Leslie Santuo

• Apply, Modify and adhere to SOPs, cGMP, GLP, Quality, and Safety requirements
• Effectively execute a range of tasks, in line with SOPs, in one of the following areas:
• Downstream Activities (e.g., cell lysis, chromatography purification, centrifugation, Tangential Flow Filtration (TFF), and final formulation steps).
• Fill-Finish Activities (e.g., making buffers and solutions, and preparation of equipment and materials for final drug product).

• Complete and document quality control tests for products
• Proficient Use in HPLC and analyzing data
• Conduct tech transfer for lab test methods

• Assisted in conducting experiments to better understand the immune response to Mycobacterium tuberculosis in the macaque animal model
• Performed a wide variety of procedures and techniques including, but not limited to cell culture, clinical/diagnostic microbiology, DNA analysis, DNA isolation, DNA purification, DNA sequencing, flow cytometry, gene cloning, manipulation of radioactive isotopes, natural product isolation, organic synthesis, peptide synthesis, protein analysis, RNA isolation
• Processed primate and murine tissue and samples for downstream applications
• Performed flow cytometric cell sorting and/or analysis of research scientist-supplied sample
• Published Author on nlm.nih.gov

BACH1 promotes tissue necrosis and Mycobacterium tuberculosis susceptibility

• Performed testing as required to support method verification/validation and material & supplier qualification
• Inspected incoming raw materials and performed sampling for release testing
• Coordinated testing and shipments of samples with contract testing laboratories
• Performed identification, assay and limit testing of raw materials and packaging components to support manufacturing requirements

• Performed detailed data review for test results on assays such as ELISA, DNA/RNA extraction and gel electrophoresis, qPCR, RT-PCR, HPLC, Capillary Electrophoresis, Bioburden, endotoxin, TOC, and membrane filtration testing, generated by QC Department for compliance with cGMP, internal SOPs, and Specifications, to support production, stability studies, and lab operation

• Developed processes and techniques for protein isolation and purification under GMP compliance.
• Proficient in Processes such as TFF1, Final TFF, AKTA Ready, AKTA Pilot, Viral Filtration