Leah Brush
Pittsburgh,PA
Summary
Offering strong foundation in scientific principles and dedication to precise and accurate work. Brings familiarity with laboratory procedures and safety standards, along with ability to quickly learn and apply new techniques. Ready to use and develop skills in data analysis and equipment handling.
Overview
2
2
years of professional experience
Work History
Intern
Hubbard LLC
05.2023 - 08.2023
- Performed ELISA testing in the serology department
- Poultry Dissection
- Isolate different types of unknown bacteria collected from environmental samples including Salmonella
- Gram stain completion and analysis
- Making agar
- Various agar plating techniques
- Analyzed problems and worked with teams to develop solutions.
- Sorted and organized files, spreadsheets, and reports.
- Completed research, compiled data, and assisted in timely reporting.
- Adhered to laboratory safety procedures to maintain compliance with quality control standards.
- Prepared samples for analysis by performing complex sample preparation tasks.
- Conducted quality control measures regularly to ensure compliance with industry standards.
Lab Technician
University of Pittsburgh
06.2024 - 12.2024
- Worked with/handled poison dart frogs.
- Animal husbandry including feeding, cleaning, and checking on frogs.
- Making fruit fly cultures.
- Sustaining fruit fly and springtail cultures.
- Maintained a safe and organized work environment to ensure safety of the specimens.
- Conducted routine inspections of animal housing areas to identify potential hazards or maintenance issues requiring attention.
Restaurant Hostess
The Restaurant At Burdicks
06.2023 - 08.2023
- Took reservations and to-go orders by phone, answered customer questions, and informed of accurate wait times.
- Managed seating chart and monitored activity in restaurant to keep constant and efficient dining flow.
- Supported servers, food runners, and bussers with keeping dining area ready for every guest.
- Answered customer questions about hours, seating, and menu information.
- Prepared dining room for service by cleaning , organizing tables and placing table settings.
- Checked in with servers to confirm preparedness before seating customers.
Education
No Degree - Biology
University of Pittsburgh
Pittsburgh, PA04.2026
Skills
- Lab Experience
- Experience in Microbiology department
- Chicken dissection
- ELISA testing
- Amphibian husbandry
- Teamwork and Collaboration
- Lab Equipment Operation
- Documentation and reporting
- Presentation Preparation
- Chemical handling
- Specimen handling
- Results Analysis
- Tissue collection
- Sterile technique (Autoclave experience)
- Agar plating/streaking experience
- Gram stain experience
- Chromatography
Accomplishments
- Conducted genome annotation and host range expansion research on Gordonia terrae's phage "Capybara," a novel genome. Data and my name will be posted on Genbank website in Spring of 2024 as a coauthor. (GenBank URL: https://www.ncbi.nlm.nih.gov/genbank/)
- University of Pittsburgh Deans List 2024 Spring semester.
- National Honors Society member.
Novel Genome Annotation Details
- Application of various software such as BLAST, GeneMark, Glimmer, Starterator, HHPred and Phamerator were used to uncover genome position, specific gene start sites, and predict gene function based off close relatives of phage Capybara.
- Conducted wet lab research of another phage named "EdnaMode" that was known to infect bacteria isolation host Gordonia terrae.
- Phage EdnaMode was serial diluted with phage buffer in a ten fold serial dilution process. Phage buffer, 100% concentrated phage, and all serial dilutions were plated on to alternate Gordonia hosts to observe possible host range expansion and infer further information concerning minor tail proteins.
- Titer and Efficiency of Plating were calculated.
Timeline
Lab Technician
University of Pittsburgh
06.2024 - 12.2024
Restaurant Hostess
The Restaurant At Burdicks
06.2023 - 08.2023
Intern
Hubbard LLC
05.2023 - 08.2023
No Degree - Biology
University of Pittsburgh
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