Summary
Overview
Work History
Education
Skills
Publications And Patents
Timeline
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Krishna Patel

Boston,US

Summary

Experienced scientist with 12+ years in the biotech and pharmaceutical industry. Proficient in protein purification techniques and cell biological engineering, utilizing genome modification methods. Skilled in collaborative team environments and independent work, demonstrating excellent communication abilities. Strong analytical, data interpretation, and troubleshooting skills. Well-versed in molecular biology and biochemistry techniques, plate assays, high-throughput assay development, and scientific report writing.

Overview

16
16
years of professional experience

Work History

Sr. Scientist

Moderna Therapeutics
01.2021 - Current
  • Simultaneously managed several complex projects, meeting all pertinent milestones.
  • Championed interdisciplinary collaboration across departments, fostering innovation and creative problem-solving among colleagues.
  • Early stage development of drug manufacturing specifically working on process development by implementing alternative methods of nucleic acid synthesis
  • Lead technical project development from initial proof concept stage to implementation into operations/ production facility
  • Developed innovative solutions to complex scientific problems, enhancing the company''s intellectual property portfolio.
  • Evaluated novel technologies for potential integration into existing workflows, streamlining operations while maintaining scientific rigor.
  • Led cross-functional teams for successful completion of projects on time
  • Collaborated with external partners for outsourcing work to improve efficiency and deliver project outcome in timely manner
  • Lead team of multiple scientists and research associates, provided direction and guidance on technical development of the project, supported trouble shooting and track milestone with planned timelines.
  • Participated in strategic planning efforts, helping to set both short-term and long-term goals for the organization.
  • Worked on 3 year DARPA project from conception to completion on novel nucleic acid synthesis platform and delivered yearly milestones

Organism Engineer

Ginkgo Bioworks Inc
05.2016 - 05.2021
  • Designed genetic and metabolic bio-synthetic pathways into target hosts cell applying biological and chemical principles for production of natural products
  • Employ genetic engineering tools such as CRISPR-Cas9 system, selective and marker less chromosomal integration to generate desired genomic modifications
  • Developed high throughput cell engineering platform by successful translation of small-scale model to 96 well plate/384 well plate scale
  • Protein purification using automated tip based purification techniques and semi-automated resin filled plate based techniques
  • Protein characterization assay for activity and substrate specificity using purified protein using ELISA and Octet
  • Review, analyze and evaluate data from in vitro and in vivo assays and shared using Tableau, Graphpad Prism software
  • Design strategies to overcome potential obstacles in meeting desired goals for the assigned projects
  • Provided support for development of Ginkgo's organism engineering foundry
  • Data analysis and writing scientific reports
  • Application of Molecular biology techniques including DNA assembly (TAR cloning, Gibson assembly Golden gate), NGS sequence analysis, PCR, qPCR, RT-PCR, HTP cellular assays, protein purification and protein characterization assays, genetic engineering of microbial strains at high throughput scale, bacterial growth, cell culturing and small-scale HTP assay development using automated liquid handlers
  • Actively involved in designing projects for Associates and training Interns and Associates with wet lab skills

Senior Research Associate

Manus Biosynthesis
01.2014 - 04.2016
  • Chromosomal modification on E.coli strains for over expressing heterologous genes as well as inserting native genes for up-regulation or down-regulation of pathway
  • Use of phage based recombination technique for creating library of knock out strains using Keio- collection to identify potential targets for improved small scale molecule production
  • Engineered metabolic pathways into E.coli strains using λ-red recombination techniques, CRISPR-Cas9, MAGE to generate knockout strains/knock down strains
  • Studied metabolic pathway leveraging BAC engineering
  • Development of in-house inducible/constitutive promoter libraries for heterologous protein expression
  • Characterization of genetic elements which includes promoters, insulator, terminators, spacers which were used for protein expression during molecular cloning
  • Use of techniques such as Golden Gate assembly, multi-piece Gibson assembly, multi-step type II system for plasmid construction to facilitate large pathway construction
  • Developed high throughput assay for screening engineered strains small scale fermentation assay and analytical high throughput assay
  • Analyze and tabulate data, provide results, conclusions and interpretations to project teams and customers

Research Associate

Manus Biosynthesis
07.2012 - 12.2013
  • Monitored progress against project timelines, ensuring timely completion of deliverables while maintaining high-quality standards.
  • Set up equipment, organized inventory and maintained facilities.
  • Provided technical guidance on experimental design and data interpretation issues for team members as needed.
  • Gathered, arranged, and corrected research data to create representative graphs and charts highlighting results for presentations.

