Juliette Estrada

Cellular Biology Lab (Production and Purification of Recombinant GST-DHFR-HIS Fusion Protein with E. Coli, BL21 strain)

• Proficient in Pipette application and maintenance
• Experienced use with standard centrifuges and microcentrifuge
• Bradford Assay
• GENESYS spectrophotometer
• SDS PAGE analysis
• Western Blot analysis

Genetics Lab (Investigation of Drosophila melanogaster’s Sepia Mutation)

• Expasy translation tools software
• Conducted experimental procedures with live specimens (Drosophila melanogaster)
• Investigation of Drosophila melanogaster’s Sepia Mutation
• Experience in DNA genomic extraction from Drosophila melanogaster species.
• Experience in PCR amplification procedure.
• Miniprep of plasmid DNA procedure
• Gel Image Analysis
• Practiced safety regulations in handling and disposing of Drosophila melanogaster specimens.

Organic Chemistry Lab

• EHS (Environmental, Health Safety) Compliance
• PPE and laboratory safety Compliance
• Operated IR Spectrometers
• Manage H-NMR Spectrometer.
• Column Chromatography
• Simple and fractional distillation
• Practiced wet/dry lab safety regulations.
• Cell culture maintenance
• IMAC method (affinity tagged protein purification)

• Helped manage the student appointment calendar of the Department Advisors.
• Organized and created department courses availability using Excel spreadsheets.
• Assisted in department purchases by creating purchasing orders.
• Answer phones to help students, faculty, and staff with questions about the department activities, general information, and appointments.
• Distributed informational flyers for volunteering and study abroad programs and other educational opportunities.
• Filed and sorted correspondence and department documents.
• Sorted and channeled email correspondence to staff members and faculty.

• Represented educational program at meetings and conferences to serve as subject matter liaison for array of services and activities assigned.
• Responsible for monitoring student’s progress during the lecture hours.
• Assessed student’s attendance and participation during lectures hours.
• Maintained communication with the professor in charge of the course.
• Followed course curriculum to support students.
• Maintained office hours to provide one-on-one support for students in person or through Teams

• Supported navigators in outreach efforts to assist low-income families in accessing affordable insurance options.
• Organized information in an accessible, easy-to-understand format, ensuring clarity for families navigating complex insurance details.
• Collected contact information for follow-up, enhancing the program’s ability to maintain future connections with participants.
• Distributed educational materials at outreach events, increasing community awareness and understanding of available insurance resources.