Jisha Jayakrishnan

Gritstone bio is a clinical-stage American biotechnology company which develops cancer and infectious disease immunotherapies and vaccines. It was founded in August 2015 as Gritstone Oncology, Inc., and is based in Emeryville, California.

• Perform analytical procedures in support of characterization, stability, and testing of drug substances and products
• Hands-on work experience in different types of assays like CE analysis (Purity analysis of RNA and DNA), Zeta potential determination, and Particle Size Determination (DLS).
• Follows GLP guidelines while performing assays
• Ensure high-quality and timely documentation in established laboratory notebook .Draft characterization analytical reports and certificates of analysis
• Analyze or summarize experimental data and present it at group meetings.
• Perform other duties as assigned, such as lab maintenance, ordering, and stock lab supplies
• Gathered and organized information for research purposes

Boehringer Ingelheim is a research-driven group of companies dedicated to the discovery, development, manufacturing and marketing of innovative health care products.

• Proficiency and experience in running analytical assays and bioassays
• Analyses of in-process samples using analytical techniques Cation exchange chromatography (CEX) Capillary Electrophoreses (CE) and immunobased assays for impurity analysis (ELISA) and qPCR
• Determining concentration of residual proteins such as CHOP and residual protein A by ELISA (Cygnus assay), residual DNA by automated DNA extraction and real-time PCR
• Documented all processes during experiments, keeping meticulous notes of unexpected changes
• Conducted experiments for product development purposes in laboratory settings
• Collaboratively and efficiently work with group to deliver targets within deadlines
• Experience in working with real-time PCR systems and MegMAX automated DNA extraction systems
• Troubleshoot experimental roadblocks and discuss problems with team members to arrive at potential solutions
• Federal Drug Administration (FDA), European Medicines Agency (EMA), and Organization for Economic Cooperation and Development (OECD) establish GLP regulations for human-use drugs. Follow GLP in the Code of Federal Regulations (CFR) Title 21 CFR Part 58 (4) for data analysis, reviewing and storing ELN notebooks based on company policies.
• Ability to work independently, efficiently, and productively in a fast-paced, highly collaborative, and diverse team environment.

• Hands-on-experience with Immunoassays, / IHC (immunohistochemistry), Westernblot, and SDS-PAGE
• Routinely performed immuno-histological staining and procedures for screening of monoclonal antibodies
• Produces average of 300 IHC slides per week to screen for and validate positives during monoclonal antibody development at various stages while managing multiple projects with overlapping deadlines
• Assisted in daily laboratory operations but not limited to maintaining reagent levels, making up of antibodies, dilutions, and daily maintenance of lab equipment
• Perform and assist other assays for characterization of antibodies using different techniques like SDS PAGE and
• Western blot
• Proven ability to organize, prioritize and assist with multiple research projects
• Design and develop immunoassays for known and novel targets, optimize SOPs for new formulations (e.g
• Cocktails, multiplex, pre-diluted ready-to-use format); Participate in validation of antibodies for use in immune assay
• Performed receiving, manufacturing, aliquoting and storing of Monoclonal Antibodies
• Trains new staff and interns in laboratory techniques as well as lab safety and equipment maintenance, as well as documentation procedures
• Assumes responsibility for keeping work area clean, neat and organized
• Completes thorough cleaning at end of work period.