Kelli Burley
Woodinville,WA
Overview
10
10
years of professional experience
Work History
Scientist, Oncology Discovery Immunology
Pfizer
01.2024 - Current
- Develop new in-vitro assays to interrogate novel payloads on toxicity to immune cells
- Design co-culture assays to investigate payload induced immune activation
- Participate in project team goal setting, timelines, and governance meetings
Research Associate III, Immunology
Seagen
06.2021 - 12.2023
- Design and optimize cell based assays to investigate novel cancer targets
- Develop standardized in vitro methods for interrogating novel and known immuno-oncology therapeutics
- Support projects for submission of investigational new drug (IND) application by validating mechanism of action and writing experimental reports
- Present research findings to a diverse audience including formal poster presentation for company wide conference
Research Associate II, Experimental Immunology
Allen Institute for Immunology
09.2019 - 05.2021
- Developed 28 color T cell focused panel on the Cytek Aurora
- Designed in vitro experiments to interrogate a potential B cell biomarker for Rheumatoid Arthritis
- Performed assays to support flow cytometry panel development such as T cell differentiation cultures, MSD, and intracellular flow cytometry
- Optimized and supported ongoing pipeline efforts such as PBMC thaw, protocol optimization, and SOP writing
Contract Research Associate, Immunology
Alpine Immune Sciences
11.2017 - 09.2019
- Analyzed binding targets using flow cytometry
- Performed cytokine analysis using ELISA and Magpix
- Maintained, phenotyped, and sorted cell lines
Research Associate I, Vaccine and Infectious Disease Division
Fred Hutch
07.2015 - 03.2017
- Performed ICS and ELISpot in accordance with GCLP standards
- Assisted in development of B-cell ELISpot assay for use in future clinical trials
- Analyzed high throughput data using flow cytometry, FlowJo, Prism, CTL analyzer, and Immunospot software
- Assisted with laboratory maintenance including equipment upkeep, BL2+ biohazard management, and preparation of required reagents
Education
Bachelors of Science - Biochemistry
Seattle Pacific University
Seattle, WA03.2015
Shoreline Community College
Shoreline, WA06.2012
Clark University
Worcester, MA05.2010
Skills
MULTIPARAMETER FLOWCYTOMETRY
- 20 color flow cytometry panel development
- Flowjo
- Prism
- Intracellular cytokine staining
- Cell sorting
CELL BASED ASSAYS
- Immune co-culture assays
- Immunotox assays
- Mixed Lymphocyte Reaction
- SEB Assay
- Luminex
- CEF/CEFX stimulation assay
- Bead based stimulation assays
- NK Killing assays
- Antibody internalization
- MSD
- Cell titer glow assay
- ELISA
- ELISpot
INSTRUMENTATION
- Cytek Aurora
- BD Symphony
- Thermo Fisher Attune
- BD LSDII
- Incuctye
- Envision
- Octo dissociator
- Sony Cell Sorter
CELL HANDLING
- PBMC isolation
- Magnetic bead isolation
- Macrophage differentiation
- T cell differentiation
- Dissociated tumor cells
- Splenocytes
BINDING ASSAYS
Publications
-Multimodal analysis for human ex vivo studies shows extensive molecular changes from delays in blood processing, iScience, Volume 24, Issue 5, 2021,. Adam K. Savage, et al.
-"PF-08046032: A Novel, Investigational CD25-Directed Antibody–Drug Conjugate Optimized for Selective Depletion of Regulatory T Cells in Advanced Malignant Tumors." Molecular Cancer Therapeutics (2025): OF1-OF13. Abdelhamed, Sherif, et al.
Timeline
Scientist, Oncology Discovery Immunology
Pfizer
01.2024 - Current
Research Associate III, Immunology
Seagen
06.2021 - 12.2023
Research Associate II, Experimental Immunology
Allen Institute for Immunology
09.2019 - 05.2021
Contract Research Associate, Immunology
Alpine Immune Sciences
11.2017 - 09.2019
Research Associate I, Vaccine and Infectious Disease Division
Fred Hutch
07.2015 - 03.2017
Bachelors of Science - Biochemistry
Seattle Pacific University
Shoreline Community College
Clark University