Jonathan Galea

Standard Operating Procedure and Laboratory Analysis

• Performed quantitative LC-MS/MS analysis of 84 pain panel metabolites utilizing validated methodology, maintaining throughput of 400+ patient specimens daily
• Ensured consistent analytical performance while maintaining CLIA and COLA compliance standards
• Validated method performance through systematic evaluation of analytical parameters

Quality Assurance, Control, & Analytical Validation
• Prepared and validated daily calibration curves and matrix-matched quality controls from certified reference materials
• Conducted weekly hydrolysis efficiency testing for enzymatic method validation
• Performed Levie-Jennings statistical analysis for method performance QC analysis
• Coordinated laboratory proficiency testing of toxicology techs through CAP and other third party vendors
• Prepared and validated internal standard solutions for quantitative analysis

Laboratory Quality Management
• Established and maintained quality systems documentation
• Verified equipment calibration and preventative maintenance schedules
• Validated specimen integrity through immunoassay screening protocols
• Maintained chain of custody documentation
• Ensured compliance with hazardous material handling protocols via third party bio-disposal companies

Technical Supervision & Process Implementation
• Provided technical oversight and training for laboratory personnel
• Contributed to technical staff hiring decisions
• Managed inventory and ordering of daily consumables and reference standards
• Assisted in developing and validating new oral fluid testing method

Regulatory Compliance & Inspection Management
• Served as lead technical representative through five successful regulatory inspections (3 CLIA, 2 COLA)
• Maintained complete compliance with all quality and safety standards
• Documented and tracked all inspection findings and corrective actions

Advanced Technical Competencies
• Performed EtG/EtS quantitation via LC-MS/MS for alcohol metabolite analysis
• Executed multiplex qPCR methodology for SARS-CoV-2 detection utilizing 96-well format
• Validated internal standard preparation methodologies
• Maintained analytical instrument calibration verification protocols
• Implemented new testing procedures while maintaining quality standards

• Performed processing and gross evaluation of small tissue biopsies, preparing them for diagnostic interpretation via the pathologists (skin, cervical, gastrointestinal)
• Coordinated and prepared frozen tissue sections, operated and maintained Cryostat and tissue processing equipment (Sakura Tissue-Tek VIP6)
• Assisted in fetal autopsy procedures
• Preparation of complex specimens for gross evaluation ensuring proper fixing, accessioning, and documentation (mastectomies, colectomies, whipple specimen)
• Acted as a liaison between pathologists and the laboratory team, communicating key findings and supporting efficient lab operations

• Processed patient bodily fluids and microscopic samples (e.g., fine needle aspirations, core biopsies, pleural & pericardial effusions, cerebrospinal fluid) for evaluation
• Operation of Leica ST2000 automated system for cell block preparation and specimen staining, both gynecological and non-gynecological
• Operation of ThinPrep Pap smear processors (nightly caseload of approximately60)
• Managed patient case accessioning within EPIC, ensuring proper documentation and tracking of samples
• Ordering laboratory tests, reflexes and add-ons within Michigan Co-Tenancy Laboratories

• Provided tutoring services to over100 GVSU science students in biomedical courses such as biology, microbiology, organic chemistry, and anatomy
• Taught individual and group sessions to reinforce complex concepts, enhancing student comprehension and academic performance
• Organizing and attending monthly meetings via the Student Academic Success Center (SASC) with peer tutors to discuss our experiences and perceived student progress, facilitating a continually-evolving and perceptive learning atmosphere
• Also provided science and test-prep tutoring freelance online through Varsity Tutors