA sequencing in bone marrow niche, uncovering complicated crosstalk between hematopoietic cells and mesenchymal stromal cells.
- Employed HPLC-MS and ELISA to identify metabolites released from osteocyte Cx43 Hemichannels, collecting and interpreting data, confirming function of the released prostaglandins as the most pivotal factors.
- Introduced a proton fluorescence reporter into cells to show that mitochondrial Cx43 Hemichannels shield cells from oxidative stress by serving as H+ channels, reducing cell mortality. This provides key insights into cell defense mechanisms and positions Cx43 as a therapeutic target.
- Collaborated on drug screening strategies to investigate the efficacy of 6 screened small molecule drugs targeting P2X7 receptor for metastatic osteosarcoma treatment. Validate one of the drugs as most efficient for therapeutic application.
- Demonstrated effective communication skills through authorship of 7 peer-reviewed papers and presentation of findings at 4 scientific conferences.
Ph.D. Candidate/Senior Research Fellow
Peking University
09.2012 - 03.2019
- Utilized CRISPR-Cas9 shRNA system and live-cell imaging for high-throughput screening of key factors involved in transverse tubule biogenesis during heart development.
- Identified two key factor proteins and associated pathway, serving as potential targets for preventing progression of heart failure.
- Explored CALHM2's function in heart disease through a 3-lab (physiology, structural biology, and clinical medicine) collaboration, uncovering its cardioprotective properties through ROS reduction. This led to a significant 45% enhancement in cardiac performance.
- Proficiently developed heart failure mouse model (TAC), demonstrating expertise in animal handling and in vivo experimental techniques.
Education
Ph.D. - Physiology
Peking University
Beijing, China07.2018
Bachelor of Science - Biotechnology
Minzu University of China
Beijing, China07.2012
Accomplishments
- Led pioneering research on osteocyte Cx43, identifying metabolites promoting osteogenesis
- Established Cx43 as a potential therapeutic target for osteoporosis
- Achieved bone marrow cell architecture analysis by introducing multi-color FACS followed with single cell RNA sequencing
- Validated multiple automated and manual platforms for cell/tissue based gene expression
- Collaborated with team of 3 in the function of CALHM2 in heart disease.
- Uncovered the mechanism of the cardioprotective properties through ROS reduction. Leading a significant 45% enhancement in cardiac performance.
Spearheaded Research on Osteocyte Connexin 43:
Led Groundbreaking Research on CALHM2 Channel in Cardiomyocytes:
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Quote
There is a powerful driving force inside every human being that, once unleashed, can make any vision, dream, or desire a reality.
Tony Robbins
Featured Work
Zhang J*, Riquelme MA, Hua R, Acosta FM, Gu S, Jiang JX. "Connexin 43 Hemichannels Regulate Mitochondrial ATP Generation, Mobilization, and Mitochondrial Homeostasis against Oxidative Stress." Elife. 2022, 11: e82206.
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