Julion Landry

Dedicated and hardworking individual, passionate about making a positive impact on the world. Experienced in the biotech field, I possess a strong ability to overcome obstacles and find innovative solutions. My problem-solving skills and proactive approach enable me to confidently tackle new challenges. Recognized for adaptability, creativity, and a results-oriented mindset, I am committed to driving organizational success through meaningful contributions. Proficient, skilled in collecting and interpreting data obtained through experimentation. Maintaining detailed and comprehensive laboratory notebooks, and keeping operations in full compliance with standards.

• Buffer Preparation and pH Calibration:Creating different buffer systems (Tris, PBS) and calibrating a pH meter with multi-point calibration buffers.
  
• DNA Replication and Repair Mechanisms:Study of DNA polymerases, ligases, and helicases in DNA replication and repair pathways (e.g., mismatch repair, nucleotide excision repair).
  
• Biomedical Ethics:Discussion on gene editing ethics (e.g., CRISPR-Cas9), human cloning, and genetic data privacy in personalized medicine.
  
• Southern and Northern Blotting:Performing Southern blotting (for DNA) and Northern blotting (for RNA) with radio-labeled probes for gene detection.
  
• Capillary Electrophoresis:Use of capillary electrophoresis for analyzing DNA fragments and sequencing reaction products.
  
• Gas Chromatography:Using gas chromatography to separate and analyze volatile compounds from biological samples.
  
• Atomic Absorption Spectroscopy (AAS):Used for determining metal ions in biological samples instead of UV/Vis spectrophotometry.
  
• Ultracentrifugation:Separating macromolecules (proteins, nucleic acids) using high-speed ultracentrifugation based on density gradients.
  
• Protein Quantification by Bicinchoninic Acid (BCA) Assay:Alternative to Bradford assay, using BCA assay for more accurate protein concentration determination.
  
• Ion-Exchange Chromatography:Separation of proteins and nucleic acids based on charge instead of gel filtration chromatography.
  
• Bacterial Fermentation and Growth Monitoring:Use of bioreactors for bacterial fermentation, cell density monitoring, and sampling instead of simple culturing.
  
• Acid-Base Equilibria in Buffer Design:Understanding the Henderson-Hasselbalch equation for precise pH control in buffer systems.
  
• Differential Scanning Calorimetry (DSC):Using DSC to measure the stability and denaturation of biomolecules through heat absorption.
  
• Statistical Process Control (SPC) in QA/QC:Employing SPC techniques for monitoring and controlling bioprocesses within cGMP-compliant manufacturing.
  
• Downstream Purification using Tangential Flow Filtration (TFF):Using TFF for concentration and buffer exchange during protein or viral particle purification.
  
• Sterilization by Autoclaving and Validated Procedures:Sterilizing equipment and media using autoclaving protocols, followed by sterile gowning techniques.
  
• Common Analytical Equipment in Biotechnology:Familiarity with analytical instruments like mass spectrometry (MS), HPLC, and bioreactors.
  
• Liquid Chromatography–Mass Spectrometry (LC-MS):Using LC-MS for peptide and protein identification instead of FPLC.
  
• Confocal Microscopy:Employing confocal microscopy for high-resolution imaging of cells and subcellular structures instead of fluorescence microscopy.
  
• Quantitative Real-Time PCR (qPCR):Using qPCR for quantitative analysis of DNA and gene expression levels rather than traditional NanoDrop measurements.
  
• Next-Generation Sequencing (NGS):Utilizing high-throughput NGS techniques for whole-genome sequencing instead of basic gene sequencing.
  
• Pipetting Techniques with Electronic Pipettes:Performing precise dilutions and sample handling with electronic or multichannel pipettes.
  
• Process Development and Optimization:Focusing on process development for bioprocess scale-up in research and development (R&D) settings.
  
• Risk-Based Quality Management Systems (QMS):Implementing risk-based approaches in QMS for regulatory compliance and ensuring SOP adherence.
  
• Good Documentation Practices (GDP):Emphasizing accuracy and compliance in laboratory notebooks, batch records, and document control.
  
• Protein Engineering and Mutagenesis:Modifying protein sequences using site-directed mutagenesis and studying protein structure-function relationships.
  
• Microbial Growth :Maintaining bacterial cultures in chemostats for continuous culture and metabolic studies.

INSTRUMENTS INLCUDING:

• Spectrophotometer (Nanodrop)
• Spectrophotometer (Lambda)
• Plate Reader
• Fluorescent Microscope
• Fluorometer
• qPCR
• Fast Protein Liquid Chromatography (FPLC)
• High Pressure Liquid Chromatography (HPLC)
• Gas Chromatography (GC)