Summary
Overview
Work History
Education
RESEARCH INTERESTS
Conferences and Presentations
Profession Relevant Skills
HOBBIES AND INTERESTS
Timeline
Generic

Juan Claudio Escalante

Lawrence,KS

Summary

As a student with a biology background, I am transitioning towards bioengineering, having started a Master's program in the field. My past research includes projects on microfluidic particle separation and neuroscience experiments involving cognitive tests in rats and tissue sectioning parts of the rat brain. I aim to soon start working on biosensors, combining my biology expertise with engineering skills. After graduate school, I am particularly interested in pursuing a career in industry, where I can apply my research and technical skills to practical, real-world challenges.

Overview

1
1
year of professional experience

Work History

Purdue University’s 2023 Summer Research Program

Supervisor: Tamara Kinzer-Ursem, PhD
06.2023 - 07.2023
  • Project focused on using inertial microfluids to separate particles of varying sizes within a microchip.
  • Two phases: microchip fabrication and particle imaging.
  • Fabrication: PDMS was deposited onto a mold using photolithography, followed by 4-hour thermal curing at 65°C.
  • PDMS demolded, reshaped with precise inlet/outlet ports, and bound to a glass substrate using plasma treatment to create chip.
  • Particle imaging: 1 µm, 7.32 µm polystyrene, and 20 µm acrylic particles analyzed using a fluorescence microscope.
  • A syringe pump controlled flow rates of 0.10 to 5.0 ml/min.
  • Outcome: Successfully trapped 20 µm particles and separated 1 µm and 20 µm particles, confirming method's efficacy.

Independent Study

Supervisor: David Daberkow, PhD
04.2023 - 06.2023
  • Sought permission from Dr. Daberkow to continue the Senior Capstone experiment as an independent study (see below for more detail).
  • Extended rat treatment for a total of 11 weeks.
  • Administered another Novel Object Recognition (NOR) test after the treatment phase.
  • Conducted brain extraction following the extended treatment.
  • Stored all collected brain samples at -20˚C to preserve integrity

Senior Capstone: Learning and Memory

Supervisor: David Daberkow, PhD
01.2023 - 03.2023
  • Study involved 24 male Sprague Dawley rats divided into three groups: fructose, caffeine, and control (water).
  • Rats received either 0.3 g/L caffeine, 10% fructose solution, or water for two weeks.
  • Then came the three experimental phases: habituation (5 minutes/day for 4 days), familiarization (10 minutes with two identical objects), and test (5 minutes with a novel and familiar object 24 hours later).
  • Camera recorded interactions; fructose group had the highest cognitive improvement (PI score: 65.7), followed by caffeine (62.1) and control (52.7).
  • Outcome: ANOVA test showed non-significant results (p = 0.217867) due to short treatment duration.

TRIO Ronald E. McNair Scholars Research Internship

Supervisor: David Daberkow, PhD
06.2022 - 08.2022
  • Recorded dopamine levels from rat brains using microelectrodes.
  • Passed a current of 300 volts for 45 seconds to create an electrolytic lesion around the microelectrode tip.
  • Euthanized the rat and removed the brain.
  • Flash froze the brain for 10 seconds using liquid nitrogen, then stored it at -20°C.
  • Affixed brains onto a cryostat chuck with mounting medium and sliced them into 12 µm coronal sections using a cryostat machine.
  • Stained sections and added Permount for protection.
  • Outcome: Identified the targeted area within the striatum under a microscope for investigation.

Neuroscience Research Assistant

Supervisor: David Daberkow, PhD
01.2022 - 06.2022
  • Assisted in the optimization of laboratory protocols, leading to increased efficiency and improved experimental outcomes.
  • Created electrodes for Dr. Daberkow to record dopamine in rats: prepared silica and carbon fiber, threaded them together, applied epoxy, attached a dip pin, and tested with FSCV, trimming as needed.