Graduate Assistant

Department of Pharmaceutical Sciences, St John's University
08.2009 - 12.2012
  • Thesis research project: Investigation of the interaction between eukaryotic translation initiation proteins
  • Construction of mutant gene by site-directed mutagenesis
  • Molecular cloning to introduce mutants into inducible expression vector system
  • Optimization for ideal conditions of protein expression in E.coli
  • Expression and induction of recombinant His-tag and GST-tag mutated proteins(eukaryotic translation protein) into E.coli cells
  • Protein purification using affinity column chromatography Ni NTA and Glutathione resin and SDS-PAGE analysis for testing protein expression, solubility and purity
  • Enzyme kinetics study via ATPase assay using labeled ATP
  • Protein binding interaction study using Pull-down assay and Immuno Assays

Teaching Adjunct

Department of Microbiology, St John's University
01.2011 - 05.2012
  • Worked as adjunct professor for microbiology lab
  • Delivered lectures on microbiology and biotechnology laboratory techniques
  • Demonstrated experiments on cultivation of aerobic and anaerobic micro-organisms, identification of bacteria based on morphological staining and biochemical properties effect of antibiotic on growth of bacteria, ELISA and PCR

Intern

Vital Formularies
05.2009 - 07.2009
  • Work with the Formulation development and Manufacturing Department
  • Operate instruments like HPLC and UV Spectrophotometer in the QC department

Education

Master of Science (MS) - Pharmaceutical Sciences, Pharmacology

St. John's University
New York
01.2012

Bachelor of Science (BS) - Pharmacy

S.P. University
01.2009

Skills

  • Program management including project proposal, resource allocation, timeline draft, quarterly / annual milestone reports
  • Technology transfer cross teams
  • Cross collaboration with different teams in multi disciplinary project
  • People management
  • Novel techniques for DNA synthesis
  • High throughput assay development
  • Automated liquid handling - Hamilton, Echo, GBG, robotic arm
  • Plasmid construction - TAR cloning, Golden Gate assembly, Type IIs based assembly, Gibson assemblyType I assembly system, Seamless Gene art cloning
  • Molecular biology techniques - PCR, RT-PCR qPCR, Overlap PCR
  • Genetic engineering techniques - CRISPR-Cas9 system, Scarless chromosomal integration, Cre-lox recombination, Homologous recombination techniques, Marker selections, BAC engineering
  • Mammalian cell cultures
  • Molecular technique softwares - Vector NTI, Geneious, Clone manager
  • Data analysis softwares - Graphpad Prism, Tableau, MS Excel, JMP
  • Analytical techniques -High-throughput gel electrophoresis, fragment analyzer, Bioanalyzer, SDS PAGE, Western blot, ELISA, Octet,
  • Sequence analysis - Whole genome sequencing, Sanger data analysis, NGS data analysis
  • High throughput Protein purification techniques - Automated resin filled tip based approach, 96-well prepacked plates
  • Project management tools - Smartsheets, JIRA-Kanban board, Gantt chart

Publications And Patents

Methods for microbial production of steviol and steviol glycosides., Ajikumar Parayil Kumaran, Ryan Philippe, Jason Donald, Krishna Patel, Swati Gupta, Ryan Lim, Liwei Li, WO 2016073740 A1,


Investigation of the conserved glutamate immediately following the DEAD box in eukaryotic translation initiation factor 4AI, Patel K, Shah GK, Kommaraju SS, Low WK, Biochem Cell Biol., 92, 1, 33-42, 2014,


Harnessing Biological Complexity and Diversity through Metabolic Engineering: The Manus Bio Approach, Christine Santos, Bradley Biggs, Krishna Patel, Jason Donald, Ryan Philippe, Ajikumar Parayil, Metabolic Engineering 11, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, Manus Biosynthesis

Timeline

Sr. Scientist

Moderna Therapeutics
01.2021 - Current

Organism Engineer

Ginkgo Bioworks Inc
05.2016 - 05.2021

Senior Research Associate

Manus Biosynthesis
01.2014 - 04.2016

Research Associate

Manus Biosynthesis
07.2012 - 12.2013

Teaching Adjunct

Department of Microbiology, St John's University
01.2011 - 05.2012

Graduate Assistant

Department of Pharmaceutical Sciences, St John's University
08.2009 - 12.2012

Intern

Vital Formularies
05.2009 - 07.2009

Master of Science (MS) - Pharmaceutical Sciences, Pharmacology

St. John's University

Bachelor of Science (BS) - Pharmacy

S.P. University

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