Virologist and Infectious Diseases Specialist
Khaled Abosria
South Dakota State University
Brookings,SD
Work Preference
Desired Job Title
MicrobiologistVirologist Research and Development Scientist
Work Type
Full Time
Location Preference
On-Site
Location:
Brookings, SD
Open to relocation:
Yes
Minimum Desired Compensation
45000/yr
Summary
Veterinary microbiologist with advanced research in molecular virology, infectious diseases, and immunology. Extensive experience in viral pathogenesis, virus-host interactions, mucosal immunity, recombinant vaccine development, and diagnostic assay design. Skilled in molecular diagnostics (PCR, in situ hybridization), serological assays (ELISA, virus neutralization), cytokine profiling, baculovirus vector systems, and phylogenetic analysis.
Work History
Graduate Student Researcher
3 Years 4 Months- Constructed and evaluated baculovirus-vectored vaccine candidates targeting multiple avian viruses to support the development of recombinant vaccine strategies.
- Verified recombinant vaccine antigen expression using Western blot and indirect fluorescent antibody testing, confirming successful expression of target viral proteins.
- Evaluated vaccine immunogenicity and efficacy in avian models through controlled vaccine administration and subsequent assessment of immune responses and protection.
- Performed molecular diagnostic and serological assays to detect and characterize viral infections and assess antibody responses following vaccination.
- Developed and optimized an ISCOM Matrix antigen-delivery platform for incorporation of viral antigens, evaluating formulation characteristics and immunogenicity to support the development of enhanced vaccine candidates.
Visiting Researcher
1 Year 2 Months- Designed and conducted controlled calf vaccination studies, administering vaccine candidates and collecting nasal and blood specimens to assess vaccine-induced mucosal and systemic immunity.
- Executed high-throughput serological and immunological analyses of bovine samples, quantifying antigen-specific mucosal and systemic immunoglobulin responses to characterize vaccine-induced humoral immunity.
- Performed virus neutralization assays against bovine respiratory viruses to measure functional antibody activity and evaluate vaccine-associated protective immunity.
- Quantified Type I and Type II interferon responses in bovine nasal secretions, characterizing local innate immune responses induced by vaccination.
- Profiled cytokine responses to identify potential immune correlates of protection, linking cellular immune responses with vaccine efficacy and protective outcomes.
Graduate Student Researcher
9 Years 9 Months- Collected and processed clinical specimens from infected animals to support timely laboratory diagnosis and molecular characterization of infectious diseases.
- Performed PCR and RT-PCR molecular diagnostics for Foot-and-Mouth Disease Virus, Sheep Pox Virus, and Lumpy Skin Disease Virus, enabling detection and identification of viral infections.
- Isolated and propagated LSDV from clinical specimens using embryonated chicken eggs, generating viral material for downstream diagnostic and research applications.
- Performed phylogenetic analysis of viral sequence data to characterize detected viruses, investigate genetic relationships, and support molecular epidemiological assessment.
- Helped with grading assignments and tests, providing constructive feedback to students based on results.
- Completed daily reports on attendance and disciplinary performance.
- Monitored student progress to identify weaknesses and provide recommendations for improvement.
- Supported classroom maintenance and upkeep, organizing books, and materials.
Education
Master of Biological Sciences
GPA: 4.0/4.0
Master of Veterinary Medical Sciences
Bachelor of Veterinary Medical Sciences
GPA: 3.73/4.0
Skills
PCR
RT-PCR
Quantitative PCR
In situ hybridization (ISH)
Molecular cloning
Plasmid preparation
Viral genome sequencing
Phylogenetic analysis
ELISA (indirect
sandwich)
Virus neutralization assays
Cytokine quantification (IFN-α
IFN-γ)
Mucosal IgA analysis
Immunoglobulin quantification
Baculovirus expression system
Recombinant protein production
Western blotting
ISCOMATRIX antigen delivery system
Adjuvants
Virus propagation
Cell culture
Serological testing
Assay development
Validation
Microsoft Office
Endnote
MEGA
Creative thinking
Project Management
Analytical Skills
Excellent Communication
Data Analysis
SOP development
Problem solving
Process Improvement
Awards
- Student travel award, North central avian diseases conference, 2024-04-01
- Student travel award, American association of avian pathologist, 2024-07-01
Research
- Infectious Diseases specialist with main focus on viral diseases in livestock. My long-term goal is to develop a unique vaccine platform that could be used in emergency vaccination in case of outbreaks
Selected Peer Reviewed Articles
- Abosria K., et al., 2025-01-01, In situ hybridization to study pathogenicity of North American avian metapneumovirus subtypes A and B in turkeys., Avian Diseases, 10.1637/aviandiseases-D-25-00070
- Abosria K., et al., 2025-01-01, Impact of turkey reoviruses on commercial flock performance., Avian Diseases, 10.1637/aviandiseases-D-24-00068
- Hosein H.I., Abosria K., et al., 2025-01-01, Genetic diversity and antigenic variability of foot-and-mouth disease virus strains in Egypt., Tropical Animal Health and Production, 10.1007/s11250-025-04275-0
- Rouby S.R., Hussein K.H., et al., 2021-01-01, Lumpy skin disease outbreaks in Egypt: Epidemiological and molecular findings., PLoS ONE, 10.1371/journal.pone.0258755
- Sherin R Rouby, Olfat Shehata, AS Abdel-Moneim, KH Hussein, MM Mahmoud, Khaled Abosria et al., 2021-01-01, Lumpy skin disease in calves: The association between clinical signs and biochemical alterations., Adv. Anim. Vet. Sci., 9, 11, 1863-1868
- Mahmoud Mohamadin, Rashid Manzoor, Samah Mosad, Sherin Rouby, Khaled Abosria et al., 2026-01-01, Evolutionary dynamics and mutational patterns of circulating foot and mouth disease virus in Egypt: phylogenetic analysis of VP1., Under review in BMC-veterinary research
Degrees
- B.V.Sc.
- M.V.Sc.
- M.S.
Training
- Infectious Disease Transmission Models for Decision-Makers, Johns Hopkins University, Baltimore, Maryland, 2025-05-01
- Working with Cattle in Agricultural Research Settings, CITI, Brookings, South Dakota, 2023-05-01
- Working with the IACUC, CITI, Brookings, South Dakota, 2023-05-01
Timeline
Graduate Student Researcher
South Dakota State University
01.2023 - 05.2026Read More
Visiting Researcher
SD-Animal diseases and Research Diagnostic Lab
10.2021 - 12.2022Read More
Graduate Student Researcher
Beni Suef University
01.2012 - 10.2021Read More
SDSU
Master of Biological Sciences
Read More
Beni-Suef University
Master of Veterinary Medical Sciences
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Beni-Suef University
Bachelor of Veterinary Medical Sciences
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