Keerti Upadhyay

• Prepared SOPs, quality manual, safety manual and all other regulatory related documentation activities.
• Implemented a safety Laboratory environment in compliance with good practices and applicable regulations.
• Performed tests & Instrument Validations in the department.
• Performed all QC related activities with maintaining Quality control & Assurance documentation in the department.
• Contributed to an atmosphere of teamwork in the laboratory.
• Assisted in Molecular Biology Lab setup.
• Extraction of M. tb. DNA from sputum sample.
• Amplification of extracted DNA Through PCR.
• Testing Genotypic drug susceptibility (Rifampicin and Isoniazid)of Mycobacterium tuberculosis through Hain Assay.
• Estimation of DNA through Nanodrop and Electrophoresis.
• Mycobacterium tuberculosis Complex identification through Accuprobe.

• DNA and RNA extraction from various human tissues including blood and serum samples for daily molecular testing and ensures correct patient labeling.
• Performed molecular techniques such as real-time PCR, primer designing and gel electrophoresis.
• Prepared Corrective and Preventive Action (CAPA) documents for nonconformance events observed during the pre-analytical, analytical, and post-analytical clinical laboratory testing.
• Developed and implemented training initiatives for new hires.
• Developed and implemented preventive maintenance procedures.
• Documented all work with 100% accuracy in a timely manner. Recorded associated information (lot numbers, dates, data, etc.) on standard documents while following good laboratory Practices.
• Maintained laboratory equipment, including completing logs and maintenance, i.e. clean, sterilize, maintain, and shut down laboratory equipment, accessories, and instruments used in research experiments, daily and weekly as required.
• Unpacked reagent orders as received; logs them in the reagent database; creates labels, tracking documentation, and QC forms; and stores them in the proper location and conditions.
• Tracked, entered, and monitored patient files using the Laboratory Information System.
• Assisted to set up Molecular biology lab at different location.

June 2007 – August 2007
Various techniques and protocols for the Isolation of human genomic DNA and other molecular techniques at Human DNA Bank, IQRA Biotech, Lucknow, India.

April 2008 – May 2008
Research project entitled”Genomics of drug resistant Mycobacterium tuberculosis” under the supervision of Dr. A.N. Vats, Scientific Director of Atharva Biosciences New Delhi, India.

• Certification in French Language from Amity School of Foreign Language during 2006-2008.
• Certification in English and Business Communication from Amity Institute of English and Business Communication during 2006-2008.
• Certification in Behavioural Science from Amity Institute of Psychology and Allied Sciences during 2006-2008.
• Certification in Good Laboratory Practices from Achievement Labs Management Systems Institute, Greater on 14 Feb 2011.
• Completed the training program on Molecular techniques involved in performing the Genotype MTBDR plus line probe assay and system operations on the TWINCUBATOR (HAIN LIFESCIENCES, GmbH)
• Completed the training program on Molecular techniques involved in performing the Accuprobe M. tuberculosis complex culture identification test and system operations on the GENPROBE instruments.