Knowledgeable Research Associate with robust background in conducting and analyzing scientific studies. Contributed to significant research projects and collaborated with cross-functional teams to drive innovative solutions. Demonstrated critical thinking and proficiency in data analysis and research methodologies.
Overview
16
16
years of professional experience
Work History
Research Associate III (Clinical Service Lab)
HUDSONALPHA INSTITUTE FOR BIOTECHNOLOGY
01.2016 - Current
Achieve whole blood and saliva sample accessioning
Extract DNA from samples via manual and automated (Qiagen Qiasymphony) methods in a clinical services laboratory setting
Utilize isolated samples to perform a variety of preparations that will be used for patient diagnoses including: Taqman CNV (Copy Number Variations) and DME (Drug Metabolism) Genotyping Assays, Illumina TruSeq WGS (Whole Genome Sequencing), and Illumina GDA (Global Diversity Array) with Enhanced PGx SOP that supports adult-onset actionable disorders and pharmacogenomics research in diverse populations
Accomplish isolated genomic DNA sample accessioning, dilution, and PCR setup to accomplish Sanger sequencing and utilize the results to complete variant analysis through Mutation Surveyor software
Research Associate III (Genome Sequencing Center)
HUDSONALPHA INSTITUTE FOR BIOTECHNOLOGY
01.2009 - Current
Responsible for inoculating Bacterial Artificial Chromosome (BAC), fosmid, and transposon plates for various projects
Accomplish the extraction and purification of DNA utilizing Cosmc, Real, and Sprint Prep methods on computed-automated systems
Prepare sequencing cocktail for the amplification of purified DNA using Polymerase Chain Reaction (PCR) for ABI 3730xl analysis
Operate and maintain numerous computer-automated systems
Perform BAC, fosmid, and 40KB fosmid library construction and purification utilizing the Sanger method
Generate and purify transposon libraries according to the Qiagen QIAprep method
Employ Consed and Orchid software to accomplish BAC, fosmid, and transposon library finishing
Create clonepools with Nugen and Zymo Research protocols for Illumina sequencing
Perform 20kb template preparation using the Sage Science Blue Pippin Size Selection System to create clonepools for sequencing analysis using the Pacific Biosciences (PacBio) RSII
Construct plant DNA libraries using the Illumina TruSeq PCR-Free Single Sample and 96-Well Sample Prep, Mate Pair Library Prep, the iGenomX RIPTIDE High Throughput Rapid DNA Prep (96-well plate format), the Twist 96-Plex Library Prep, and the PacBio Whole Genome and Metagenome Library Prep methods
Train fellow research associates on library preparation processes
Education
Bachelor of Science -
University of Alabama In Huntsville
Huntsville, AL
Skills
Research and analysis
Laboratory techniques
Data Analysis
Sample Processing
Interpreting Results
Attention to Detail
Validation Testing
Accomplishments
Collaborated with team of six at the HudsonAlpha Institute to acquire the onsite Clinical Services Lab.
Timeline
Research Associate III (Clinical Service Lab)
HUDSONALPHA INSTITUTE FOR BIOTECHNOLOGY
01.2016 - Current
Research Associate III (Genome Sequencing Center)
HUDSONALPHA INSTITUTE FOR BIOTECHNOLOGY
01.2009 - Current
Bachelor of Science -
University of Alabama In Huntsville
Similar Profiles
Richard LarisRichard Laris
Research Data Coordinator at K-Biotechnology Research InstituteResearch Data Coordinator at K-Biotechnology Research Institute
Research Scientist at Singapore Institute for Food and Biotechnology Innovation (SIFBI), A*STARResearch Scientist at Singapore Institute for Food and Biotechnology Innovation (SIFBI), A*STAR
Technician in Biological and Physicochemical Assay at Institute of Biotechnology of UNAMTechnician in Biological and Physicochemical Assay at Institute of Biotechnology of UNAM