Joshua Serratos

Manufacture of Catalog and Custom mRNA:

• Produced both catalog and custom mRNA sequences, tailored for specific research and therapeutic applications.

DNA Work (PCR and Digest):

• Performed PCR (Polymerase Chain Reaction) to amplify targeted DNA sequences, producing millions of copies of specific DNA segments.
• Conducted DNA digestion using restriction enzymes to cleave DNA at specified sequences for cloning, analysis, and molecular biology applications.

DNA Purification and Final Fill:

• Isolated DNA from biological materials, removing proteins, lipids, and contaminants.
• Aliquoted purified DNA into final containers under sterile conditions for storage or distribution.

In Vitro Transcription (IVT):

• Synthesized RNA molecules from DNA templates using IVT, producing mRNA for research and therapeutic applications.

mRNA Purification (RNeasy Columns):

• Purified mRNA using RNeasy spin columns, leveraging silica membrane technology to selectively bind RNA and remove impurities.

Oligo Production on ÄKTA Avant and Diafiltration TFF:

• Produced oligonucleotides (short DNA/RNA sequences) using ÄKTA Avant chromatography systems for purification.
• Applied Diafiltration TFF to concentrate and desalt macromolecule solutions, ensuring high-quality RNA/protein preparations.

mRNA Final Fill:

• Aliquoted purified mRNA into final containers, ensuring sterility and stability for storage or downstream applications.

DNA and RNA Gel Electrophoresis:

• Performed gel electrophoresis to separate and analyze DNA/RNA fragments by size, ensuring purity and integrity of nucleic acids.

Technical Review of Batch Records:

• Conducted technical reviews of batch records to ensure compliance with production protocols and adherence to quality standards.

Running Experimental Projects:

• Designed and executed experiments to address scientific questions, develop new techniques, and create innovative products.
• Analyzed data, interpreted results, and reported findings for further optimization and product development.

HPLC (High-Performance Liquid Chromatography):

• Utilized HPLC to separate, identify, and quantify nucleic acids, proteins, and other biomolecules, ensuring high purity and concentration in samples.

• Delivered exceptional customer service by ensuring each guest had a positive experience, addressing inquiries, and resolving concerns in a timely and professional manner.
• Worked efficiently in a fast-paced, high-volume environment while maintaining a focus on quality, accuracy, and consistency in all tasks.
• Fostered a team-oriented atmosphere by collaborating with coworkers, supporting colleagues during peak hours, and promoting a positive work culture.
• Ensured meticulous attention to detail and quality in every aspect of the job, including food preparation, cleanliness, and order accuracy, aligning with In-N-Out’s high standards of service.
• Demonstrated strong communication skills, both with customers and team members, ensuring a smooth workflow and effective problem-solving under pressure.
• Maintained a clean and organized work area, adhering to food safety and sanitation standards while contributing to the overall operational efficiency of the store.
• Trained new and existing team members, enhancing overall performance, ensuring adherence to company standards, and promoting a collaborative work environment.