Hi, I’m
Joanna Bueche
Tarpon Springs,Florida
Summary
Accomplished Senior Scientist Cell Biologist with strong project management and interpersonal skills. Track record of designing, optimizing, and validating assays for various applications, including in-vitro diagnostics and reagent manufacturing. Immunogenicity, biomarker, and protein purification projects. Excelling in validation of analytical instruments, creating SOPs, and managing QA/QC documentation. Contributing to startups and CROs, including developing automated diagnostic platforms and modular assays. Bringing over 15 years of experience in immuno-oncology assay development, technology transfer, and project management. Expertise in cell biology, bioassays, and laboratory operations. Successfully translated novel technologies into clinical solutions.
Overview
13
years of professional experience
Work History
Immunologix LaboratoriesTampa, FL
Senior Scientist Flow Cytometry
07.2022 - Current
Job overview
- Lead development and validation of lab instruments for research studies
- Plan and execute instrument qualification, write procedures, and develop study plans
- Conduct studies for multicolor flow cytometry under FDA guidelines
- Organize Flow Cytometry Lab, train staff, and establish lab procedures
- Complete required regulatory and safety training
- Design studies for customer-specific assays like Immunogenicity and Biomarker testing
- Create analysis reports and presentations for samples
- Update clients on project progress, troubleshoot issues
- Collaborate with colleagues to meet project deadlines
- Partner with industry and academic organizations for research collaborations.
Notable LaboratoriesFoster City, CA
Principal Product Development Scientist
01.2021 - 05.2022
Job overview
- Develop clinical applications for fully automated drug sensitivity testing platform
- Design and conduct validation studies for blood cancer testing in line with FDA regulations
- Define product requirements, set technical milestones, and addresses development issues
- Create and maintain user-related documents and procedures
- Coordinate the transfer of technologies from R&D to Product Development and labs
- Process blood and bone marrow samples for testing
- Perform immuno-assays on clinical samples, analyzes data, and reports results
- Manage mammalian cell culture, including cryopreservation and stable cell line engineering
- Supervise junior scientists and associates
- Complete required regulatory and health/safety training
- Collaborate with cross-functional teams to develop diagnostic products contributing to company goals and strategy.
Accellix-Leukodx / Mojave BioSan Carlos, CA
Senior Scientist, Bioassay & IVD Development & Reagent Manufacturing
07.2019 - 12.2021
Job overview
- Develop and conduct specialized bioassays for immune therapies, quality control, and drug release
- Collaborate with R&D teams on custom sample workflows and validation studies
- Write procedures, analyze data, and create reports
- Update assay protocols, enhance workflows, and support clinical development
- Assist in developing cartridge-based flow cytometric assays for cell therapy
- Oversee manufacturing of microfluidics-based diagnostic tools
- Prepare manufacturing and quality control documents, and improve processes
- Lead production of standardized assay reagents for simplified workflows
- Mentor research associates in workflow execution and data analysis.
Kite Pharma/GileadSanta Monica, CA
Sr. Analytical Development Scientist
06.2018 - 09.2018
Job overview
- Develop methods for CAR-T Cell Therapy and drug release analysis
- Assist in designing, optimizing, and troubleshooting various types of assays
- Test samples, reagents, cells, and critical components
- Validate methods for Quality Control lab use.
Bio Vision Inc. (Abcam Co.)Milpitas, CA
Senior Scientist Assay Developer
04.2015 - 05.2018
Job overview
- Develop assays and research tools for Life Science/Drug Discovery
- Create and improve methods, leads quality control, and trains in FACS and Fluorescence Microscopy
- Design and validate cell-based bioassays for various readout methods
- Convert protocols into standard procedures and manage technology transfers
- Write data sheets, guides, SOPs, and quality documentation for reliability
- Manage mammalian tissue culture, including cryopreservation and cell line engineering
- Purify and characterize proteins from various sources, designs bioassays, and conducts stability testing
- Perform protein conjugation, develop assays using click chemistry, and analyze protein interactions
- Utilize molecular and cell biology techniques, such as DNA/RNA manipulation and genetic analysis
- Proficient in multicolor Flow Cytometry, Fluorescence microscopy, and plate reader analysis
- Apply principles of Immunocytochemistry, Immunohistochemistry, and Bioluminescence.
Dept. of Plant and Microbial Biology- UC BerkeleyCA
Postdoctoral Fellow
01.2011 - 01.2015
Job overview
- Assist Professor N
- Louise Glass, supervised undergrad students, and collaborated on bioinformatics
- Conduct genetic analysis of cell death and incompatibility in Neurospora Crassa
- Examine the Neurospora crassa genome to identify kinase targets for cell death, introduced mutations, and studied interactions using genetics and biochemistry.
Education
New Mexico State University
Ph.D.: Biology & Microbiology
Medical University of Warsaw
Master of Science: Pharmacy and Pharmaceutical Sciences
Skills
- Research and Experimentation
- Data Analysis and Interpretation
- Cross-functional Collaboration
- Innovation and Problem-Solving
- Technical Expertise
- Publication and Presentation
- Regulatory Compliance
- Leadership and Mentorship
- Clinical Solutions
- Laboratory Operations
- Client-Facing Environment
- Effective Communicator
- Problem Solver
- Gene Therapy
- Specimen Collection
- Technical Reporting
- Cell Based & Enzymatic Bio-assays
Accomplishments
- Postdoctoral Fellow, Stanford University, 01/2010, 01/2011, Palo Alto, CA
- Research Associate/Laboratory Manager, LSU Health Sciences Center, Dept. of Biochemistry and Molecular Biology, 01/2000, 01/2005, Shreveport, LA
- Product Specialist/Application Scientist - Natural Colors, CHR. Hansen LTD., 01/1998, 01/2000, Warsaw, Poland
Timeline
Senior Scientist Flow Cytometry
Immunologix Laboratories
07.2022 - Current
Principal Product Development Scientist
Notable Laboratories
01.2021 - 05.2022
Senior Scientist, Bioassay & IVD Development & Reagent Manufacturing
Accellix-Leukodx / Mojave Bio
07.2019 - 12.2021
Sr. Analytical Development Scientist
Kite Pharma/Gilead
06.2018 - 09.2018
Senior Scientist Assay Developer
Bio Vision Inc. (Abcam Co.)
04.2015 - 05.2018
Postdoctoral Fellow
Dept. of Plant and Microbial Biology- UC Berkeley
01.2011 - 01.2015
New Mexico State University
Ph.D.: Biology & Microbiology
Medical University of Warsaw
Master of Science: Pharmacy and Pharmaceutical Sciences
Languages
Polish, Native or Bilingual English, Native or Bilingual
Languages
Polish, Native or Bilingual English, Native or Bilingual
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