Kumar Sachin Singh, PhD
St Louis,MO
Summary
Experienced scientist with a strong background in research and analysis. Proficient in experimental design, data analysis, and interpretation across various scientific disciplines. Demonstrated ability to contribute to impactful research projects and collaborate effectively with interdisciplinary teams. Meticulous Biochemist offering several years of experience managing laboratory experiments, equipment, and personnel. Well-versed in developing experiment protocols and reporting on findings. Proven history of applying innovative research methods to drive research progress.
Overview
8
8
years of professional experience
Work History
Staff Scientist
Washington University, St. Louis
Saint Louis, MO
07.2022 - Current
- Supervised junior scientists and laboratory technicians, providing mentorship and training.
- Designed and conducted complex experiments following established scientific protocols.
- Implemented quality control procedures to ensure the integrity of experimental data.
- Designed experiments to test hypotheses related to cell biology, biochemistry, or genetics.
- Maintained accurate records of all research activities in accordance with regulatory standards.
- Collaborated with cross-functional teams to advance research projects.
- Created and implemented new research methods to explore mechanisms of biological processes.
- Conducted literature reviews to identify cutting-edge methods for experimentation.
- Prepared presentations summarizing current research projects and results for internal stakeholders.
Postdoctoral Research Associate
Washington University In Saint Louis
Saint Louis, MO
08.2021 - 06.2022
- Established an animal model in hamsters for the life cycle of Ancylostoma ceylanicum (A. cey).
- Worked on various method and protocol for the mutagenesis using CRISPR-Cas9 in A. cey eggs and adults.
- Worked on establishing the protocol for the target finding using Click Chemistry in T. muris worm.
- Collaborated with interdisciplinary teams to enhance research methodologies and outcomes.
- Supervised laboratory personnel and delegated tasks for increased performance.
- Worked with a team of researchers to identify new targets for drug development.
- Prepared reagents according to established protocols prior to conducting experiments.
- Conducted experiments, collected data, analyzed results, and wrote reports.
Post Doc
Wistar Institute
Philadelphia, PA
06.2017 - 07.2021
- Led significant efforts in antibiotic drug discovery.
- Conducted extensive literature reviews to establish research foundation and identify gaps in current knowledge.
- Contributed to publications in peer-reviewed journals by writing manuscripts, preparing figures, and editing text.
- Presented experimental findings in presentations with graphs, charts and other visual aids.
- Coached junior staff members assigned to assist with various aspects of project implementation including data collection, analysis and report writing.
Education
Ph.D. - Life Sceince
Jawaharlal Nehru University, New Delhi
India04-2017
Master of Science - Molecular Biology And Biotechnology
Tezpur University, Assam
India06-2009
Bachelor of Science - Biotechnology
CCS University, Merut
India07-2007
Skills
- Expertise in molecular biology techniques like plasmid isolation, genomic DNA isolation, total RNA isolation, cDNA preparation, PCR cloning, ligation, transformation, electroporation, transfection, restriction digestions, Southern blotting, and construct development for knockout and knockdown of Mtb
- I have experience in the development of knock-ins in nematodes using CRISPR-Cas9
- Working experience in nematode gonadal microinjection for gene manipulation
- 15 years of post-graduate experience in the R&D sector of ARA Healthcare, Gurugram, on the process development of recombinant therapeutic protein
- Expertise in the development of recombinant strains (knockout and knockdown) of Mtb
- Expertise in the isolation and culture of mouse primary macrophages
- Expert in handling various animal cell lines, including HEK 293T cells, HEPG2, Raw cells, VERO cells, THP-1, J-774, and many cancer cell lines
- Working experience with infectious pathogens in BSL II and BSL III
- Expertise in in vivo study of Mtb infection in Balb/C mice to study cytokine profiling, CFU, RT-PCR, western blotting, and histopathology of Mtb in mouse organs such as the spleen, kidney, lung, liver, and brain
- Working experience on the animal models of various pathogens, such as Vibrio, Salmonella, Klebsiella, Yersinia, Acinetobacter, Staphylococcus, Helicobacter, Listeria, Pseudomonas, Bacillus, E coli, Mtb, Shigella, and Enterobacter
- Expertise in maintaining the life cycle of nematodes (A) ceylanicum) in hamsters
- Worked on Swiss, Balb/c, BL6, NSG, p53(S47) mutant mice, and humanized mice
