LING MAO

Dynamic leader and Microbiology Department Manager at Wuhan Sunhy Biology, adept in staff management and problem-solving. Spearheaded initiatives that enhanced efficiency by streamlining sample processing, demonstrating exceptional motivational leadership and systems expertise. Proven track record in improving team performance and achieving strategic goals with innovative solutions.

• Collaborated with team of [Number] in the development of [Project name].
• Used Microsoft Excel to develop inventory tracking spreadsheets.
• Documented and resolved [Issue] which led to [Results].
• Supervised team of [Number] staff members.
• Resolved product issue through consumer testing.

1) Wentao Ding, Jian Cheng, Dan Guo, Ling Mao. Engineering the 5’UTR-Mediated regulation of protein abundance in yeast using nucleotide sequence activity relationships. ACS syntheticbiology. 2018, 7, 2709-2714

2) JiangKe Yang*, Ji-Wen Zhang, Ling Mao, Xun You, Guang-Jun Chen. Genetic modification and optimization of endo-inulinase for the enzymatic production of oligofructose from inulin. Journal of Molecular Catalysis B: Enzymatic 134(2016)225-232

3) YANG Jiang-ke*,MAO Ling, ZHOU Wen-jing, CHEN Jiang-shan, HU Chen. De

Novo Design and High-throughput Screening Strategy Achieved Over Expression of Yarrowia lipolytica Lipase YLL in Pichia patiris. China Biotechnology

4) Wen-Jing Zhou, Jiang-Ke Yang*,Ling Mao, Li-Hong Miao. 2014. Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris. Enzyme and Microbial Technology 8697. 10.007.

Patent

· YANG Jiang-ke., ZHOU Wen-jin., Mao Ling., A lipase gene COLIP and its encoded lipase.(CN 104152471 A).

· Mao Ling., Tao min., A method for producing feedstuffs by solid-fermentation with enzyme-linked microecological.( CN 106561996 A).

Mar. 2013- Mar. 2015, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

Projects include:

· Establishment of ribosome footprinting technology platform.

Using ribosome footprinting technology to explore new genes in Saccharomyces cerevisiae, and using gene knockout  technology to identify functions of new genes.

· Discover regulatory mechanism of 5'UTR on protein translation

initiation in Saccharomyces cerevisiae (National Youth Foundation of China).

Include a high-throughput 5'UTR expression and characterization system in Saccharomyces cerevisiae will be constructed, and a 50 bp random synthetic 5’UTR sequence library based on random primers mix will be expressed and characterized through this system. After that, the protein expression data collected from FACS will map to 5’UTR sequence data collected form high-throughput sequencing.

· The heat shock response of Escherichia coli  (National Science

Foundation of China).

Using the methods of proteomics to discover new genes in Escherichia coli and using gene knockout technology to identify functions of the new genes obtained above.

· Construct celery metabolism pathway in Saccharomyces cerevisiae.

Participated in exploring unknown genes of celery biosynthesis in

Saccharomyces cerevisiae; Primarily responsible for building the chassis strains of metabolic pathways, identifying functions of unknown genes and raising the yield of target product by using the method of point mutation to remove feedback inhibition.

· Clone and express Candida antarctic lipase B.

Comparing lipase genes expression of Candida antarctica with different promoters; Including the construction of heterologous recombinant expression vectors, lipase activity detection using plate method, expression product detection by SDS-PAGE and lipase activity detection under flask fermentation.