Karthik Swaminathan
Laramie,WY
Summary
Graduate student with a strong background in protein expression, purification, and characterization techniques.
Overview
10
10
years of professional experience
1
1
Certification
Work History
Graduate Research Assistant
Department of Zoology and Physiology
Laramie, WY
10.2023 - Current
- Drafted the IACUC protocol and implemented a comprehensive Unpredictable Chronic Mild Stress experiment plan for studying animal models of depression in mice.
- Performed mice brain extraction and polysome profiling of mouse brain.
- Presented lab's work at the NISBRE 2024 conference in Washington, D.C.
- Contributed to the setup of a functional laboratory environment.
Dissertation Student (M.Tech)
IIT Guwahati
Guwahati, Assam, India
06.2021 - 04.2022
Final year M.Tech project was titled "Expression and purification of Hepatitis B Virus Core protein (Cp) and monitoring its' assembly into viral capsids using steady state fluorescence
anisotropy."
Project Assistant
Institute of Microbial Technology
Chandigarh, India
09.2015 - 04.2016
Under the guidance of Dr. Charu Sharma, contributed to a research project involving purification of Mycobacterium tuberculosis virulent protein (Erp) and characterization of novel mutations within specific virulent genes associated with second line drug resistance.
Dissertation Student (B. Tech)
Center for Cellular and Molecular Biology
Hyderabad , Andhra Pradesh, India
12.2014 - 04.2015
Worked under the guidance of Dr. Madhusudhana Rao to complete my B.Tech Biotechnology final year research project centered around the expression, purification, and characterization of thermostable variants of firefly (Photinus pyralis) luciferase.
Education
Ph.D. - Neuroscience
University of Wyoming
Laramie, WYMaster of Technology - Biotechnology
IIT Guwahati
Guwahati, India06-2022
Bachelor of Technology - Biotechnology
Anna University
Chennai, India04-2015
High School Diploma -
D.A.V Senior Secondary School
Chennai, India03-2008
Skills
Laboratory Skills
- Protein expression and purification from bacterial systems
- Site-directed mutagenesis
- Western Blot analysis
- Chromatography (size exclusion, ion-exchange, affinity)
- Spectroscopy (UV-Vis, Fluorescence, CD)
- Sanger sequencing
Computational Skills
- R Programming
- Python
Certification
- IACUC mandated certifications at University of Wyoming for working with mice.
Publications
- Alom, S. E., Swaminathan, K., Nuzelu, V., Singh, A., de Rocquigny, H., & Swaminathan, R. (2024). Label-Free Tracking of Hepatitis B Virus Core Protein Capsid Assembly in Real-Time Using Protein Charge Transfer Spectra. Biomacromolecules, 10.1021/acs.biomac.4c00521. Advance online publication. https://doi.org/10.1021/acs.biomac.4c00521
Timeline
Graduate Research Assistant
Department of Zoology and Physiology
10.2023 - Current
Dissertation Student (M.Tech)
IIT Guwahati
06.2021 - 04.2022
Project Assistant
Institute of Microbial Technology
09.2015 - 04.2016
Dissertation Student (B. Tech)
Center for Cellular and Molecular Biology
12.2014 - 04.2015
Ph.D. - Neuroscience
University of Wyoming
Master of Technology - Biotechnology
IIT Guwahati
Bachelor of Technology - Biotechnology
Anna University
High School Diploma -
D.A.V Senior Secondary School
- IACUC mandated certifications at University of Wyoming for working with mice.
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