- I have extensive experience working with human PBMCs and immunological analysis
- Extraction and working experience with mouse spleenocytes, experience in isolation and extraction of gdT cells from PBMCs and tissues
- Experience in prodrug cleavage and prodrug stability in plasma and microsomes using mass spectrometry
- Expertise in TEM and SEM sample preparation and image acquisition
- Experience in the preparation of wax blocks and tissue sections
- Expertise in polyclonal antibody development in rabbits and mice
- Experience in the study of phagosome maturation by single-cell image analysis
- Experienced in structured illumination-based microscopy and confocal microscopy
- Expertise in immunological techniques, like ELISA and cytokine profiling through FACS
- Expert in techniques like PCR, qRT-PCR, gene analysis, SDS-PAGE, Western blotting, and 2D gel electrophoresis, with working experience and handling of a glove box that can maintain a 05 ppm oxygen level
- Expertise in anti-mycobacterial compound screening via MABA assay, and through CFU
- Experience in recombinant therapeutic protein production, downstream processing, and purification in prokaryotes and eukaryotes
- Expertise in protein bioactivity assay development and screening of active inhibitors against the protein target
- Experience in working on iron-sulfur cluster proteins (oxygen-sensitive)
- Experience in feed-batch fermentation for recombinant therapeutics
- Have great experience in target finding using click chemistry in mammalian cell lines and nematodes
- Computer proficiency: word processor (Word), spreadsheet (Excel), and PowerPoint in Microsoft Windows; statistical and graph packages (Sigma Plot and Graph Pad), image processor (Adobe Photoshop), ImageJ, basic bioinformatics tools, and basic online database search tools
Industrial Research Experience
Worked as a Research Associateat ARA Healthcare Pvt. Ltd.Gurugram from 16 November 2009 to March 2011. Have done downstream processing of Anti-cancer Biosimilar Recombinant Therapeutic Proteins and Monoclonal Antibody and product development.
Languages
English
Professional
Hindi
Professional
Accomplishments
UGC- Senior Research Fellowship
Council of Scientific and Industrial Research & University Grants Commission, Govt. of India : April 2013-April 2015
UGC- Junior Research Fellowship
Council of Scientific and Industrial Research & University Grants Commission, Govt. of India : April 2011-April 2013.
DBT- Fellowship, Department of Biotechnology, Govt. of India : 2007-2009
Biotech Consortium of India Limited (BCIL) for industrial training, DBT, Government of India : Nov 2009- may 2010
Selected Media Coverage of the work associated with me
- https://www.sciencedaily.com/releases/2020/01/200124073908.htm
- https://www.eurekalert.org/news-releases/680976
- https://wistar.org/news/press-releases/tp53-genetic-variant-found-individuals-african-descent-linked-iron-overload-may
My work associated product in the market
- https://www.emdmillipore.com/US/en/product/Anti-IspH,MM_NF-ABF998-25UL?ReferrerURL=https%3A%2F%2Fwww.google.com%2F
- https://www.sigmaaldrich.com/US/en/product/mm/abf998
Publications
1. Samuel C Uzoechi, Bruce A Rosa, Kumar Sachin Singh, Young-Jun Choi, Bethany K Bracken, Paul J Brindley, R Reid Townsend, Robert Sprung, Bin Zhan, Maria-Elena Bottazzi, John M Hawdon, Yide Wong, Alex Loukas, Sergej Djuranovic, Makedonka Mitreva. Excretory/Secretory Proteome of Females and Males of the Hookworm Ancylostoma ceylanicum. Pathogens 12 (1), 95.
2. Singh KS, Kumar R., Chauhan A., Singh N., Sharma R, Singh D. and Singh SK (2021). DAO knockout leads to growth attenuation of Mycobacterium tuberculosis H37Ra in Balb/C mice. Tuberculosis.
3. Singh, K.S., Leu, J.IJ., Barnoud, T. et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin. Nat Commun 11, 473 (2020). https://doi.org/10.1038/s41467-019-14151-9
4. Singh K.S. and Singh S.K. (2015). The Mycobacterium tuberculosis H37Ra gene MRA_1916 causes growth defects upon downregulation. Scientific Reports 5:16131.
5. Singh K.S, Sharma R, Keshari D, Singh N and Singh SK (2017). Malate synthase of Mycobacterium tuberculosis H37Ra manages its persistence and enhances its survival by preventing phagosome-lysosome fusion. Tuberculosis106: 73-81.
6. Keshari D., Singh K.S., Sharma R., Yadav S. and Singh S.K. (2017). MSMEG_5684 down-regulation in Mycobacterium smegmatis affects its permeability, survival under stress and persistence. Tuberculosis 103: 61-70.
7. Sharma R., Keshari D., Singh K.S., Singh S. K. (2017) Biochemical and functional characterization of MRA_1571 of Mycobacterium tuberculosis H37Ra and effect of its down-regulation on survival in macrophages. Biochemical and Biophysical Research Communications. 487(4):892-897.