Education

Master of Science - Bioengineering

University of Kansas
Lawrence, KS
05.2026

Bachelor of Science - Biology

Eastern Washington University
Cheney, WA
06.2022

Associate of Science - Biology

Columbia Basin College
Pasco, WA
06.2020

RESEARCH INTERESTS

  • Molecular, Cell and Tissue Engineering
  • Biomaterialsand Regenerative Engineering
  • 3D bioprinting and Microfluidics


Conferences and Presentations

  • 2023 Murdock College Science Research Conference (November 2023)

Escalante, J.C., Bergan, L., Gilchrist, & Daberkow, D. (2023). The effects of fructose and caffeine on novel object recognition in male Sprague Dawley rats. Poster presentation at the annual Murdock Conference. Vancouver, Washington.

  • 2023 SACNAS Conference (October 2023)

Escalante, J.C., & Kinzer-Ursem, T. (2023). Particle Separation: Harnessing Inertial Microfluids for PreciseParticle Sorting. Poster presentation at the annual SACNAS conference. Portland, Oregon.

  • 2023 Biomedical Engineering Society Conference (BMES) (October 2023)

Escalante, J.C., & Kinzer-Ursem, T. (2023). Particle Separation: Harnessing Inertial Microfluids for PreciseParticle Sorting. Poster presentation at the annual BMES conference. Seattle, Washington.

  • 2023 Purdue Summer Research Symposium (July 2023)

Escalante, J.C., & Kinzer-Ursem, T. (2023). Particle Separation: Harnessing Inertial Microfluids for PreciseParticle Sorting. Poster presentation at the annual Purdue SROP Symposium. West Lafayette, Indiana.

  • 2023 EWU Research Symposium (May 2023)

Escalante, J.C., Bergan, L., Gilchrist, N., Farias, A., Badillo, C.S., Kuplack, C., Kakou, K., Wawa, E., & Daberkow, D. (2023). The effects of fructose and caffeine on novel object recognition in male Sprague Dawley rats. Poster presentation at the annual EWU Research Symposium. Cheney, Washington.

  • 2022 EWU McNair Summer Research Symposium (August 2022)

Escalante, J.C., & Daberkow, D. (2022). Determining Dopamine Microelectrode Placement in the RatBrain. Oral presentation at the annual 2022 EWU McNair Summer Research Symposium. Cheney, Washington.

  • 9th Annual McNair PNW PhD Prep Conference (July 2022)


Profession Relevant Skills

  • Microbiology skills: Brightfield and phase contrast microscopy, Streak agar plates, Streak TSA slants, Gram stain, Endospore stain, Phage titration, Serial dilutions
  • Organic Chemistry skills: TLC, IR Spectroscopy, Mass Spectroscopy, Gas Chromatography, NMR, Distillations, Extractions
  • Neuroscience skills: Rat Behavioral testing, Brain extraction, Electrode fabrication, Tissue sectioning, Data analysis
  • Microfluidic skills: Microchip fabrication, Particle imaging, Flow rate control, Particle separation
  • SolidWorks skills: 3D modeling, Technical drawings, Assembly design
  • Excellent attention to detail and incredible organizational skills, which keep me focused on the small parts of each experiment.
  • Dedicated to performing experiments safely and following all of the proper regulations to ensure reliability.

HOBBIES AND INTERESTS

When I’m not in the lab, I love staying active. Whether it’s playing soccer, shooting hoops, or hitting the slopes on my snowboard in the winter, I’m always up for something fun. I also enjoy trying new things whenever I can. And if I can't do those, I still try to hit the gym to keep the energy going.

Timeline

Purdue University’s 2023 Summer Research Program

Supervisor: Tamara Kinzer-Ursem, PhD
06.2023 - 07.2023

Independent Study

Supervisor: David Daberkow, PhD
04.2023 - 06.2023

Senior Capstone: Learning and Memory

Supervisor: David Daberkow, PhD
01.2023 - 03.2023

TRIO Ronald E. McNair Scholars Research Internship

Supervisor: David Daberkow, PhD
06.2022 - 08.2022

Neuroscience Research Assistant

Supervisor: David Daberkow, PhD
01.2022 - 06.2022

Master of Science - Bioengineering

University of Kansas

Bachelor of Science - Biology

Eastern Washington University

Associate of Science - Biology

Columbia Basin College
Juan Claudio Escalante