Summary
Overview
Work History
Education
Skills
Work Experience
Conference Presentations
Community Outreach
Positions Of Responsibility
Interests
Timeline
Generic

JOSEPH LLEWELLYN

Suisun,CA

Summary

Meticulous and analytical Researcher enthusiastic about supporting advancements and quality control in biotech industry. Passionate about increasing knowledge and efficiency to drive growth and needed improvements. Adaptive team player with in-depth knowledge of data collection, confidential document control and problem-solving. Ready to apply 5+ years of related experience to challenging new role.

Overview

6
6
years of professional experience

Work History

NIH POSTBAC IRTA FELLOW

BEERMAN LAB, NATIONAL INSTITUTE ON AGING
08.2021 - 07.2023
  • Cultured several induced neural stem cells (iNSCs) derived from progressive multiple sclerosis patients for investigating unique epi- and transcriptomic signatures via single cell RNA/ATAC/multiomic sequencing
  • Processed over 10 human femur head and knee bone samples weekly for HSPC analysis via flowcytometry and compact bone-derived mesenchymal stem cells for culturing via collagenase digestion
  • Troubleshooted and refined proximity ligation assay protocol for analysis of DNA damage machinery recruitment in HSCs
  • Established and refined working protocol for isolation and culture of compact bone-derived mesenchymal stem cells from mouse and human samples, increasing time efficiency by 15% while decreasing use of lab supplies
  • Performed DNA damage analysis using in PBMCs from human blood samples processed by comet assay
  • Trained in utilizing FACS machine, confocal microscope, and Flowjo FACS analysis program for stem/progenitor cells
  • Wrote and refined 10+ lab protocols for producing reagents/buffers, stem cell culturing, and cell isolation techniques
  • Managed and maintained lab orders, cryopreserved cell stocks, and reagents
  • Multi-tasked and worked efficiently to progress several projects from beginning stages
  • Mentored four summer undergraduate interns in basic lab etiquette, aseptic techniques, cell culturing, and creating posters
  • Contributed to research activities by providing specialist input based on professional expertise, practice, experience, and qualifications
  • Collaborated with multidisciplinary team within NIH-NIA to optimize workflow and achieve objectives
  • Maintained accurate records of research findings and provided statistical analysis of data results
  • Contributed to and actively participated in research conception, design and execution to address defined problems

SUMMER RESEARCH ASSISTANT

BARBOZA GARDENER LAB, UC DAVIS
06.2019 - 08.2019
  • Researched and performed in vivo analysis of gut-brain axis on GI function & food intake, and glycan topology of leptin receptor in mice on high and low-fat diets
  • Performed frequent mouse and mouse brain dissections for organ and blood collection used in various studies
  • Diligently maintained mouse colonies, including weening, tagging, food/water replacement, cage cleanup, and cataloguing.

UNDERGRADUATE RESEARCH ASSISTANT

MUDRYJ LAB, UC DAVIS
02.2017 - 06.2019
  • Meticulously performed quality control tests (plasmid construction, agarose gel electrophoresis, western blot, transfections) on FAM111A primers for transfection
  • Managed several human bladder and prostate cancer cell lines for various lab studies
  • Performed drug studies involving already existing drug investigating potential chemotherapeutic effects on human bladder cell lines, by itself and in combination with cisplatin
  • Thoroughly wrote and compiled detailed protocols for several lab techniques/experiments.

Education

Bachelor of Science - Cell Biology

UNIVERSITY OF CALIFORNIA, DAVIS
Davis, CA

NIH POSTBAC IRTA PROGRAM - Aging Biology, Stem Cell Function, And Epigenetics

NATIONAL INSTITUTE ON AGING
Baltimore, MD

Skills

LAB TECHNIQUES

  • QuantitativeQualitative research, experimental design, project Management, scientific Writing, research methodologies, sample testing, writing reports, record management, multitasking abilities
  • Proficient in aseptic cell culture, western blot, flow cytometry, single-cell RNA/ATAC/multiomic sequencing, immunocytochemistry, confocal microscopy, cell surface marker panel development, transfection, drug treatment, comet assay, mini/maxi prep, mouse dissection, primary cell isolation/culture

ANALYTICAL SKILLS

  • Fiji, Image J, CometScore, FlowJo, ZEN Microscopy, and Microsoft Word/Excel/PowerPoint

COMMUNICATION

  • Accurate and concise verbal and written presentation of scientific literature/data

Work Experience

UC Davis Activities & Recreation Center | Davis, CA
Member Service Attendent April 2016 – June 2017

  • Monitored several sections of the primary recreational and fitness center at UC Davis
  • Managed weightlifting and recreational equipment to ensure accurate inventory count and quality of
    use for the safety of patrons
  • Provided leadership to new employees via training and analyzing the establishment’s requirements
    and areas of improvement


UC Davis Academic Assistance & Tutoring Centers | Davis, CA
Tutor – General Chemistry January 2017 – June 2019

  • Managed several college students enrolled in the UC Davis general chemistry course as a group tutor
    and content review instructor
  • Developed contextual and critical thinking approaches for exam preparation and laboratory assignments to
    increase efficiency and efficacy of understanding course material
     Curated workshops involving condensed yet thorough classroom lesson planning and the development of
    online resources containing extensive relevant learning materials and worksheet