8. Sharma R., Keshari D., Singh K.S., Yadav S. and Singh S.K. (2016). MRA_1571 is required for isoleucine biosynthesis and improves Mycobacterium tuberculosis H37Ra survival under stress. Scientific Reports 6:27997.
9. Singh A.K., Saxena G., Dixit S., Hamidullah, Singh S.K., Singh S.K., Arshad M. and Konwar R. (2016). Synthesis, characterization and biological activities of some Ru(II) complexes with substituted chalcones and their applications as chemotherapeutics against breast cancer. Journal of molecular structur .S0022-2860(16)30070-9.
10. Modified hookworm secretes anti-tetrodotoxin human single chain antibody. Under communication in Nat. Comm.
Poster Presentation
- Participated in 2023 Molecular Helminthology meeting at the Morgridge Institute for Research Madison, Wisconsin, USA June 26-29, 2023.
- Singh K.S. and Singh S.K. (2014). Cloning, Expression and Purification studies with MRA_1916. A putative D-amino acid oxidase of Mycobacterium tuberculosis H37Ra. XXXVIII All India Cell Biology Conference and International Symposium on Cellular Response to Drugs (Dec. 10-12).
- Singh K.S. and Singh S.K. (2015). Cloning, Expression and Purification studies with MRA_1104. A putative serine hydroxymethyltransferase of Mycobacterium tuberculosisH37Ra. One day symposium on “Drug Discovery in India: Past, Present and Future”. (1jan).
- Singh K.S., Sharma R., Keshari D., Yadav S. and Singh S.K. (2015).D-amino acid oxidase knockout leads to increased susceptibility of Mycobacterium tuberculosis H37Ra under stress. New Horizoned in Biotechnology (Nov. 22-25), Thiruvananthapuram, India.
- Sharma R., Keshari D., Singh K.S., Yadav S. and Singh S.K. (2015). Down-regulation of threonindehydratase leads to increased susceptibility of Mycobacterium tuberculosis H37Ra under stress. New Horizoned in Biotechnology (Nov. 22-25), Thiruvananthapuram, India.
- Yadav S., Singh K.S., Sharma R., Keshari D. and Singh S.K. (2015). Knockdown of 42-phosphopantetheinyl transferase affects growth and increases sensitivity of Mycobacterium tuberculosis H37Ra to antimycobacterial agents. New Horizoned in Biotechnology (Nov. 22-25), Thiruvananthapuram, India.
- Keshari D., Singh K.S., Sharma R.,Yadav S. and Singh S.K. (2015).Phosphoserine aminotransferase of Mycobacterium smegmatisMC2155 maintains cell wall integrity and increases survival under stress. New Horizon in Biotechnology (Nov. 22-25), Thiruvananthapuram, India.
Workshop and hands on training
- Performed workshop on Fundamental of Bioinformatics from Tezpur University (2009), sponsored by Department of Biotechnology, Govt. of India.
- Performed a workshop on Animal handing for experimental aspect in CSIR-CDRI, Lucknow (2011).
- Training on grant writing.
- Completed training for working expertise in BSL III.
References
- Makedonka Mitreva, Ph.D., FASTMH
The Robert E. and Louise F. Dunn Professor of Medical Sciences
Professor, Departments of Medicine and of Genetics
Director, Center for Clinical Genomics of Microbial Systems
McDonnell Genome Institute, Washington University School of Medicine
[Address] 4444 Forest Park Ave., Rm 5135, Box 8501, Saint Louis, MO 63108
[P] 314-286-2005 [E] mmitreva@wustl.edu [Lab] https://mitrevalab.wustl.edu
- Dr. Sudheer Kumar Singh (PhD Supervisor)
Senior Scientist, Microbiology Division,
CSIR-Central Drug Research Institute Lucknow, INDIA.
E-mail: sudheer.vns@gmail.com
Mobile: +91-9451346150
- Dr. Rituraj Konwar, Ph D
Principal Scientist
CSIR-NEIST Jorhat, Assam INDIA
Email: rituraj.konwar@neist.res.in
Ph: +91 0376-2370121 extn 2344
Timeline
Staff Scientist
Washington University, St. Louis
07.2022 - Current
Postdoctoral Research Associate
Washington University In Saint Louis
08.2021 - 06.2022
Post Doc
Wistar Institute
06.2017 - 07.2021
Ph.D. - Life Sceince
Jawaharlal Nehru University, New Delhi
Master of Science - Molecular Biology And Biotechnology
Tezpur University, Assam
Bachelor of Science - Biotechnology
CCS University, Merut
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