MaxQuant
Jolene Duda
PhD Student
Minneapolis,MN
Summary
- Scientist with robust background in Mass Spectrometry-Proteomics and Oncology Research with expertise in project conceptualization, optimization, and implementation,
- Detail-oriented team player with strong organizational skills. Ability to handle multiple projects simultaneously with a high degree of accuracy.
- Skilled in multitasking and organization, enhancing team efficiency and advancing scientific discovery.
Overview
8
8
years of professional experience
2
2
Languages
Work History
Intern: Office of Technology Commercialization
University of Minnesota
10.2023 - Current
- Support staff members in their daily tasks, reducing workload burden and allowing for increased focus on higher-priority assignments.
- Assessment the commercial viability of new inventions, review of patent literature, development marketing materials, and identification of potential licenses.
PhD Student: Mass Spectrometry and Proteomics
University of Minnesota
08.2020 - Current
- Label-free, SILAC and TMT quantitative proteomics on ovarian cancer cells, tumor tissue and ascites (Orbitrap/Ultimate 3000 RSLCnano systems).
- Identification of Histone deacetylase (HDAC) 6 protein-interactions through substrate trapping.
- Identification of HDAC inhibitor drug-protein targets by ABPP and PISA chemoproteomics methods.
- Assessment of proteomic alterations occurring in PARP inhibitor resistance development across PDX models
Team Leader: Computer Help Desk
College of Saint Scholastica
09.2016 - 12.2019
- Manage a team of help desk technicians, resulting in increased efficiency and improved service quality.
- Train new employees on help desk procedures, ensuring consistent and high-quality customer support.
- Oversee operations of the Help Desk, and focus on quality assurance goals and improve customer service output.
- Provide frontline hardware and software computer support for faculty, staff, and students. Provide network, academic, and audio-visual support.
Intern: Quality Control Technician
Sappi Paper Mill
06.2019 - 11.2019
- Perform routine accurate and precise testing of specialized cellulose pulp samples.
- Provide accurate test data analysis, labeling, and archiving of samples.
Education
Ph.D. - Biochemistry, Molecular Biology, And Biophysics
University of Minnesota
Minneapolis 05.2001 -
Bachelor of Science - Biochemistry
College of Saint Scholastica
Duluth, MN 05.2001 -
Skills
Mass Spectrometry-Proteomics
Oncology Research
Multitasking and Organization
Attention to Detail
Interpersonal and Social Skills
Affiliations
- Females in Mass Spectrometry (FeMS)
- American Society of Mass Spectrometry (ASMS)
- US Human Proteome Organization (US HUPO)
- Human Proteome Organization (HUPO)
Additional Information
- Chemoproteomics 101: Introduction and Applications, US HUPO Conference (2024)
- Project Management 101, University of Minnesota (2024)
- Introduction to Entrepreneurship, University of Minnesota (2024)
- Leading Across Sectors and Professions for Collective Impact, University of Minnesota (2023)
Timeline
Intern: Office of Technology Commercialization
University of Minnesota
10.2023 - Current
PhD Student: Mass Spectrometry and Proteomics
University of Minnesota
08.2020 - Current
Intern: Quality Control Technician
Sappi Paper Mill
06.2019 - 11.2019
Team Leader: Computer Help Desk
College of Saint Scholastica
09.2016 - 12.2019
Ph.D. - Biochemistry, Molecular Biology, And Biophysics
University of Minnesota
05.2001 -
Bachelor of Science - Biochemistry
College of Saint Scholastica
05.2001 -
Accomplishments
- 2021-2023: Mayo Clinic Ovarian SPORE Developmental Research Project ($75,000)
- 2024-2025: Doctoral Dissertation Fellowship ($25,000)
- 2024: Pletcher Graduate Fellowship ($5,000)
- 2024: Brandenburg Award ($6,000)
Software
Proteome Discoverer
R studio
MSFragger
Perseus
Interests
Co-reviewer: Nature Communications Manuscript Submissions
Committee Member: Department Chair Search Committee
Program Representative: Council of Graduate Students
Graduate student mentor
Relevant Expertise
LC-MS/MS Instrumentation: Agilent 1260 Infinity HPLC, Ultimate 3000 RSLCnano, Q Exactive Plus, Orbitrap Velos, Orbitrap Fusion
Assays: cell viability (sulforhodamine B, MTT), protein quantitation (BCA, Pierce660, Bradford), western blot, HDAC activity
Selected Publications
1. Duda JM and Thomas SN. “Histone Deacetylase 6 with DNA damage repair factors strengthens its utility as a combination drug target in high-grade serous ovarian cancer” (2023) ACS Pharmacology & Translational Science. 6(12) 1924-1933. PMID: 38107255. PMCID: PMC10723650
2. Duda JM, Twigg CAI, and Thomas SN. “Differential histone deacetylase inhibitor-induced perturbations of the global proteomic landscape in the setting of high-grade serous ovarian cancer.” (2022). Proteomics. 23(3-4). PMID: 36193784. PMCID: PMC995782
3. Duda JM and Thomas SN. “Combination of SILAC and substrate trapping for the detection of transient protein interactions.” (2022). Methods in Molecular Biology. Luque-Garcia JL, editor. Springer Nature. Vol. 2603. pp. 219-234. PMID: 36370283
4. Auerbach AA, Becker JT, Moraes SN, Moghadasi SA, Duda JM, Salamango DJ, Harris RS. “Ancestral APOBEC3B Nuclear Localization Is Maintained in Humans and Apes and Altered in Most Other Old World Primate Species” (2022). mSphere. 7(6). PMID: 36374108. PMCID: PMC9769932.