Keegan Hart
Narragansett,RI
Summary
Focused Laboratory professional bringing 4 years of research, reporting and experiment development. Well-versed in enforcing laboratory safety to cultivate safe and productive work environments. Proficient in R with demonstrated success in monitoring and observing experiments to analyze and report findings.
Overview
7
7
years of professional experience
Work History
Biological Science Technician
ARS Shellfish Genetics Laboratory
Kingston, RI
11.2021 - Current
- Perform the logistical planning, record keeping, and sample collection for a large-scale population genomics project consisting of 2000 oysters from 7 states and 44 separate populations
- Develop and test protocols to extract DNA and RNA from oyster swab and biopsy samples
- Create and update protocols, SOP’s and Job Hazard Analyses
- Serve as purchase card holder for 8 person working group
- Organize and submit large procurement packages (>100K)
- Maintain inventories for all items across three laboratory locations
- Train and supervise Research Assistants, Graduate Students, and Undergraduates
- Assist with oyster field sampling
Part Time Faculty
URI College of the Environment and Life Sciences
Kingston, RI
09.2021 - 12.2021
- Taught two sections of BIO 103
- Assisted with preparations for weekly TA meetings.
Research Assistant I (salaried, USDA Grant-funded Position)
ARS Shellfish Genetics Laboratory
Kingston, RI
07.2020 - 11.2021
- Assist in the design and implementation of experimental protocols.
- Organize and analyze large data sets using R and R Studio
- Assist with sequencing library preparation for 144 samples
- Maintain 2,000 oysters in laboratory flow-through systems
- Execute shellfish disease diagnostics and tissue sample collection
- Perform water quality monitoring and maintenance of wet laboratory.
STEM Tutor
URI Academic Enhancement Center
Kingston, RI
01.2018 - 05.2020
- Tutor groups of 2-8 students in advanced Chemistry and Physics classes on a weekly basis.
URI Biology Lab Assistant Manager
URI College of the Environment and Life Sciences
Kingston, RI
05.2017 - 05.2020
- Assisted in the creation of new laboratory experiments and lessons
- Supervised three undergraduate assistants
- Prepared the laboratory spaces weekly for 3 different introductory Biology courses
- Maintained all laboratory equipment.
URI Undergraduate Teaching Assistant
URI College of the Environment and Life Sciences
- Answered student questions and taught lecture during 3-hour weekly lab for four sections of BIO 103 and 104.
Education
B.S. in Biological Sciences -
University of Rhode Island
Skills
- qPCR
- DNA and RNA extraction
- Sequencing library preparation
- Gel electrophoresis
- Microscopy
- Spectrophotometry
- R and R Studio
- QIIME2
- Mothur
- CLC Genomics Workbench
- DESeq2
- WGCNA
- Plink
Publications
Proestou, D. A., Sullivan, M. E., Lundgren, K. M., Ben-Horin, T., Witkop, E. M., & Hart, K. M. (2023). Understanding Crassostrea virginica tolerance of Perkinsus marinus through global gene expression analysis. Frontiers in Genetics, 14, 1054558. https://doi.org/10.3389/fgene.2023.1054558
Trainings
- BD FACSymphony A1 Operator Course
- Keck Graduate Institute Clinical Genetics and Bioinformatics Summer Program.
Timeline
Biological Science Technician
ARS Shellfish Genetics Laboratory
11.2021 - Current
Part Time Faculty
URI College of the Environment and Life Sciences
09.2021 - 12.2021
Research Assistant I (salaried, USDA Grant-funded Position)
ARS Shellfish Genetics Laboratory
07.2020 - 11.2021
STEM Tutor
URI Academic Enhancement Center
01.2018 - 05.2020
URI Biology Lab Assistant Manager
URI College of the Environment and Life Sciences
05.2017 - 05.2020
URI Undergraduate Teaching Assistant
URI College of the Environment and Life Sciences
B.S. in Biological Sciences -
University of Rhode Island
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