Fleet Feet | Davis/Vacaville, CA
Fit Specialist– Outfitter August 2019-July 2021/September 2023-Current

  • Provided quality and holistic services involving educating and guiding customers through an
    efficient fitting process in discovering shoes and products benefitting their needs and wants
  • Developed detailed spreadsheets differentiating shoe brands and models by several variables in
    respect to overall fit, cushioning type & feel, unique features, and support type
    to improve fitting efficiency and lowering product return frequency
  • Led educational walkthrough with customers to set expectations of products
    based on background, needs, and pain/injury related situations
  • Led weekly recovery, nutrition, and running form/mechanics workshops for runners and walkers at
    Fleet Feet Davis during 2019-2021

Conference Presentations

  • NICLOSAMIDE: A POTENTIAL CHEMOTHERAPEUTIC AGENT FOR THE TREATMENT OF BLADDER CANCER, EEOP Summer Poster Symposium, Davis, CA | Sept 2017, UC Davis Cancer Comprehensive Center: Cancer Research Symposium | Sacramento, CA | Sept 2017, UC Davis Undergraduate Research, Scholarship, and Creative Activities Conference | Davis, CA | April 2018
  • CHARACTERIZATION OF AN ANDROGEN RECEPTOR REGULATED TRANSCRIPT FAM111A IN PROSTATE CANCER, EEOP Summer Poster Symposium | Davis, CA | August 2018
  • SINGLE CELL MULTI-OMIC ANALYSIS OF MULTIPLE SCLEROSIS-DERIVED INDUCED NEURAL STEM CELLS, EVALUATING THE PRESENCE OF SENESCENCE IN INDUCED NEURAL STEM CELLS DERIVED FROM PROGRESSIVE MULTIPLE SCLEROSIS PATIENTS, NIH Virtual Postbac Poster Day | NIH Bethesda, MD | April 2022 & 2023, CSHL Mechanisms of Aging Conference | Cold Spring Harbor, NY | Sept 2022

Community Outreach

  • Curated interactive science workshops pertaining to global climate change (I.e., ocean acidification) for students in the Lower Lake High School Upward Bound program (2015-2017)
  • Organized clinics and provided healthcare with Volunteers Around the World for disadvantaged communities in areas surrounding Bocas del Toro, Panama (2016)
  • Volunteered at Shifa Community Clinic to fulfill clinic positions to provide quality and free healthcare to the medically disenfranchised communities in Sacramento, CA (2018)
  • Fervently motivated runners whilst providing course guidance, support, and traffic control for the Davis Stampeded running race (2020)
  • Collected and delivered donated food items for Manna House Inc for providing meals to the homeless and poor in Balitmore, MD (2021-2022)

Positions Of Responsibility

  • UC DAVIS ACADEMIC ASSISTANCE & TUTORING CENTERS TUTOR, 01/2017, 06/2019, Worked within UC Davis as a tutor and content review instructor for the general chemistry course, Contributed towards developing effective and thorough learning materials for the general chemistry course whilst tutoring/teaching students in a one-on-one or group environment, Lead an extensive content review course and created effective and thorough study materials for students
  • SOCIAL COORDINATOR/PRESIDENT OF LEADERS OF ACHIEVEMENT IN BIOLOGICAL SCIENCES, 05/2017, 06/2019, Lead a team of board members to build a strong and welcoming community for and cater to the needs of STEM students in the UC Davis Biological Undergraduate Scholars Program (BUSP), Empathized with my board members and student community to deliver effective solutions/ideas to concerns and situations addressed
  • VOLUNTEERS AROUND THE WORLD OUTREACH TEAM LEADER, 08/2016, 06/2017, Curated informational and recruitment events to encourage students who relish in participating in medical outreach missions

Interests

Running & Injury Experience: Within Fleet Feet, provided educational insight into the background,
remedies, and products sold by Fleet Feet relating to numerous individuals (mostly elderly) with
complex and severe feet and/or leg-based injuries/ailments (Ie, plantar fasciitis, Morton's neuroma,
knee surgery, lower back pain, shin splints, bone spurs, bunions, tendonitis, etc), as well as unique foot
shape/sizes


Personal Interests: Endurance sports (running, open water swimming, triathlon), avid reader, running
shoes engineering technology, nutrition, healthy aging

Timeline

NIH POSTBAC IRTA FELLOW

BEERMAN LAB, NATIONAL INSTITUTE ON AGING
08.2021 - 07.2023

SUMMER RESEARCH ASSISTANT

BARBOZA GARDENER LAB, UC DAVIS
06.2019 - 08.2019

UNDERGRADUATE RESEARCH ASSISTANT

MUDRYJ LAB, UC DAVIS
02.2017 - 06.2019

Bachelor of Science - Cell Biology

UNIVERSITY OF CALIFORNIA, DAVIS

NIH POSTBAC IRTA PROGRAM - Aging Biology, Stem Cell Function, And Epigenetics

NATIONAL INSTITUTE ON AGING

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JOSEPH LLEWELLYN